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Rapid and Sensitive E-Coli DNA Detection by Titanium Dioxide Nanoparticles

Escherichia Coli (E. coli) issue has been discovered since 1985 according to its ability to cause large outbreaks of gastrointestinal illness especially O157:H7 type. A new and simple method for label free, rapid and inexpensive of titanium dioxide (TiO2) nanoparticles biosensor based transducer on...

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Main Authors: K. F., Chong, Sh., Nadhirah, N., Zainudin, M. N. B., Derman, Ruslinda, A. Rahim
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
Q Science (General)
Online Access:http://umpir.ump.edu.my/id/eprint/9545/1/fist-2015-Rapid%20and%20Sensitive%20E-Coli.pdf
http://umpir.ump.edu.my/id/eprint/9545/
http://dx.doi.org/10.1109/IECBES.2014.7047534
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spelling my.ump.umpir.95452015-08-05T07:27:24Z http://umpir.ump.edu.my/id/eprint/9545/ Rapid and Sensitive E-Coli DNA Detection by Titanium Dioxide Nanoparticles K. F., Chong Sh., Nadhirah N., Zainudin M. N. B., Derman Ruslinda, A. Rahim Q Science (General) Escherichia Coli (E. coli) issue has been discovered since 1985 according to its ability to cause large outbreaks of gastrointestinal illness especially O157:H7 type. A new and simple method for label free, rapid and inexpensive of titanium dioxide (TiO2) nanoparticles biosensor based transducer on E. Coli DNA has been fabricated via sol-gel spin-coating technique. A simple quantitative approach was used to detect the existence of E. coli on the fabricated device. Extremely small steady current (picoammeters) were achieved, which indicates that this device can possibly go to very high sensitivities sensor towards DNA detection. A simple electrical signal of current-to-voltage (I-V) which provides small current was used to verify all the measurement of the device. (3-Aminopropyl)triethoxysilane (APTES) was functionalized through silanization process to modify the surface of TiO2 nanoparticles through the covalent bond between hydroxyl groups of TiO2 and organofunctional alkoxysilane group from APTES. The probe DNA was successfully immobilized and performed for hybridization with complementary DNA when the current is 3.5 E-10 ≤ I ≤ 4.5 E-10 A. The microchip showed reliable capture of E. coli in deionized water with an efficiency of 33.6% ± 5% at concentration of 1.0 μM. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9545/1/fist-2015-Rapid%20and%20Sensitive%20E-Coli.pdf K. F., Chong and Sh., Nadhirah and N., Zainudin and M. N. B., Derman and Ruslinda, A. Rahim (2014) Rapid and Sensitive E-Coli DNA Detection by Titanium Dioxide Nanoparticles. In: Proceedings of the Conference on Biomedical Engineering and Sciences (IECBES), 8-10 December 2014 , Miri, Sarawak. pp. 42-45.. http://dx.doi.org/10.1109/IECBES.2014.7047534
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
K. F., Chong
Sh., Nadhirah
N., Zainudin
M. N. B., Derman
Ruslinda, A. Rahim
Rapid and Sensitive E-Coli DNA Detection by Titanium Dioxide Nanoparticles
description Escherichia Coli (E. coli) issue has been discovered since 1985 according to its ability to cause large outbreaks of gastrointestinal illness especially O157:H7 type. A new and simple method for label free, rapid and inexpensive of titanium dioxide (TiO2) nanoparticles biosensor based transducer on E. Coli DNA has been fabricated via sol-gel spin-coating technique. A simple quantitative approach was used to detect the existence of E. coli on the fabricated device. Extremely small steady current (picoammeters) were achieved, which indicates that this device can possibly go to very high sensitivities sensor towards DNA detection. A simple electrical signal of current-to-voltage (I-V) which provides small current was used to verify all the measurement of the device. (3-Aminopropyl)triethoxysilane (APTES) was functionalized through silanization process to modify the surface of TiO2 nanoparticles through the covalent bond between hydroxyl groups of TiO2 and organofunctional alkoxysilane group from APTES. The probe DNA was successfully immobilized and performed for hybridization with complementary DNA when the current is 3.5 E-10 ≤ I ≤ 4.5 E-10 A. The microchip showed reliable capture of E. coli in deionized water with an efficiency of 33.6% ± 5% at concentration of 1.0 μM.
format Conference or Workshop Item
author K. F., Chong
Sh., Nadhirah
N., Zainudin
M. N. B., Derman
Ruslinda, A. Rahim
author_facet K. F., Chong
Sh., Nadhirah
N., Zainudin
M. N. B., Derman
Ruslinda, A. Rahim
author_sort K. F., Chong
title Rapid and Sensitive E-Coli DNA Detection by Titanium Dioxide Nanoparticles
title_short Rapid and Sensitive E-Coli DNA Detection by Titanium Dioxide Nanoparticles
title_full Rapid and Sensitive E-Coli DNA Detection by Titanium Dioxide Nanoparticles
title_fullStr Rapid and Sensitive E-Coli DNA Detection by Titanium Dioxide Nanoparticles
title_full_unstemmed Rapid and Sensitive E-Coli DNA Detection by Titanium Dioxide Nanoparticles
title_sort rapid and sensitive e-coli dna detection by titanium dioxide nanoparticles
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/9545/1/fist-2015-Rapid%20and%20Sensitive%20E-Coli.pdf
http://umpir.ump.edu.my/id/eprint/9545/
http://dx.doi.org/10.1109/IECBES.2014.7047534
_version_ 1822916915716161536
score 13.244414

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