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Mixtures of S.rebaudiana Leaves and Stems Waste for Steviol g. (Steviosides) Synthesis Using Ethanol-Water Solvents

The s.rebaudiana leaves is used for steviol g. (steviosides) synthesis, but its stems were discarded without processing for added value products, thus this work aim to defeat this issues by using the mixtures of s.rebaudiana leaves and stems waste as an alternative feed stock for steviosides. The s...

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Main Authors: Said, N., Zulkarnain, Jema'at, Nurfariha F., Zakaria, Ahmad Z., Sulaiman
Format: Conference or Workshop Item
Language:English
Published: 2013
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/5693/1/CHEM-006.pdf
http://umpir.ump.edu.my/id/eprint/5693/
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spelling my.ump.umpir.56932018-05-21T07:33:05Z http://umpir.ump.edu.my/id/eprint/5693/ Mixtures of S.rebaudiana Leaves and Stems Waste for Steviol g. (Steviosides) Synthesis Using Ethanol-Water Solvents Said, N. Zulkarnain, Jema'at Nurfariha F., Zakaria Ahmad Z., Sulaiman TP Chemical technology The s.rebaudiana leaves is used for steviol g. (steviosides) synthesis, but its stems were discarded without processing for added value products, thus this work aim to defeat this issues by using the mixtures of s.rebaudiana leaves and stems waste as an alternative feed stock for steviosides. The steviosides are originating from S. rebaudiana plants which has up 300 times sweetener than sugar from sugar cane (1), and better in term of taste quality due to less bitter than reboudiosides A (2,3,4). Among 150 stevia species, the only one with significant sweet tasting properties is S. rebaudiana (7,8). The stevia extracts may benefit people at risk of diabetes, as they may help to control blood glucose level (9,10). The steviosides synthesis was conducted by ultrasound-assisted extraction (UAE)using ethanol-water as combination of polarity solvents. The mixture of S. rebaudiana stems and leaves were dried in open shade then milled to 60 mesh powder using grinder. Then, a sample of 500mg of the mixture of S. rebaudiana stems and leaves powder was sonicated with 50 mL of different percentage of ethanol in water (80:20, 60:40,40:60,20:80,100:0, v/v) in an ultrasonicator bath at various temperature from 200C – 500C for time of 10 minutes to 50 minutes. After extraction, the supernatant was filtered through microfiber filter disk using vacuum pump filter. The combined percolations were evaporated to dryness on a rotary evaporator at 550C and dissolved in water. Then the sample were analyzed by High Performance Liquid Chromatography (HPLC) using Eurospher 100 – 5 NH 2 column at flow rate of 1mL/m3 and temperature of 350C. Next, the 2.5 mg of the standard solution were prepared by diluting with 80% ethanol in water into volumetric flask respectively in prior to sonicate them for 15 minutes. Beside that, standard solutions were prepared before running the HPLC. The prepared solutions were diluted by the stock solution of 0.25 mg/mL into a 10 ml volumetric flask for concentration ranging from 0.001 to 0. It was found, the mixtures of s.rebaudiana leaves and stems has been successfully synthesized for steviosides. The synthesis process has been affected by the variation of parameters such as time, temperature, and ratio of solvent to water. The maximum yield of 2.81 % was resulted by the time of 30 min, temperature of 40oC and ratio of ethanol-water of 40 : 10 (v/v) or 80 : 20 (v/v). The kinetics of steviosides synthesisapproved the second-order kinetic yielding good R 2 values from of 0.997 and k values of 0.031. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5693/1/CHEM-006.pdf Said, N. and Zulkarnain, Jema'at and Nurfariha F., Zakaria and Ahmad Z., Sulaiman (2013) Mixtures of S.rebaudiana Leaves and Stems Waste for Steviol g. (Steviosides) Synthesis Using Ethanol-Water Solvents. In: Malaysian Technical Universities Conference on Engineering & Technology (MUCET 2013), 3-4 December 2013 , Kuantan, Pahang. pp. 1-3..
