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Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods

In this present study the antioxidant activity of two edible seaweeds Eucheuma cottonii and Padina sp. were determined. The two extraction methods such as conventional and soxhlet were used to extract secondary metabolites by using methanol as a solvent medium. Total phenolic content of crude seawee...

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Main Authors: Tam, Siow Foon, Lian, Ai Ai, Kuppusamy, Palaniselvam, M. M., Yusoff, Natanamurugaraj, Govindan
Format: Article
Language:English
Published: Medwell Publishing 2014
Subjects:
QD Chemistry
Online Access:http://umpir.ump.edu.my/id/eprint/5818/1/141-146.pdf
http://umpir.ump.edu.my/id/eprint/5818/
http://medwelljournals.com/abstract/?doi=rjasci.2014.141.146
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spelling my.ump.umpir.58182018-01-22T01:07:20Z http://umpir.ump.edu.my/id/eprint/5818/ Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods Tam, Siow Foon Lian, Ai Ai Kuppusamy, Palaniselvam M. M., Yusoff Natanamurugaraj, Govindan QD Chemistry In this present study the antioxidant activity of two edible seaweeds Eucheuma cottonii and Padina sp. were determined. The two extraction methods such as conventional and soxhlet were used to extract secondary metabolites by using methanol as a solvent medium. Total phenolic content of crude seaweed extracts were analysed by standard method. The different antioxidant assays 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching assays were studied. The DPPH and FRAP assays showed positive correlation with expressed higher total phenolic content of methanolic seaweed extracts. Also, β-carotene bleaching assay lower activity compare with BHT as reference control. IR spectra showed the phenolic related functional groups are present the seaweed extract. It mainly responsible for higher rate of antioxidant activity. The methanolic extracts of Padina sp. showed better radical scavenging and higher phenolic content than Eucheuma cottonii. Also, the soxhlet extraction showed higher yield and better radical scavenging activity compared to conventional method. Moreover, the studies confirmed both seaweeds are an effective candidate for the control the free radical scavenging activity. Medwell Publishing 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5818/1/141-146.pdf Tam, Siow Foon and Lian, Ai Ai and Kuppusamy, Palaniselvam and M. M., Yusoff and Natanamurugaraj, Govindan (2014) Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods. Research Journal of Applied Sciences, 9 (3). pp. 141-146. ISSN 1815-932X http://medwelljournals.com/abstract/?doi=rjasci.2014.141.146 10.3923/rjasci.2014.141.146
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Tam, Siow Foon
Lian, Ai Ai
Kuppusamy, Palaniselvam
M. M., Yusoff
Natanamurugaraj, Govindan
Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods
description In this present study the antioxidant activity of two edible seaweeds Eucheuma cottonii and Padina sp. were determined. The two extraction methods such as conventional and soxhlet were used to extract secondary metabolites by using methanol as a solvent medium. Total phenolic content of crude seaweed extracts were analysed by standard method. The different antioxidant assays 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching assays were studied. The DPPH and FRAP assays showed positive correlation with expressed higher total phenolic content of methanolic seaweed extracts. Also, β-carotene bleaching assay lower activity compare with BHT as reference control. IR spectra showed the phenolic related functional groups are present the seaweed extract. It mainly responsible for higher rate of antioxidant activity. The methanolic extracts of Padina sp. showed better radical scavenging and higher phenolic content than Eucheuma cottonii. Also, the soxhlet extraction showed higher yield and better radical scavenging activity compared to conventional method. Moreover, the studies confirmed both seaweeds are an effective candidate for the control the free radical scavenging activity.
format Article
author Tam, Siow Foon
Lian, Ai Ai
Kuppusamy, Palaniselvam
M. M., Yusoff
Natanamurugaraj, Govindan
author_facet Tam, Siow Foon
Lian, Ai Ai
Kuppusamy, Palaniselvam
M. M., Yusoff
Natanamurugaraj, Govindan
author_sort Tam, Siow Foon
title Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods
title_short Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods
title_full Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods
title_fullStr Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods
title_full_unstemmed Studies on in vitro Antioxidant Activity of Marine Edible Seaweed From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods
title_sort studies on in vitro antioxidant activity of marine edible seaweed from east coastal region, peninsular malaysia using different extraction methods
publisher Medwell Publishing
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/5818/1/141-146.pdf
http://umpir.ump.edu.my/id/eprint/5818/
http://medwelljournals.com/abstract/?doi=rjasci.2014.141.146
_version_ 1643665255275954176
score 13.211869

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