Treatment of gill fluke disease in hybrid grouper

"Hybrid grouper is a popular marine culture fish in Sabah, Malaysia. However, the production is limited. One of the factors was due to the gill fluke occur in fish farms. Monogenean parasite was suspected as the main cause of high mortality of hybrid grouper in the fish farm. The present study...

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Bibliographic Details
Main Author: Chia, Xin Yee
Format: Academic Exercise
Language:English
Published: 2016
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Online Access:https://eprints.ums.edu.my/id/eprint/17451/1/treatment_of_gill_fluke_disease_in_hybrid_groper.pdf
https://eprints.ums.edu.my/id/eprint/17451/
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Summary:"Hybrid grouper is a popular marine culture fish in Sabah, Malaysia. However, the production is limited. One of the factors was due to the gill fluke occur in fish farms. Monogenean parasite was suspected as the main cause of high mortality of hybrid grouper in the fish farm. The present study aimed to determine causative agent of gill fluke infection in hybrid grouper at the fish farm and the effective treatment with formalin. Firstly, the gills of the unhealthy fish was cut out and observed under the microscope. The parasite was identified from characteristics of body shape and internal organs using the digital photographic microscope. 7 out of 28 fish were infected with Pseudorhabdosynochus sp. while other does not found any parasite attach on the gill. For the effect of treatment (in vitro) experiment, gill filaments with monogenean were transferred and immersed in various concentrations of formalin in the petri dish. High concentration more than 300ppm formalin was effective to gill fluke within 30 minutes. 1000ppm formalin was the most effective because all the parasites were immobilized within 10 minutes. For the acute toxicity of formalin, a total 35 healthy hybrid grouper were used in this experiment and put into 7 containers with different concentrations of formalin. The fish were exposed into each formalin for 1 hour. Formalin up to 500 ppm was not toxic to the fish. Finally, it is hoped that the finding of this study can be used to control gill fluke disease in grouper farms.