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Antagonistic interaction between bacteria isolated from Antarctic snow

There are relatively few studies on the bacterial communities of the Antarctic snow. Hence, this study was set out to analyze the bacterial communities from snow and to determine whether there is any antagonistic interaction between them. Bacterial strains were isolated from Antarctic snow and we...

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Main Author: Yee, Lee Chee
Format: Academic Exercise
Language:English
Published: 2015
Online Access:https://eprints.ums.edu.my/id/eprint/18549/1/Antagonistic%20interaction.pdf
https://eprints.ums.edu.my/id/eprint/18549/
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spelling my.ums.eprints.185492018-01-29T14:59:23Z https://eprints.ums.edu.my/id/eprint/18549/ Antagonistic interaction between bacteria isolated from Antarctic snow Yee, Lee Chee There are relatively few studies on the bacterial communities of the Antarctic snow. Hence, this study was set out to analyze the bacterial communities from snow and to determine whether there is any antagonistic interaction between them. Bacterial strains were isolated from Antarctic snow and were differentiated. Forty six pure cultures were isolated and inhibitory interactions between the bacteria were tested. Initially, non redundant bacterial strains were selected through observation of the colony morphology, bacterial phenotypic characteristics and molecular approach. Of the 46 bacterial isolates, 43 strains were Gram-negative and 3 strains were Gram-positive. Three different shapes were obtained under the microscope. They were single bacilli, diplobacilli and cocci shapes. Molecular characterization was carried out using random amplified polymorphic DNA (RAPD). The RAPD data was analyzed by GelCompar II to construct phylogenetic tree. The phylogenetic relations between the 46 isolates were defined based on cluster of UPGMA. After the RAPD analysis, 10 distinct isolates were chosen for subsequent antagonistic test. All the strains were tested for antagonistic interaction on the R2A solid agar medium. Every single strain was used as 'target strain' once and the other strains were used as the 'tester strain'. All the strains tested did not show inhibitory effects toward each other. This indicates there are no antagonistic interactions between the selected strains. However, one bacteria strain was found exhibit interesting grow feature by performing autolysis when it was cultured on all bacteria strains. Studies would be needed to know more about the bacteria strain and to confirm the hypothesis made. 2015 Academic Exercise NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/18549/1/Antagonistic%20interaction.pdf Yee, Lee Chee (2015) Antagonistic interaction between bacteria isolated from Antarctic snow. Universiti Malaysia Sabah. (Unpublished)
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
description There are relatively few studies on the bacterial communities of the Antarctic snow. Hence, this study was set out to analyze the bacterial communities from snow and to determine whether there is any antagonistic interaction between them. Bacterial strains were isolated from Antarctic snow and were differentiated. Forty six pure cultures were isolated and inhibitory interactions between the bacteria were tested. Initially, non redundant bacterial strains were selected through observation of the colony morphology, bacterial phenotypic characteristics and molecular approach. Of the 46 bacterial isolates, 43 strains were Gram-negative and 3 strains were Gram-positive. Three different shapes were obtained under the microscope. They were single bacilli, diplobacilli and cocci shapes. Molecular characterization was carried out using random amplified polymorphic DNA (RAPD). The RAPD data was analyzed by GelCompar II to construct phylogenetic tree. The phylogenetic relations between the 46 isolates were defined based on cluster of UPGMA. After the RAPD analysis, 10 distinct isolates were chosen for subsequent antagonistic test. All the strains were tested for antagonistic interaction on the R2A solid agar medium. Every single strain was used as 'target strain' once and the other strains were used as the 'tester strain'. All the strains tested did not show inhibitory effects toward each other. This indicates there are no antagonistic interactions between the selected strains. However, one bacteria strain was found exhibit interesting grow feature by performing autolysis when it was cultured on all bacteria strains. Studies would be needed to know more about the bacteria strain and to confirm the hypothesis made.
format Academic Exercise
author Yee, Lee Chee
spellingShingle Yee, Lee Chee
Antagonistic interaction between bacteria isolated from Antarctic snow
author_facet Yee, Lee Chee
author_sort Yee, Lee Chee
title Antagonistic interaction between bacteria isolated from Antarctic snow
title_short Antagonistic interaction between bacteria isolated from Antarctic snow
title_full Antagonistic interaction between bacteria isolated from Antarctic snow
title_fullStr Antagonistic interaction between bacteria isolated from Antarctic snow
title_full_unstemmed Antagonistic interaction between bacteria isolated from Antarctic snow
title_sort antagonistic interaction between bacteria isolated from antarctic snow
publishDate 2015
url https://eprints.ums.edu.my/id/eprint/18549/1/Antagonistic%20interaction.pdf
https://eprints.ums.edu.my/id/eprint/18549/
_version_ 1760229460972404736
score 13.211869

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