Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1

The cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) from Bacillus sp. TS1-1 has been partially purified from crude culture by centrifugation and cross-flow filtration. Initial 72.96 U/ml of CGTase was detected in the culture after 24 hours of incubation. The crude supernatant obtained after ce...

Full description

Saved in:
Bibliographic Details
Main Author: Mohd Azim, Abdul Rahman
Format: Undergraduates Project Papers
Language:English
Published: 2008
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/792/1/Mohd_Azim_Abdul_Rahman.pdf
http://umpir.ump.edu.my/id/eprint/792/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ump.umpir.792
record_format eprints
spelling my.ump.umpir.7922021-06-04T08:50:18Z http://umpir.ump.edu.my/id/eprint/792/ Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1 Mohd Azim, Abdul Rahman TP Chemical technology The cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) from Bacillus sp. TS1-1 has been partially purified from crude culture by centrifugation and cross-flow filtration. Initial 72.96 U/ml of CGTase was detected in the culture after 24 hours of incubation. The crude supernatant obtained after centrifugation for 5000 rpm, 5 minutes and 4°C was subsequently filtered at 15°C through cross-flow filtration using Kvick Lab cross-flow system. Two cassettes were used with molecular weight cut off of 50K and 10K. The retentate from 50K cassette was further filtered through 10K cassette. Each permeate and retentate from each cassette were tested for CGTase activity. The highest CGTase activity was detected at retentate of 10K cassette which suggested an initial size of CGTase between 10 kDa and 50 kDa. The crude and partially purified enzyme was then subjected to electrophoresis (SDS-PAGE). It was clear that a large amount of proteins had been removed by cross-flow filtration. Based on this work, the crude enzyme was purified 5.57 fold using cross-flow filtration. 2008-05 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/792/1/Mohd_Azim_Abdul_Rahman.pdf Mohd Azim, Abdul Rahman (2008) Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mohd Azim, Abdul Rahman
Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1
description The cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) from Bacillus sp. TS1-1 has been partially purified from crude culture by centrifugation and cross-flow filtration. Initial 72.96 U/ml of CGTase was detected in the culture after 24 hours of incubation. The crude supernatant obtained after centrifugation for 5000 rpm, 5 minutes and 4°C was subsequently filtered at 15°C through cross-flow filtration using Kvick Lab cross-flow system. Two cassettes were used with molecular weight cut off of 50K and 10K. The retentate from 50K cassette was further filtered through 10K cassette. Each permeate and retentate from each cassette were tested for CGTase activity. The highest CGTase activity was detected at retentate of 10K cassette which suggested an initial size of CGTase between 10 kDa and 50 kDa. The crude and partially purified enzyme was then subjected to electrophoresis (SDS-PAGE). It was clear that a large amount of proteins had been removed by cross-flow filtration. Based on this work, the crude enzyme was purified 5.57 fold using cross-flow filtration.
format Undergraduates Project Papers
author Mohd Azim, Abdul Rahman
author_facet Mohd Azim, Abdul Rahman
author_sort Mohd Azim, Abdul Rahman
title Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1
title_short Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1
title_full Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1
title_fullStr Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1
title_full_unstemmed Partial purification of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1
title_sort partial purification of cyclodextrin glucanotransferase from alkalophilic bacillus sp. ts1-1
publishDate 2008
url http://umpir.ump.edu.my/id/eprint/792/1/Mohd_Azim_Abdul_Rahman.pdf
http://umpir.ump.edu.my/id/eprint/792/
_version_ 1702169997548191744
score 13.211869