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Mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation

The concept of mixed matrix membrane (MMM) chromatography is an alternative that provides a simple method for the preparation of adsorptive membranes, yet results in high quality membrane chromatography performance. MMMs are prepared by incorporating an ion exchange resin (or any adsorptive resin) i...

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Main Author: Siti Nor Ainda, Mazlan
Format: Undergraduates Project Papers
Language:English
Published: 2014
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/9234/1/cd8654.pdf
http://umpir.ump.edu.my/id/eprint/9234/
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spelling my.ump.umpir.92342021-07-16T00:49:36Z http://umpir.ump.edu.my/id/eprint/9234/ Mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation Siti Nor Ainda, Mazlan TP Chemical technology The concept of mixed matrix membrane (MMM) chromatography is an alternative that provides a simple method for the preparation of adsorptive membranes, yet results in high quality membrane chromatography performance. MMMs are prepared by incorporating an ion exchange resin (or any adsorptive resin) into a membrane polymer solution prior to membrane casting. In this study, MMM will be prepared by combining cation and anion simultaneously in EVAL based polymer solution. The membrane will be used for whey protein fractionation to recover both acidic and basic protein. Whey is the liquid byproduct of casein precipitation of milk in the cheese or casein manufacturing industries and of milk concentration prior to milk powder production (Saufi, 2010). Rather than using the high cost ion exchange resin that purposely design for protein fractionation, this study will using a low cost ion exchanger resin that commonly used at the industry. The 7.5 wt% anion resin (MP500) will be bland with 7.5 wt% cation resin (CNP105) in the 15wt% EVAL based polymer solution. This mixed mode interaction MMM will applied for whey protein fractionation. The feasibility of MMM was tested in whey protein fractionation processes. WPI solutions were diluted to 2mg/mL with different pH binding buffer solution (pH 4, 5, 6, 7, and 8). The whey protein compositions after binding were assayed using UPLC column ACQUITY UPLC PrST using a C4 Jupiter column. A combination of pH and salt elution using different pH elution buffers will be performed to selectively elute the bound protein on the membrane. This membrane had a maximum static binding capacity of 0.260 mg/mL β-Lac, 0.214 mg/mL BSA, and 0.118 mg/mL α-Lac per membrane at pH 5. The membrane was successfully applied to bind acidic and basic proteins simultaneously from a mixture. Based on batch elution experimental results, a productivity of 0.156 mg whey protein mL-1 membrane h-1 was calculated using this customized mixed mode MMM 2014-07 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9234/1/cd8654.pdf Siti Nor Ainda, Mazlan (2014) Mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Siti Nor Ainda, Mazlan
Mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation
description The concept of mixed matrix membrane (MMM) chromatography is an alternative that provides a simple method for the preparation of adsorptive membranes, yet results in high quality membrane chromatography performance. MMMs are prepared by incorporating an ion exchange resin (or any adsorptive resin) into a membrane polymer solution prior to membrane casting. In this study, MMM will be prepared by combining cation and anion simultaneously in EVAL based polymer solution. The membrane will be used for whey protein fractionation to recover both acidic and basic protein. Whey is the liquid byproduct of casein precipitation of milk in the cheese or casein manufacturing industries and of milk concentration prior to milk powder production (Saufi, 2010). Rather than using the high cost ion exchange resin that purposely design for protein fractionation, this study will using a low cost ion exchanger resin that commonly used at the industry. The 7.5 wt% anion resin (MP500) will be bland with 7.5 wt% cation resin (CNP105) in the 15wt% EVAL based polymer solution. This mixed mode interaction MMM will applied for whey protein fractionation. The feasibility of MMM was tested in whey protein fractionation processes. WPI solutions were diluted to 2mg/mL with different pH binding buffer solution (pH 4, 5, 6, 7, and 8). The whey protein compositions after binding were assayed using UPLC column ACQUITY UPLC PrST using a C4 Jupiter column. A combination of pH and salt elution using different pH elution buffers will be performed to selectively elute the bound protein on the membrane. This membrane had a maximum static binding capacity of 0.260 mg/mL β-Lac, 0.214 mg/mL BSA, and 0.118 mg/mL α-Lac per membrane at pH 5. The membrane was successfully applied to bind acidic and basic proteins simultaneously from a mixture. Based on batch elution experimental results, a productivity of 0.156 mg whey protein mL-1 membrane h-1 was calculated using this customized mixed mode MMM
format Undergraduates Project Papers
author Siti Nor Ainda, Mazlan
author_facet Siti Nor Ainda, Mazlan
author_sort Siti Nor Ainda, Mazlan
title Mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation
title_short Mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation
title_full Mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation
title_fullStr Mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation
title_full_unstemmed Mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation
title_sort mixed matrix membrane chromatography based on lewatit mp500 anion exchanger resin and lewatit cnp105 cation exchanger resin for whey protein fractionation
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/9234/1/cd8654.pdf
http://umpir.ump.edu.my/id/eprint/9234/
_version_ 1706957195681202176
score 13.211869

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