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Biotransformation of phenol using immobilized polyphenol oxidase from banana stem

Polyphenol oxidase(PPO) is an enzyme that can easily find in most of the plants.It is a browning agent that cause from catalyzing the oxidation of various phenolic compounds to the corresponding quinones which lead to the formation of melanin pigment.PPO commonly used as biocatalyst in a biotransfor...

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Main Author: Azhari, Anuar
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/4617/1/02.Biotransformation%20of%20phenol%20using%20immobilized%20polyphenol%20oxidase%20from%20banana%20stem.pdf
http://umpir.ump.edu.my/id/eprint/4617/
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spelling my.ump.umpir.46172023-06-26T07:09:41Z http://umpir.ump.edu.my/id/eprint/4617/ Biotransformation of phenol using immobilized polyphenol oxidase from banana stem Azhari, Anuar TP Chemical technology Polyphenol oxidase(PPO) is an enzyme that can easily find in most of the plants.It is a browning agent that cause from catalyzing the oxidation of various phenolic compounds to the corresponding quinones which lead to the formation of melanin pigment.PPO commonly used as biocatalyst in a biotransformation reaction and in this study is converting phenol to less harmful compound.The objective of this study was to study the basic kinetics of batch and continuous reactors.By conducting biotransformation using polyphenol oxidase extracted from banana stem. The enzyme immobilized using Ca-Alginate in the forms of beads.Five different phenol concentrations were transformed using different enzyme loadings for batch mode while different flow rates were used in continuous mode.Samples were analyzed using High Performance Liquid Chromatography(HPLC)at 254nm,using methanol and water (20:80) as mobile phase at 1mL/min.The space time of a batch reactor was determined and weight of catalyst loading was estimated for continuous reactor. 2010-04 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/4617/1/02.Biotransformation%20of%20phenol%20using%20immobilized%20polyphenol%20oxidase%20from%20banana%20stem.pdf Azhari, Anuar (2010) Biotransformation of phenol using immobilized polyphenol oxidase from banana stem. Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Azhari, Anuar
Biotransformation of phenol using immobilized polyphenol oxidase from banana stem
description Polyphenol oxidase(PPO) is an enzyme that can easily find in most of the plants.It is a browning agent that cause from catalyzing the oxidation of various phenolic compounds to the corresponding quinones which lead to the formation of melanin pigment.PPO commonly used as biocatalyst in a biotransformation reaction and in this study is converting phenol to less harmful compound.The objective of this study was to study the basic kinetics of batch and continuous reactors.By conducting biotransformation using polyphenol oxidase extracted from banana stem. The enzyme immobilized using Ca-Alginate in the forms of beads.Five different phenol concentrations were transformed using different enzyme loadings for batch mode while different flow rates were used in continuous mode.Samples were analyzed using High Performance Liquid Chromatography(HPLC)at 254nm,using methanol and water (20:80) as mobile phase at 1mL/min.The space time of a batch reactor was determined and weight of catalyst loading was estimated for continuous reactor.
format Undergraduates Project Papers
author Azhari, Anuar
author_facet Azhari, Anuar
author_sort Azhari, Anuar
title Biotransformation of phenol using immobilized polyphenol oxidase from banana stem
title_short Biotransformation of phenol using immobilized polyphenol oxidase from banana stem
title_full Biotransformation of phenol using immobilized polyphenol oxidase from banana stem
title_fullStr Biotransformation of phenol using immobilized polyphenol oxidase from banana stem
title_full_unstemmed Biotransformation of phenol using immobilized polyphenol oxidase from banana stem
title_sort biotransformation of phenol using immobilized polyphenol oxidase from banana stem
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/4617/1/02.Biotransformation%20of%20phenol%20using%20immobilized%20polyphenol%20oxidase%20from%20banana%20stem.pdf
http://umpir.ump.edu.my/id/eprint/4617/
_version_ 1769842512182116352
score 13.211869

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