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Isolation and purification of pinellic acid from Pinellia Ternata

Pinellia ternata is a perennial herb belonging to the Araceae family. Pinellia ternata is a plant native to Japan, but also grows as a weed in certain areas of North America. It is commonly used as one of the main components in many decoctions in traditional Chinese medicine that has been used wid...

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Main Author: Cornelia, Chin Siew Ling
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/7784/1/CORNELIA_CHIN_SIEW_LING.PDF
http://umpir.ump.edu.my/id/eprint/7784/
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spelling my.ump.umpir.77842021-03-18T08:04:47Z http://umpir.ump.edu.my/id/eprint/7784/ Isolation and purification of pinellic acid from Pinellia Ternata Cornelia, Chin Siew Ling TP Chemical technology Pinellia ternata is a perennial herb belonging to the Araceae family. Pinellia ternata is a plant native to Japan, but also grows as a weed in certain areas of North America. It is commonly used as one of the main components in many decoctions in traditional Chinese medicine that has been used widely for thousands of years in China to prevent vomiting and for analgesic and sedative effects. Pinellia ternata is an active herbal component of the traditional Japanese herbal (Kampo) medicine, Sho-seiryu-to (SST) (Chinese name: Xiao-Qing-Long-Tang), and it has been reported to show oral adjuvant activity for nasally administered influenza hemagglutinin (HA) vaccine. The active compound that displayed adjuvant properties was identified as 9S, 12S, 13S-trihydroxy-1OEoctadecenoic acid commonly known as Pinellic acid. This research was conducted with a goal to isolate and purify Pinellic Acid (9S, 12S, 13S-trihydroxy-1OEoctadecenoic acid) from Pinellia ternata plant. Pinellia ternata was extracted with two types of solvent; water and methanol. The extracts were purified with Hydrophobic Interaction Chromatography with methanol as the polar eluent and Column Chromatography with the solvent system hexane-ethyl acetate. The pure Pinellic acid obtained was determined by Reversed phase High Performance Liquid Chromatography (RP-HPLC) using 70% methanol containing 0.01% acetic acid as the solvent system; and Infrared Spectroscopy. Highest % yield of Pinellic acid calculated was obtained from the sample extracted with methanol. High Performance Liquid Chromatography analysis for methanol-extract gave a clear single peak at 4.11 minutes at a flow rate of 1 ml/min. The compound obtained has displayed solvent selectivity towards methanol as opposed to water. 2012 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7784/1/CORNELIA_CHIN_SIEW_LING.PDF Cornelia, Chin Siew Ling (2012) Isolation and purification of pinellic acid from Pinellia Ternata. Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Cornelia, Chin Siew Ling
Isolation and purification of pinellic acid from Pinellia Ternata
description Pinellia ternata is a perennial herb belonging to the Araceae family. Pinellia ternata is a plant native to Japan, but also grows as a weed in certain areas of North America. It is commonly used as one of the main components in many decoctions in traditional Chinese medicine that has been used widely for thousands of years in China to prevent vomiting and for analgesic and sedative effects. Pinellia ternata is an active herbal component of the traditional Japanese herbal (Kampo) medicine, Sho-seiryu-to (SST) (Chinese name: Xiao-Qing-Long-Tang), and it has been reported to show oral adjuvant activity for nasally administered influenza hemagglutinin (HA) vaccine. The active compound that displayed adjuvant properties was identified as 9S, 12S, 13S-trihydroxy-1OEoctadecenoic acid commonly known as Pinellic acid. This research was conducted with a goal to isolate and purify Pinellic Acid (9S, 12S, 13S-trihydroxy-1OEoctadecenoic acid) from Pinellia ternata plant. Pinellia ternata was extracted with two types of solvent; water and methanol. The extracts were purified with Hydrophobic Interaction Chromatography with methanol as the polar eluent and Column Chromatography with the solvent system hexane-ethyl acetate. The pure Pinellic acid obtained was determined by Reversed phase High Performance Liquid Chromatography (RP-HPLC) using 70% methanol containing 0.01% acetic acid as the solvent system; and Infrared Spectroscopy. Highest % yield of Pinellic acid calculated was obtained from the sample extracted with methanol. High Performance Liquid Chromatography analysis for methanol-extract gave a clear single peak at 4.11 minutes at a flow rate of 1 ml/min. The compound obtained has displayed solvent selectivity towards methanol as opposed to water.
format Undergraduates Project Papers
author Cornelia, Chin Siew Ling
author_facet Cornelia, Chin Siew Ling
author_sort Cornelia, Chin Siew Ling
title Isolation and purification of pinellic acid from Pinellia Ternata
title_short Isolation and purification of pinellic acid from Pinellia Ternata
title_full Isolation and purification of pinellic acid from Pinellia Ternata
title_fullStr Isolation and purification of pinellic acid from Pinellia Ternata
title_full_unstemmed Isolation and purification of pinellic acid from Pinellia Ternata
title_sort isolation and purification of pinellic acid from pinellia ternata
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/7784/1/CORNELIA_CHIN_SIEW_LING.PDF
http://umpir.ump.edu.my/id/eprint/7784/
_version_ 1695530950347194368
score 13.211869

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