Mixtures of S. Rebaudiana Leaves and Stems Waste for Steviosides Synthesis Using Ethanol-Water Solvents

Mixtures of S.rebaudiana leaves and stems waste were applied for steviosides synthesis. The various ethanol-water as polarity solvents were employed in the ultrasound-assisted extraction (UAE). The HPLC (High Performance Liquid Chromatography) with Eurospher 100–5NH2 column at 1mL/m3 were used for s...

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Bibliographic Details
Main Authors: Said, N., Zulkarnain, Jema'at, Nurfariha Fathmi, Zakaria, Siti Farhana, Haron, N. S., Ghazali, Fatimah A., Misebah
Format: Article
Language:English
Published: Malaysian Technical Universities Network (MTUN) 2014
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Online Access:http://umpir.ump.edu.my/id/eprint/9071/1/Mixtures%20Of%20S.%20Rebaudiana%20Leaves%20And%20Stems%20Waste%20For%20Steviosides%20Synthesis%20Using%20Ethanol-Water%20Solvents.pdf
http://umpir.ump.edu.my/id/eprint/9071/
http://journal.utem.edu.my/index.php/jet/article/view/161
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Summary:Mixtures of S.rebaudiana leaves and stems waste were applied for steviosides synthesis. The various ethanol-water as polarity solvents were employed in the ultrasound-assisted extraction (UAE). The HPLC (High Performance Liquid Chromatography) with Eurospher 100–5NH2 column at 1mL/m3 were used for steviosides analysis. The maximum yields were achieved by the time of 30 min., temperature of 40oC and ethanol-water ratio of 80:20 (v/v). The second order kinetics model best fits the obtained data with R2 and k of 0.992 and 0.02 min-1. Utilization of wastes as an alternative feed stock provides a cost-effective and environmental friendly way for steviosides extraction.More extensive investigation needs to be performed to compare the various advantageous processing and technical feasibilty.