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Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol

The mixture of s.rebaudiana stems and leaves will be extracted to get the stevioside. Stevioside is a diterpene steviol Glycoside which is produced by conventional process. It is used to sweeten soft drink,soya sauce, yoghurt and other food. It also good for diabetic patients. This research objectiv...

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Main Author: Nurfariha Fathmi, Zakaria
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/7086/1/16.Application%20of%20ultrasound%20technique%20for%20Steviol%20Glycosides%20separation%20from%20mixtures%20of%20S.Rebaudiana%20stems%20and%20leaves%20using%20methanol.pdf
http://umpir.ump.edu.my/id/eprint/7086/
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spelling my.ump.umpir.70862023-04-14T00:26:12Z http://umpir.ump.edu.my/id/eprint/7086/ Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol Nurfariha Fathmi, Zakaria TP Chemical technology The mixture of s.rebaudiana stems and leaves will be extracted to get the stevioside. Stevioside is a diterpene steviol Glycoside which is produced by conventional process. It is used to sweeten soft drink,soya sauce, yoghurt and other food. It also good for diabetic patients. This research objective is to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves using ultrasound extraction technique and find their applications. The process normally include the extraction, pre-treatment, separation, purification and refining. These processes will use ultrasound technique and followed by High performance liquid chromatography analysis (HPLC). Ultrasound extraction technique is one of the techniques to extract the mixture of s.rebaudiana stems and leaves before the sample of the the mixture of s.rebaudiana stems and leaves is analyzed by HPLC. The mixture of s.rebaudiana stems and leaves will be in dry and powder condition before it extracted by ultrasound extraction technique using methanol as a solvent. Power level that used in the extraction is 280 W and the time range is between 10min to 50min at temperature range of 200C to 600C. After that the analysis was performed using High performance liquid chromatography (HPLC). Using 30 min, 40 0C and 80% methanol is the most suitable time, temperature and percent of solvent used to break the analyte and matrix bond and produce the steviol Glycoside in optimum yields. From this research we know that ultrasound extraction technique is most common technique and most suitable in order to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves. The optimum yield can afforded at extraction time of 30 minutes, extraction temperature of 40 0C and the percentage of methanol of 80%. Extraction kinetic of steviol glycosides approved the second-order kinetic yielding good R2 values of 0.997 and k values of 0.0301. 2013 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/7086/1/16.Application%20of%20ultrasound%20technique%20for%20Steviol%20Glycosides%20separation%20from%20mixtures%20of%20S.Rebaudiana%20stems%20and%20leaves%20using%20methanol.pdf Nurfariha Fathmi, Zakaria (2013) Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nurfariha Fathmi, Zakaria
Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol
description The mixture of s.rebaudiana stems and leaves will be extracted to get the stevioside. Stevioside is a diterpene steviol Glycoside which is produced by conventional process. It is used to sweeten soft drink,soya sauce, yoghurt and other food. It also good for diabetic patients. This research objective is to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves using ultrasound extraction technique and find their applications. The process normally include the extraction, pre-treatment, separation, purification and refining. These processes will use ultrasound technique and followed by High performance liquid chromatography analysis (HPLC). Ultrasound extraction technique is one of the techniques to extract the mixture of s.rebaudiana stems and leaves before the sample of the the mixture of s.rebaudiana stems and leaves is analyzed by HPLC. The mixture of s.rebaudiana stems and leaves will be in dry and powder condition before it extracted by ultrasound extraction technique using methanol as a solvent. Power level that used in the extraction is 280 W and the time range is between 10min to 50min at temperature range of 200C to 600C. After that the analysis was performed using High performance liquid chromatography (HPLC). Using 30 min, 40 0C and 80% methanol is the most suitable time, temperature and percent of solvent used to break the analyte and matrix bond and produce the steviol Glycoside in optimum yields. From this research we know that ultrasound extraction technique is most common technique and most suitable in order to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves. The optimum yield can afforded at extraction time of 30 minutes, extraction temperature of 40 0C and the percentage of methanol of 80%. Extraction kinetic of steviol glycosides approved the second-order kinetic yielding good R2 values of 0.997 and k values of 0.0301.
format Undergraduates Project Papers
author Nurfariha Fathmi, Zakaria
author_facet Nurfariha Fathmi, Zakaria
author_sort Nurfariha Fathmi, Zakaria
title Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol
title_short Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol
title_full Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol
title_fullStr Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol
title_full_unstemmed Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol
title_sort application of ultrasound technique for steviol glycosides separation from mixtures of s.rebaudiana stems and leaves using methanol
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7086/1/16.Application%20of%20ultrasound%20technique%20for%20Steviol%20Glycosides%20separation%20from%20mixtures%20of%20S.Rebaudiana%20stems%20and%20leaves%20using%20methanol.pdf
http://umpir.ump.edu.my/id/eprint/7086/
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score 13.211869

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