Antioxidant Activity Of Ethanolic Extract Of Some Malaysian Herbs
Antioxidant, usually found in food has the ability to prevent the oxidative damage to body cell by trapping free radicals, a highly unstable molecule. In this research, the antioxidant activity of extract from three selected Malaysian herbs was investigated. The herbs were Euodia redlevi (Tenggek Bu...
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/6967/1/21.Antioxidant%20activity%20of%20ethanolic%20extract%20of%20some%20malaysian%20herbs.pdf http://umpir.ump.edu.my/id/eprint/6967/ |
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| Summary: | Antioxidant, usually found in food has the ability to prevent the oxidative damage to body cell by trapping free radicals, a highly unstable molecule. In this research, the antioxidant activity of extract from three selected Malaysian herbs was investigated. The herbs were Euodia redlevi (Tenggek Burung), Cosmos caudatus (Ulam Raja) and Premma cordiflora (Bebuas). Besides, the Vitamin C content in the herbs was also determined. The herbs were collected from Tanjung Sedili (Kota Tinggi, Johor) and Kuantan (Pahang). The herbs were extracted using sonication extraction method at different interval of times (30, 60, 90 and 120 minutes) and at different concentrations of solvent (50%, 70% and 90% of ethanol). After the extraction, the extracts were analyzed for total phenolic content using Folin-Ciocalteu’s reagent and antioxidant activity using DPPH assay. The results show that the three herbs exhibited significant value of ascorbic acid content. The highest amount of ascorbic acid was obtained in Cosmos caudatus in 60 minutes of extraction using 70% of ethanol. As for Premma cordiflora and Euodia redlevi, the highest amount of ascorbic acid were obtained in 90 minutes of extraction using 90% of ethanol. The herbs extracts of the said parameters were analyzed again for their ascorbic acid or vitamin C content using High Performance Liquid Chromatography (HPLC). The results showed that Cosmos caudatus has the highest vitamin C content, followed by Premma cordiflora and finally Euodia redlevi. This research provides the information on the promising source of natural antioxidants in Malaysia. |
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