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Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah

Sulphonamides resistant strains are highly prevalent in uropathogenic Escherichia coli (UPEC) isolates. Sul genes encode sulphonamide resistance and are present on transferrable plasmids. Integrons (IGNs) are genetic elements containing integrase gene, attl site and gene cassettes which carry multip...

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Main Authors: Lin, Zaw, Yun, Mei Lai, Myo Thura Zaw, Ahmad Toha Samsudin
Format: Article
Language:English
English
Published: Bangladesh Journals OnLine (BanglaJOL) 2016
Subjects:
RC Internal medicine
Online Access:https://eprints.ums.edu.my/id/eprint/19376/1/Distribution%20of%20sul%20genes%20and%20their%20variants.pdf
https://eprints.ums.edu.my/id/eprint/19376/7/Distribution%20of%20sul%20genes%20and%20their%20variants%20in%20uropathogenic%20Escherichia%20coli.pdf
https://eprints.ums.edu.my/id/eprint/19376/
http://dx.doi.org/10.3329/ajmbr.v2i2.29063
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spelling my.ums.eprints.193762020-12-20T04:35:28Z https://eprints.ums.edu.my/id/eprint/19376/ Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah Lin, Zaw Yun, Mei Lai Myo Thura Zaw Ahmad Toha Samsudin RC Internal medicine Sulphonamides resistant strains are highly prevalent in uropathogenic Escherichia coli (UPEC) isolates. Sul genes encode sulphonamide resistance and are present on transferrable plasmids. Integrons (IGNs) are genetic elements containing integrase gene, attl site and gene cassettes which carry multiple antibiotic resistant genes. Class 1 integrons have been extensively studied because these were most prevalent among clinical isolates. In this study, UPEC isolates were determined for the antibiotic susceptibility patterns to four antibiotics commonly used for urinary tract infections, which include co-trimethoxazole (TMP-STX). Distribution of sul genes and integrase1 gene (intI1) was studied in TMP-STX resistant UPEC isolates by using multiplex polymerase chain reaction (mPCR). Sul genes variants were investigated by DNA sequencing of the whole open reading frame of sul1 and sul2 genes and PCR product of sul3 gene. Sul1, sul2 and sul3genes were prevalent in 37 (24.7%) of 150 UPEC isolates. IntI1 is positive in 22 sul genes positive isolates. Of six isolates positive with sul2 genes, sul2(a) and sul2(b) variants, which were described in the previous study, in the four isolates and the two isolates respectively were observed. This is the first mPCR which investigates the prevalence of three sul genes and intI1 in the UPEC clinical isolates from two hospitals of Sabah. Bangladesh Journals OnLine (BanglaJOL) 2016 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19376/1/Distribution%20of%20sul%20genes%20and%20their%20variants.pdf text en https://eprints.ums.edu.my/id/eprint/19376/7/Distribution%20of%20sul%20genes%20and%20their%20variants%20in%20uropathogenic%20Escherichia%20coli.pdf Lin, Zaw and Yun, Mei Lai and Myo Thura Zaw and Ahmad Toha Samsudin (2016) Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah. Asian Journal of Medical and Biological Research, 2 (2). pp. 213-220. ISSN 2412-5571 http://dx.doi.org/10.3329/ajmbr.v2i2.29063
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic RC Internal medicine
spellingShingle RC Internal medicine
Lin, Zaw
Yun, Mei Lai
Myo Thura Zaw
Ahmad Toha Samsudin
Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah
description Sulphonamides resistant strains are highly prevalent in uropathogenic Escherichia coli (UPEC) isolates. Sul genes encode sulphonamide resistance and are present on transferrable plasmids. Integrons (IGNs) are genetic elements containing integrase gene, attl site and gene cassettes which carry multiple antibiotic resistant genes. Class 1 integrons have been extensively studied because these were most prevalent among clinical isolates. In this study, UPEC isolates were determined for the antibiotic susceptibility patterns to four antibiotics commonly used for urinary tract infections, which include co-trimethoxazole (TMP-STX). Distribution of sul genes and integrase1 gene (intI1) was studied in TMP-STX resistant UPEC isolates by using multiplex polymerase chain reaction (mPCR). Sul genes variants were investigated by DNA sequencing of the whole open reading frame of sul1 and sul2 genes and PCR product of sul3 gene. Sul1, sul2 and sul3genes were prevalent in 37 (24.7%) of 150 UPEC isolates. IntI1 is positive in 22 sul genes positive isolates. Of six isolates positive with sul2 genes, sul2(a) and sul2(b) variants, which were described in the previous study, in the four isolates and the two isolates respectively were observed. This is the first mPCR which investigates the prevalence of three sul genes and intI1 in the UPEC clinical isolates from two hospitals of Sabah.
format Article
author Lin, Zaw
Yun, Mei Lai
Myo Thura Zaw
Ahmad Toha Samsudin
author_facet Lin, Zaw
Yun, Mei Lai
Myo Thura Zaw
Ahmad Toha Samsudin
author_sort Lin, Zaw
title Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah
title_short Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah
title_full Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah
title_fullStr Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah
title_full_unstemmed Distribution of sul genes and their variants in uropathogenic Escherichia coli isolated from two hospitals of Sabah
title_sort distribution of sul genes and their variants in uropathogenic escherichia coli isolated from two hospitals of sabah
publisher Bangladesh Journals OnLine (BanglaJOL)
publishDate 2016
url https://eprints.ums.edu.my/id/eprint/19376/1/Distribution%20of%20sul%20genes%20and%20their%20variants.pdf
https://eprints.ums.edu.my/id/eprint/19376/7/Distribution%20of%20sul%20genes%20and%20their%20variants%20in%20uropathogenic%20Escherichia%20coli.pdf
https://eprints.ums.edu.my/id/eprint/19376/
http://dx.doi.org/10.3329/ajmbr.v2i2.29063
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score 13.211869

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