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Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis

The purpose of this study is to investigate the effect of pH and enzyme loading on the concentration of protein during enzymatic hydrolysis of Collocalia Fuciphaga.C. Fuciphaga is refers to the bird nest from one swiftlet species that is used as the sample known to be highly nutritious.Bovine Serum...

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Main Author: Ahmad Afifi, Ibrahim
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/5843/1/CD7066.pdf
http://umpir.ump.edu.my/id/eprint/5843/
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spelling my.ump.umpir.58432021-06-09T03:00:58Z http://umpir.ump.edu.my/id/eprint/5843/ Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis Ahmad Afifi, Ibrahim TP Chemical technology The purpose of this study is to investigate the effect of pH and enzyme loading on the concentration of protein during enzymatic hydrolysis of Collocalia Fuciphaga.C. Fuciphaga is refers to the bird nest from one swiftlet species that is used as the sample known to be highly nutritious.Bovine Serum Albumin(BSA),a standard protein solution was used as the benchmark for the reference of the protein concentration.A research had been proposed in this study to optimize the production of protein from C.Fuciphaga. The effect of changing of the parameters values during the enzymatic hydrolysis was studied and the higher protein concentrations produced were highlighted.The standard protein curve of Bovine Serum Albumin(BSA)is prepared first with several dilutions for protein standard curve.The results from two significant parameters in the experiment was taken and analyzed.The selection of parameters values was taken from the enzymes’optimum condition where the pH value of Alcalase 2.4L is from pH 7 until pH 10 whiles the temperature is fixed at 50oC.Enzyme loading was varies from 1.5% to 3.0% (v/w).The sample was analyzed using UV-Vis Spectrophotometer to determine the concentration of protein in each sample.The optimum condition is found to be at extraction condition of pH 8.5 and enzyme concentration of 3.0% (v/w).Characterization of the sample had been done and validated the protein composition on the extracted sample. 2013-02 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5843/1/CD7066.pdf Ahmad Afifi, Ibrahim (2013) Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Ahmad Afifi, Ibrahim
Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis
description The purpose of this study is to investigate the effect of pH and enzyme loading on the concentration of protein during enzymatic hydrolysis of Collocalia Fuciphaga.C. Fuciphaga is refers to the bird nest from one swiftlet species that is used as the sample known to be highly nutritious.Bovine Serum Albumin(BSA),a standard protein solution was used as the benchmark for the reference of the protein concentration.A research had been proposed in this study to optimize the production of protein from C.Fuciphaga. The effect of changing of the parameters values during the enzymatic hydrolysis was studied and the higher protein concentrations produced were highlighted.The standard protein curve of Bovine Serum Albumin(BSA)is prepared first with several dilutions for protein standard curve.The results from two significant parameters in the experiment was taken and analyzed.The selection of parameters values was taken from the enzymes’optimum condition where the pH value of Alcalase 2.4L is from pH 7 until pH 10 whiles the temperature is fixed at 50oC.Enzyme loading was varies from 1.5% to 3.0% (v/w).The sample was analyzed using UV-Vis Spectrophotometer to determine the concentration of protein in each sample.The optimum condition is found to be at extraction condition of pH 8.5 and enzyme concentration of 3.0% (v/w).Characterization of the sample had been done and validated the protein composition on the extracted sample.
format Undergraduates Project Papers
author Ahmad Afifi, Ibrahim
author_facet Ahmad Afifi, Ibrahim
author_sort Ahmad Afifi, Ibrahim
title Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis
title_short Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis
title_full Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis
title_fullStr Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis
title_full_unstemmed Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis
title_sort effect of ph and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/5843/1/CD7066.pdf
http://umpir.ump.edu.my/id/eprint/5843/
_version_ 1702170042435633152
score 13.211869

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