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Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide

Hepatitis B (Hep B) is a viral infection caused by hepatitis B virus (HBV) that infects the human liver. HBV is bloodborne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide. Approximately 5% of the Malaysian population is infected by HBV....

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Main Authors: Geraldine Chan, Sue Ching, Willy Wong, Xiu Fa, Chong, Fui Chin
Format: Article
Language:English
English
English
Published: Penerbit Universiti Teknologi Malaysia 2015
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TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/6745/1/fkksa-2014-Geraldine-Immobilization_Of_Hepatitis_B.pdf
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spelling my.ump.umpir.67452019-10-17T08:01:49Z http://umpir.ump.edu.my/id/eprint/6745/ Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide Geraldine Chan, Sue Ching Willy Wong, Xiu Fa Chong, Fui Chin TP Chemical technology Hepatitis B (Hep B) is a viral infection caused by hepatitis B virus (HBV) that infects the human liver. HBV is bloodborne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide. Approximately 5% of the Malaysian population is infected by HBV. The treatment to this disease is possible if early detection is done before irreversible liver damage occurs. The presence of Hepatitis B virus surface antigen (HB5Ag) is an evidence of the presence of HBV infections. Presently, the conventional methods used for detection shows weakness in terms of time and efficiency. In this research, we aim to study on the feasibility of reduced graphene oxide (rGO) as the base material for immune biosensor for the detection of HB5Ag. The graphene oxide was prepared via Modified Hummer's Method due to its ability to produce graphene in large quantity. The fabrication of immunosensor was conducted by immobilizing antibody onto the graphene. Nafion-graphene nanosheet was grown and later treated with Thionine solution. Following that, a solution of primary antibodies against Hep B surface antigen (anti-HB5Ag lgG) was incubated on the biosensor. After the immobilization, the antigen was dropped onto the biosensor, followed by incubation of secondary antibodies conjugated to Horseradish Peroxidase (HRP). Upon addition of Tetramethylbenzid me (TMB), the colour of the solution changes to blue. Penerbit Universiti Teknologi Malaysia 2015 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/6745/1/fkksa-2014-Geraldine-Immobilization_Of_Hepatitis_B.pdf pdf en http://umpir.ump.edu.my/id/eprint/6745/2/fkksa-2014-geraldine-posterbiojohor.JPG pdf en http://umpir.ump.edu.my/id/eprint/6745/8/fkksa-2015-geraldine-%20Immobilization%20of%20Hepatitis%20B%20Antibody.pdf Geraldine Chan, Sue Ching and Willy Wong, Xiu Fa and Chong, Fui Chin (2015) Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide. Jurnal Teknologi (Sciences and Engineering), 77 (31). pp. 25-29. ISSN 0127-9696 (print); 2180-3722 (online) https://doi.org/10.11113/jt.v77.6895 doi: 10.11113/jt.v77.6895
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Geraldine Chan, Sue Ching
Willy Wong, Xiu Fa
Chong, Fui Chin
Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
description Hepatitis B (Hep B) is a viral infection caused by hepatitis B virus (HBV) that infects the human liver. HBV is bloodborne and transfusion-transmitted human pathogen that has a large impact on blood safety and public health worldwide. Approximately 5% of the Malaysian population is infected by HBV. The treatment to this disease is possible if early detection is done before irreversible liver damage occurs. The presence of Hepatitis B virus surface antigen (HB5Ag) is an evidence of the presence of HBV infections. Presently, the conventional methods used for detection shows weakness in terms of time and efficiency. In this research, we aim to study on the feasibility of reduced graphene oxide (rGO) as the base material for immune biosensor for the detection of HB5Ag. The graphene oxide was prepared via Modified Hummer's Method due to its ability to produce graphene in large quantity. The fabrication of immunosensor was conducted by immobilizing antibody onto the graphene. Nafion-graphene nanosheet was grown and later treated with Thionine solution. Following that, a solution of primary antibodies against Hep B surface antigen (anti-HB5Ag lgG) was incubated on the biosensor. After the immobilization, the antigen was dropped onto the biosensor, followed by incubation of secondary antibodies conjugated to Horseradish Peroxidase (HRP). Upon addition of Tetramethylbenzid me (TMB), the colour of the solution changes to blue.
format Article
author Geraldine Chan, Sue Ching
Willy Wong, Xiu Fa
Chong, Fui Chin
author_facet Geraldine Chan, Sue Ching
Willy Wong, Xiu Fa
Chong, Fui Chin
author_sort Geraldine Chan, Sue Ching
title Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_short Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_full Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_fullStr Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_full_unstemmed Immobilization of Hepatitis B Antibody Onto the Reduced Graphene Oxide
title_sort immobilization of hepatitis b antibody onto the reduced graphene oxide
publisher Penerbit Universiti Teknologi Malaysia
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/6745/1/fkksa-2014-Geraldine-Immobilization_Of_Hepatitis_B.pdf
http://umpir.ump.edu.my/id/eprint/6745/2/fkksa-2014-geraldine-posterbiojohor.JPG
http://umpir.ump.edu.my/id/eprint/6745/8/fkksa-2015-geraldine-%20Immobilization%20of%20Hepatitis%20B%20Antibody.pdf
http://umpir.ump.edu.my/id/eprint/6745/
https://doi.org/10.11113/jt.v77.6895
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score 13.211869

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