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Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer

Chromatography technique is widely used for protein separation. Conventional packed bed column chromatography has several limitations. Membrane chromatography was a suitable alternative technique for protein separation. Specific monomer can be grafted to uncharged membrane to transform into membrane...

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Main Author: Lee, Yue Wei
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
QD Chemistry
Online Access:http://umpir.ump.edu.my/id/eprint/7169/1/Development%20of%20anion-exchange%20membrane%20chromatography%20from%20regenerated%20cellulose%20membrane%20by%20attaching%20different%20spacer%20arm%20length%20of%20diamine%20monomer.pdf
http://umpir.ump.edu.my/id/eprint/7169/
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spelling my.ump.umpir.71692023-07-18T01:38:27Z http://umpir.ump.edu.my/id/eprint/7169/ Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer Lee, Yue Wei QD Chemistry Chromatography technique is widely used for protein separation. Conventional packed bed column chromatography has several limitations. Membrane chromatography was a suitable alternative technique for protein separation. Specific monomer can be grafted to uncharged membrane to transform into membrane chromatography material. Optimization of parameters involve during this chemical modification is crucial for the development of high performance membrane chromatography for protein separation. The purpose of this research is to develop anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm lengths of diamine monomer. Regenerated cellulose membrane was activated in a solution containing sodium hydroxide (NaOH) and epichlorohydrin (EPI). Then, the membrane was immersed in diamine solution of 1,2-diaminoethane or 1,4-diaminobutanhe to produce positively charged membrane chromatography. The concentration of NaOH activation from 0.05M to 0.50M and diamine monomer concentration from 0.25M to 2.0M during grafting were studied. The optimum concentration of NaOH was 0.20M which produced anion exchange membrane capacity of 0.310±0.033 mgBSA/cm2 membrane. High concentration of diamine monomer at 2.0M 1,4-diaminobutane showed a binding capacity of 0.385±0.027mgBSA/cm2 membrane. Based on FTIR transmission peak, both N-H and C-N functional groups were detected in modified membrane that indicated the successful of grafting process. 2013 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/7169/1/Development%20of%20anion-exchange%20membrane%20chromatography%20from%20regenerated%20cellulose%20membrane%20by%20attaching%20different%20spacer%20arm%20length%20of%20diamine%20monomer.pdf Lee, Yue Wei (2013) Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Lee, Yue Wei
Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
description Chromatography technique is widely used for protein separation. Conventional packed bed column chromatography has several limitations. Membrane chromatography was a suitable alternative technique for protein separation. Specific monomer can be grafted to uncharged membrane to transform into membrane chromatography material. Optimization of parameters involve during this chemical modification is crucial for the development of high performance membrane chromatography for protein separation. The purpose of this research is to develop anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm lengths of diamine monomer. Regenerated cellulose membrane was activated in a solution containing sodium hydroxide (NaOH) and epichlorohydrin (EPI). Then, the membrane was immersed in diamine solution of 1,2-diaminoethane or 1,4-diaminobutanhe to produce positively charged membrane chromatography. The concentration of NaOH activation from 0.05M to 0.50M and diamine monomer concentration from 0.25M to 2.0M during grafting were studied. The optimum concentration of NaOH was 0.20M which produced anion exchange membrane capacity of 0.310±0.033 mgBSA/cm2 membrane. High concentration of diamine monomer at 2.0M 1,4-diaminobutane showed a binding capacity of 0.385±0.027mgBSA/cm2 membrane. Based on FTIR transmission peak, both N-H and C-N functional groups were detected in modified membrane that indicated the successful of grafting process.
format Undergraduates Project Papers
author Lee, Yue Wei
author_facet Lee, Yue Wei
author_sort Lee, Yue Wei
title Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_short Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_full Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_fullStr Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_full_unstemmed Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_sort development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7169/1/Development%20of%20anion-exchange%20membrane%20chromatography%20from%20regenerated%20cellulose%20membrane%20by%20attaching%20different%20spacer%20arm%20length%20of%20diamine%20monomer.pdf
http://umpir.ump.edu.my/id/eprint/7169/
_version_ 1772811329206222848
score 13.211869

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