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Performance of red pigment production by Monascus purpureus at different extraction methods

The purpose of this research is mainly to study the performance of red pigment production at different extraction methods. The strain used in this study is Monascus purpureus which produce red pigment and red yeast rice was used as the substrate in the solid state fermentation. The initial moisture...

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Main Author: Sharifah Shufiza Khaziri, Tuan Hadi
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/7229/1/Performance_of_red_pigment_production_by_Monascus_purpureus.pdf
http://umpir.ump.edu.my/id/eprint/7229/
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spelling my.ump.umpir.72292021-06-09T03:07:41Z http://umpir.ump.edu.my/id/eprint/7229/ Performance of red pigment production by Monascus purpureus at different extraction methods Sharifah Shufiza Khaziri, Tuan Hadi TP Chemical technology The purpose of this research is mainly to study the performance of red pigment production at different extraction methods. The strain used in this study is Monascus purpureus which produce red pigment and red yeast rice was used as the substrate in the solid state fermentation. The initial moisture content in fermentation would be 56% which was considered as optimal pigmentation to be occurred. It would then be inoculated with 1x107 spore/ml suspension of inoculum. Extraction methods including solvent extraction, Soxhlet extraction, agitated extraction and ultrasonic extraction were being carried out after fermentation process complete. Each methods required several conditions to be fulfilled to achieve the objective of this research. In this study, the best solvent used was 60% ethyl alcohol. Uv-Vis spectrophotometer is used to analyze red pigments in the extracts which were quantified by reading the absorbance at wavelength of 500 nm for this pigment. Thus, the best performance of the methods was identified by maximum extracts achieved which was measured by concentration of extracted product obtained. Solvent extraction was used as the reference and this method produced 1.278AU/g.d pigment extract. The results were 1.268AU/g.d for ultrasonic extraction, 1.265AU/g.d for agitated extraction and 0.128AU/g.d for Soxhlet extraction. The best method was ultrasonic extraction as compared to solvent extraction in a separate experiment and it produced 0.920AU/g.d than in solvent extraction, 0.869 AU/g.d pigment extract. The increrment percentage was about 5.81%. Besides, an analysis of variance (ANOVA) was carried out to investigate the significance to use the methods, showing the p-value. The p-value for agitated extraction was 3.63x10-7, 8.35x10-8 for ultrasonic extraction and 3.79x10-7 for Soxhlet extraction. All of the methods were significant to be carried out since all of the p-value was lower than 0.05. 2013 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7229/1/Performance_of_red_pigment_production_by_Monascus_purpureus.pdf Sharifah Shufiza Khaziri, Tuan Hadi (2013) Performance of red pigment production by Monascus purpureus at different extraction methods. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Sharifah Shufiza Khaziri, Tuan Hadi
Performance of red pigment production by Monascus purpureus at different extraction methods
description The purpose of this research is mainly to study the performance of red pigment production at different extraction methods. The strain used in this study is Monascus purpureus which produce red pigment and red yeast rice was used as the substrate in the solid state fermentation. The initial moisture content in fermentation would be 56% which was considered as optimal pigmentation to be occurred. It would then be inoculated with 1x107 spore/ml suspension of inoculum. Extraction methods including solvent extraction, Soxhlet extraction, agitated extraction and ultrasonic extraction were being carried out after fermentation process complete. Each methods required several conditions to be fulfilled to achieve the objective of this research. In this study, the best solvent used was 60% ethyl alcohol. Uv-Vis spectrophotometer is used to analyze red pigments in the extracts which were quantified by reading the absorbance at wavelength of 500 nm for this pigment. Thus, the best performance of the methods was identified by maximum extracts achieved which was measured by concentration of extracted product obtained. Solvent extraction was used as the reference and this method produced 1.278AU/g.d pigment extract. The results were 1.268AU/g.d for ultrasonic extraction, 1.265AU/g.d for agitated extraction and 0.128AU/g.d for Soxhlet extraction. The best method was ultrasonic extraction as compared to solvent extraction in a separate experiment and it produced 0.920AU/g.d than in solvent extraction, 0.869 AU/g.d pigment extract. The increrment percentage was about 5.81%. Besides, an analysis of variance (ANOVA) was carried out to investigate the significance to use the methods, showing the p-value. The p-value for agitated extraction was 3.63x10-7, 8.35x10-8 for ultrasonic extraction and 3.79x10-7 for Soxhlet extraction. All of the methods were significant to be carried out since all of the p-value was lower than 0.05.
format Undergraduates Project Papers
author Sharifah Shufiza Khaziri, Tuan Hadi
author_facet Sharifah Shufiza Khaziri, Tuan Hadi
author_sort Sharifah Shufiza Khaziri, Tuan Hadi
title Performance of red pigment production by Monascus purpureus at different extraction methods
title_short Performance of red pigment production by Monascus purpureus at different extraction methods
title_full Performance of red pigment production by Monascus purpureus at different extraction methods
title_fullStr Performance of red pigment production by Monascus purpureus at different extraction methods
title_full_unstemmed Performance of red pigment production by Monascus purpureus at different extraction methods
title_sort performance of red pigment production by monascus purpureus at different extraction methods
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7229/1/Performance_of_red_pigment_production_by_Monascus_purpureus.pdf
http://umpir.ump.edu.my/id/eprint/7229/
_version_ 1702170073957924864
score 13.211869

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