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Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters

Xylose-rich sawdust hydrolysate can be an economic substrate for the enzymatic production of xylitol, a specialty product. It is important to identify the process factors influencing xylitol production. This research aimed to screen the parameters significantly affecting bioxylitol synthesis from wo...

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Main Authors: M. Rafiqul, Islam, Mimi Sakinah, A. M., Zularisam, A. W.
Format: Article
Language:English
Published: Springer 2015
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/6191/1/Enzymatic%20ProEnduction%20of%20Bioxylitol%20from%20Sawdust%20Hydrolysate-%20Screening%20of%20Process%20Parameters.pdf
http://umpir.ump.edu.my/id/eprint/6191/
http://dx.doi.org/10.1007/s12010-015-1630-2
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spelling my.ump.umpir.61912018-11-29T07:25:30Z http://umpir.ump.edu.my/id/eprint/6191/ Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters M. Rafiqul, Islam Mimi Sakinah, A. M. Zularisam, A. W. TP Chemical technology Xylose-rich sawdust hydrolysate can be an economic substrate for the enzymatic production of xylitol, a specialty product. It is important to identify the process factors influencing xylitol production. This research aimed to screen the parameters significantly affecting bioxylitol synthesis from wood sawdust by xylose reductase (XR). Enzymatic bioxylitol production was conducted to estimate the effect of different variables reaction time (2–18 h), temperature (20–70 °C), pH (4.0–9.0), NADPH (1.17–5.32 g/L), and enzyme concentration (2–6 %) on the yield of xylitol. Fractional factorial design was followed to identify the key process factors. The screening design identified that time, temperature, and pH are the most significant factors influencing bioxylitol production among the variables with the values of 12 h, 35 °C, and 7.0, respectively. These conditions led to a xylitol yield of 71 % (w/w). This is the first report on the statistical screening of process variables influencing enzyme-based bioxylitol production from lignocellulosic biomass. Springer 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6191/1/Enzymatic%20ProEnduction%20of%20Bioxylitol%20from%20Sawdust%20Hydrolysate-%20Screening%20of%20Process%20Parameters.pdf M. Rafiqul, Islam and Mimi Sakinah, A. M. and Zularisam, A. W. (2015) Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters. Applied Biochemistry and Biotechnology, 176 (4). pp. 1071-1083. ISSN 1559-0291 http://dx.doi.org/10.1007/s12010-015-1630-2 doi: 10.1007/s12010-015-1630-2
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
M. Rafiqul, Islam
Mimi Sakinah, A. M.
Zularisam, A. W.
Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters
description Xylose-rich sawdust hydrolysate can be an economic substrate for the enzymatic production of xylitol, a specialty product. It is important to identify the process factors influencing xylitol production. This research aimed to screen the parameters significantly affecting bioxylitol synthesis from wood sawdust by xylose reductase (XR). Enzymatic bioxylitol production was conducted to estimate the effect of different variables reaction time (2–18 h), temperature (20–70 °C), pH (4.0–9.0), NADPH (1.17–5.32 g/L), and enzyme concentration (2–6 %) on the yield of xylitol. Fractional factorial design was followed to identify the key process factors. The screening design identified that time, temperature, and pH are the most significant factors influencing bioxylitol production among the variables with the values of 12 h, 35 °C, and 7.0, respectively. These conditions led to a xylitol yield of 71 % (w/w). This is the first report on the statistical screening of process variables influencing enzyme-based bioxylitol production from lignocellulosic biomass.
format Article
author M. Rafiqul, Islam
Mimi Sakinah, A. M.
Zularisam, A. W.
author_facet M. Rafiqul, Islam
Mimi Sakinah, A. M.
Zularisam, A. W.
author_sort M. Rafiqul, Islam
title Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters
title_short Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters
title_full Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters
title_fullStr Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters
title_full_unstemmed Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters
title_sort enzymatic production of bioxylitol from sawdust hydrolysate: screening of process parameters
publisher Springer
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/6191/1/Enzymatic%20ProEnduction%20of%20Bioxylitol%20from%20Sawdust%20Hydrolysate-%20Screening%20of%20Process%20Parameters.pdf
http://umpir.ump.edu.my/id/eprint/6191/
http://dx.doi.org/10.1007/s12010-015-1630-2
_version_ 1643665324985286656
score 13.211869

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