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Said, N.
Zulkarnain, Jema'at
Nurfariha F., Zakaria
Ahmad Z., Sulaiman
Mixtures of S.rebaudiana Leaves and Stems Waste for Steviol g. (Steviosides) Synthesis Using Ethanol-Water Solvents
description The s.rebaudiana leaves is used for steviol g. (steviosides) synthesis, but its stems were discarded without processing for added value products, thus this work aim to defeat this issues by using the mixtures of s.rebaudiana leaves and stems waste as an alternative feed stock for steviosides. The steviosides are originating from S. rebaudiana plants which has up 300 times sweetener than sugar from sugar cane (1), and better in term of taste quality due to less bitter than reboudiosides A (2,3,4). Among 150 stevia species, the only one with significant sweet tasting properties is S. rebaudiana (7,8). The stevia extracts may benefit people at risk of diabetes, as they may help to control blood glucose level (9,10). The steviosides synthesis was conducted by ultrasound-assisted extraction (UAE)using ethanol-water as combination of polarity solvents. The mixture of S. rebaudiana stems and leaves were dried in open shade then milled to 60 mesh powder using grinder. Then, a sample of 500mg of the mixture of S. rebaudiana stems and leaves powder was sonicated with 50 mL of different percentage of ethanol in water (80:20, 60:40,40:60,20:80,100:0, v/v) in an ultrasonicator bath at various temperature from 200C – 500C for time of 10 minutes to 50 minutes. After extraction, the supernatant was filtered through microfiber filter disk using vacuum pump filter. The combined percolations were evaporated to dryness on a rotary evaporator at 550C and dissolved in water. Then the sample were analyzed by High Performance Liquid Chromatography (HPLC) using Eurospher 100 – 5 NH 2 column at flow rate of 1mL/m3 and temperature of 350C. Next, the 2.5 mg of the standard solution were prepared by diluting with 80% ethanol in water into volumetric flask respectively in prior to sonicate them for 15 minutes. Beside that, standard solutions were prepared before running the HPLC. The prepared solutions were diluted by the stock solution of 0.25 mg/mL into a 10 ml volumetric flask for concentration ranging from 0.001 to 0. It was found, the mixtures of s.rebaudiana leaves and stems has been successfully synthesized for steviosides. The synthesis process has been affected by the variation of parameters such as time, temperature, and ratio of solvent to water. The maximum yield of 2.81 % was resulted by the time of 30 min, temperature of 40oC and ratio of ethanol-water of 40 : 10 (v/v) or 80 : 20 (v/v). The kinetics of steviosides synthesisapproved the second-order kinetic yielding good R 2 values from of 0.997 and k values of 0.031.
format Conference or Workshop Item
author Said, N.
Zulkarnain, Jema'at
Nurfariha F., Zakaria
Ahmad Z., Sulaiman
author_facet Said, N.
Zulkarnain, Jema'at
Nurfariha F., Zakaria
Ahmad Z., Sulaiman
author_sort Said, N.
title Mixtures of S.rebaudiana Leaves and Stems Waste for Steviol g. (Steviosides) Synthesis Using Ethanol-Water Solvents
title_short Mixtures of S.rebaudiana Leaves and Stems Waste for Steviol g. (Steviosides) Synthesis Using Ethanol-Water Solvents
title_full Mixtures of S.rebaudiana Leaves and Stems Waste for Steviol g. (Steviosides) Synthesis Using Ethanol-Water Solvents
title_fullStr Mixtures of S.rebaudiana Leaves and Stems Waste for Steviol g. (Steviosides) Synthesis Using Ethanol-Water Solvents
title_full_unstemmed Mixtures of S.rebaudiana Leaves and Stems Waste for Steviol g. (Steviosides) Synthesis Using Ethanol-Water Solvents
title_sort mixtures of s.rebaudiana leaves and stems waste for steviol g. (steviosides) synthesis using ethanol-water solvents
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/5693/1/CHEM-006.pdf
http://umpir.ump.edu.my/id/eprint/5693/
_version_ 1643665236395294720
score 13.211869

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