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Optimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM)

Acetobacter xylinum is a type of acetic acid producing bacteria that can synthesis bacterial cellulose from carbohydrates.Bacterial cellulose that produced has high purity,high water holding capacity,good mechanical strength,elasticity,high crystallinity and high porosity compare to plant cellulose....

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Main Author: Abu Hassan, Mohd Nazir
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/4524/1/CD6481_ABU_HASSAN_MOHD_NAZIR.pdf
http://umpir.ump.edu.my/id/eprint/4524/
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spelling my.ump.umpir.45242021-07-16T04:03:29Z http://umpir.ump.edu.my/id/eprint/4524/ Optimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM) Abu Hassan, Mohd Nazir TP Chemical technology Acetobacter xylinum is a type of acetic acid producing bacteria that can synthesis bacterial cellulose from carbohydrates.Bacterial cellulose that produced has high purity,high water holding capacity,good mechanical strength,elasticity,high crystallinity and high porosity compare to plant cellulose.This research was using apple juice as the high potential carbon sources to replace the pure carbon sources as the substrate for the synthesis of bacterial cellulose.The objective of this study was to optimize bacterial cellulose production in apple juice medium by using Response Surface Methodology (RSM).The research will be conducted by using 5 samples with difference temperature (28ºC, 29ºC, 30ºC, 31ºC and 32ºC) , 5 sample with difference in pH (4, 5, 6 ,7 and 8) and 5 sample with different medium concentration (60 %, 70%, 80%,90% and 100%).Each sample contains 100mL of medium in 250mL conical flask and incubated in incubator for 5 days.The bacterial cellulose film produced by Acetobacter Xylinum was treated with 1% Natrium Hydroxide (NaOH) for 1 day and then washed with Deionized water to neutralize the bacterial cellulose. The result showed that the medium concentration,pH and temperature of cultivation were affected the production yield of bacterial cellulose.By using response surface methodology (RSM),the optimum condition for bacterial cellulose production was 95% (v/v) for medium concentration, pH 5.95 and cultivation temperature at 30.3ºC.The film then was analyzed by using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). By using FTIR the hydrogen bonds (-OH) of bacterial cellulose was determined while by using SEM the interwoven strands,ribbon-like (microfibrils) of bacterial cellulose structure was observed.Thus,the optimum conditions for bacterial cellulose production can be determined by using RSM. 2012-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/4524/1/CD6481_ABU_HASSAN_MOHD_NAZIR.pdf Abu Hassan, Mohd Nazir (2012) Optimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM). Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Abu Hassan, Mohd Nazir
Optimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM)
description Acetobacter xylinum is a type of acetic acid producing bacteria that can synthesis bacterial cellulose from carbohydrates.Bacterial cellulose that produced has high purity,high water holding capacity,good mechanical strength,elasticity,high crystallinity and high porosity compare to plant cellulose.This research was using apple juice as the high potential carbon sources to replace the pure carbon sources as the substrate for the synthesis of bacterial cellulose.The objective of this study was to optimize bacterial cellulose production in apple juice medium by using Response Surface Methodology (RSM).The research will be conducted by using 5 samples with difference temperature (28ºC, 29ºC, 30ºC, 31ºC and 32ºC) , 5 sample with difference in pH (4, 5, 6 ,7 and 8) and 5 sample with different medium concentration (60 %, 70%, 80%,90% and 100%).Each sample contains 100mL of medium in 250mL conical flask and incubated in incubator for 5 days.The bacterial cellulose film produced by Acetobacter Xylinum was treated with 1% Natrium Hydroxide (NaOH) for 1 day and then washed with Deionized water to neutralize the bacterial cellulose. The result showed that the medium concentration,pH and temperature of cultivation were affected the production yield of bacterial cellulose.By using response surface methodology (RSM),the optimum condition for bacterial cellulose production was 95% (v/v) for medium concentration, pH 5.95 and cultivation temperature at 30.3ºC.The film then was analyzed by using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). By using FTIR the hydrogen bonds (-OH) of bacterial cellulose was determined while by using SEM the interwoven strands,ribbon-like (microfibrils) of bacterial cellulose structure was observed.Thus,the optimum conditions for bacterial cellulose production can be determined by using RSM.
format Undergraduates Project Papers
author Abu Hassan, Mohd Nazir
author_facet Abu Hassan, Mohd Nazir
author_sort Abu Hassan, Mohd Nazir
title Optimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM)
title_short Optimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM)
title_full Optimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM)
title_fullStr Optimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM)
title_full_unstemmed Optimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM)
title_sort optimization of bacterial cellulose production in apple juice medium by using response surface methodology (rsm)
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/4524/1/CD6481_ABU_HASSAN_MOHD_NAZIR.pdf
http://umpir.ump.edu.my/id/eprint/4524/
_version_ 1706957160427028480
score 13.211869

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