Staff View: Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion

Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion

Aim: The cholinesterase (ChE) based inhibition studies from fish were investigated and presented here emerged to be one of the great potential biomarkers for heavy metals monitoring. Methodology: In this study, the capability of ChE extracted from the kidney of Lates calcarifer was assessed for...

Full description

Saved in:

Bibliographic Details
Main Authors:	N. M. Hayat, S. A. Ahmad, N. A. Shamaan, Mohd Khalizan Sabullah, M. Y. A. Shukor, M. A. Syed, A. Khalid, K. A. Khalil, F. A. Dahalan
Format:	Article
Language:	English
Published:	Triveni Enterprises 2017
Subjects:	QL Zoology
Online Access:	https://eprints.ums.edu.my/id/eprint/18607/1/Characterisation%20of%20cholinesterase.pdf https://eprints.ums.edu.my/id/eprint/18607/ http://www.jeb.co.in/index.php?page=abstract&issue=201705_may17&number=4
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.ums.eprints.18607
record_format	eprints
spelling	my.ums.eprints.186072018-02-03T13:52:49Z https://eprints.ums.edu.my/id/eprint/18607/ Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion N. M. Hayat S. A. Ahmad N. A. Shamaan Mohd Khalizan Sabullah M. Y. A. Shukor M. A. Syed A. Khalid K. A. Khalil F. A. Dahalan QL Zoology Aim: The cholinesterase (ChE) based inhibition studies from fish were investigated and presented here emerged to be one of the great potential biomarkers for heavy metals monitoring. Methodology: In this study, the capability of ChE extracted from the kidney of Lates calcarifer was assessed for of metal. ChE was purified through ammonium sulphate precipitation and ion exchange chromatography. Results: The purified enzyme gave 12 fold purification with the recovery of 12.17% with specific activity of 2.889 U mg-1. The Michaelis-Menten constant (Km) and Vmax value obtained was 0.1426 mM and 0.0217 �mol min-1mg-1, respectively. The enzyme has the ability to hydrolyse acetylthiocholine iodide (ATC) at a faster rate compared to other two synthetic substrates, propionylthiocholine iodide (PTC) and butyrylthiocholine iodide (BTC). ChE gave highest activity at 20-30�C in Tris-HCl buffer pH 8.0. The results showed that cholinesterase from L. calcarifer kidney was very sensitive to sensitive to copper and lead after being tested argentum, arsenic, cadmium, chromium, copper, cobalt, mercury, nickel, lead and zinc. Interpretation: The effect of heavy metals studied on the activity of ChE differed from each other. The result of the study can be used as a tool for further developing a biomarker for the detection of heavy metals in aquatic ecosystems. In addition, the information can also be used for designing a kit, that would give a rapid and accurate result. Triveni Enterprises 2017 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/18607/1/Characterisation%20of%20cholinesterase.pdf N. M. Hayat and S. A. Ahmad and N. A. Shamaan and Mohd Khalizan Sabullah and M. Y. A. Shukor and M. A. Syed and A. Khalid and K. A. Khalil and F. A. Dahalan (2017) Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion. Journal of Environmental Biology, 38 (3). pp. 383-388. ISSN 2394-0379 http://www.jeb.co.in/index.php?page=abstract&issue=201705_may17&number=4
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English
topic	QL Zoology
spellingShingle	QL Zoology N. M. Hayat S. A. Ahmad N. A. Shamaan Mohd Khalizan Sabullah M. Y. A. Shukor M. A. Syed A. Khalid K. A. Khalil F. A. Dahalan Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion
description	Aim: The cholinesterase (ChE) based inhibition studies from fish were investigated and presented here emerged to be one of the great potential biomarkers for heavy metals monitoring. Methodology: In this study, the capability of ChE extracted from the kidney of Lates calcarifer was assessed for of metal. ChE was purified through ammonium sulphate precipitation and ion exchange chromatography. Results: The purified enzyme gave 12 fold purification with the recovery of 12.17% with specific activity of 2.889 U mg-1. The Michaelis-Menten constant (Km) and Vmax value obtained was 0.1426 mM and 0.0217 �mol min-1mg-1, respectively. The enzyme has the ability to hydrolyse acetylthiocholine iodide (ATC) at a faster rate compared to other two synthetic substrates, propionylthiocholine iodide (PTC) and butyrylthiocholine iodide (BTC). ChE gave highest activity at 20-30�C in Tris-HCl buffer pH 8.0. The results showed that cholinesterase from L. calcarifer kidney was very sensitive to sensitive to copper and lead after being tested argentum, arsenic, cadmium, chromium, copper, cobalt, mercury, nickel, lead and zinc. Interpretation: The effect of heavy metals studied on the activity of ChE differed from each other. The result of the study can be used as a tool for further developing a biomarker for the detection of heavy metals in aquatic ecosystems. In addition, the information can also be used for designing a kit, that would give a rapid and accurate result.
format	Article
author	N. M. Hayat S. A. Ahmad N. A. Shamaan Mohd Khalizan Sabullah M. Y. A. Shukor M. A. Syed A. Khalid K. A. Khalil F. A. Dahalan
author_facet	N. M. Hayat S. A. Ahmad N. A. Shamaan Mohd Khalizan Sabullah M. Y. A. Shukor M. A. Syed A. Khalid K. A. Khalil F. A. Dahalan
author_sort	N. M. Hayat
title	Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion
title_short	Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion
title_full	Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion
title_fullStr	Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion
title_full_unstemmed	Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion
title_sort	characterisation of cholinesterase from kidney tissue of asian seabass (lates calcarifer) and its inhibition in presence of metal ion
publisher	Triveni Enterprises
publishDate	2017
url	https://eprints.ums.edu.my/id/eprint/18607/1/Characterisation%20of%20cholinesterase.pdf https://eprints.ums.edu.my/id/eprint/18607/ http://www.jeb.co.in/index.php?page=abstract&issue=201705_may17&number=4
_version_	1760229468906979328
score	13.211869

Characterisation of cholinesterase from kidney tissue of Asian seabass (Lates calcarifer) and its inhibition in presence of metal ion

Similar Items