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Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer

Chromatographic separation of protein mixtures has become one of the most effective widely used means of techniques to purifying individual proteins. Packed bed chromatography is the common technique that is used configuration for the protein separation. However, packed bed chromatography has its so...

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Main Author: Wan Najibah, Mohamad
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
QD Chemistry
Online Access:http://umpir.ump.edu.my/id/eprint/7159/1/CD7145.pdf
http://umpir.ump.edu.my/id/eprint/7159/
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spelling my.ump.umpir.71592021-06-03T05:18:00Z http://umpir.ump.edu.my/id/eprint/7159/ Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer Wan Najibah, Mohamad QD Chemistry Chromatographic separation of protein mixtures has become one of the most effective widely used means of techniques to purifying individual proteins. Packed bed chromatography is the common technique that is used configuration for the protein separation. However, packed bed chromatography has its some limitations during separation process such as high pressure drop and time consuming. Membrane chromatography then introduced to overcome the limitations of the packed bed chromatography. In the current research, polyethersulfone (PES) commercial membrane was converted into ion exchange (IEX) membrane chromatography by attaching [2-(acryloyloxy)ethyl] trimethyl ammonium chloride (AETMA) and acrylic acid (AA) monomer using UV light irradiation technique. The effect of AETMA and AA monomer concentration from 1.5 M to 2.0 M was studied. The IEX membrane was characterized in term of degree of grafting, changed of functional group as well as protein binding capacity using pure bovine serum albumin. For AETMA-grafted membrane, the binding capacity increase about the 65.32 % as the monomer concentration increase from 1.5 M to 2.0 M. While, for AA-grafted membrane, the binding capacity shows a huge increment when the monomer concentration was increase from 1.5 M to 2.0 M with amount 78.65 %. 2013-02 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7159/1/CD7145.pdf Wan Najibah, Mohamad (2013) Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Wan Najibah, Mohamad
Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer
description Chromatographic separation of protein mixtures has become one of the most effective widely used means of techniques to purifying individual proteins. Packed bed chromatography is the common technique that is used configuration for the protein separation. However, packed bed chromatography has its some limitations during separation process such as high pressure drop and time consuming. Membrane chromatography then introduced to overcome the limitations of the packed bed chromatography. In the current research, polyethersulfone (PES) commercial membrane was converted into ion exchange (IEX) membrane chromatography by attaching [2-(acryloyloxy)ethyl] trimethyl ammonium chloride (AETMA) and acrylic acid (AA) monomer using UV light irradiation technique. The effect of AETMA and AA monomer concentration from 1.5 M to 2.0 M was studied. The IEX membrane was characterized in term of degree of grafting, changed of functional group as well as protein binding capacity using pure bovine serum albumin. For AETMA-grafted membrane, the binding capacity increase about the 65.32 % as the monomer concentration increase from 1.5 M to 2.0 M. While, for AA-grafted membrane, the binding capacity shows a huge increment when the monomer concentration was increase from 1.5 M to 2.0 M with amount 78.65 %.
format Undergraduates Project Papers
author Wan Najibah, Mohamad
author_facet Wan Najibah, Mohamad
author_sort Wan Najibah, Mohamad
title Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer
title_short Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer
title_full Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer
title_fullStr Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer
title_full_unstemmed Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer
title_sort preparation of ion exchange membrane chromatography by modification of polyethersulfone membrane through uv grafting of [2-(acryloyloxy) ethyl] trimethyl ammonium chloride and acrylic acid monomer
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7159/1/CD7145.pdf
http://umpir.ump.edu.my/id/eprint/7159/
_version_ 1702170064405397504
score 13.211869

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