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Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist i...

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Main Authors: Silvanir, ., Lai, Sin Yuan, Azren Aida, Asmawi, Chew, Kit Wayne, Ngan, Cheng Loong
Format: Article
Language:English
English
Published: Elsevier 2024
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/42995/1/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp_ABST.pdf
http://umpir.ump.edu.my/id/eprint/42995/2/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp.pdf
http://umpir.ump.edu.my/id/eprint/42995/
https://doi.org/10.1016/j.biortech.2023.130094
https://doi.org/10.1016/j.biortech.2023.130094
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spelling my.ump.umpir.429952024-12-02T07:07:47Z http://umpir.ump.edu.my/id/eprint/42995/ Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp Silvanir, . Lai, Sin Yuan Azren Aida, Asmawi Chew, Kit Wayne Ngan, Cheng Loong TP Chemical technology Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min. Elsevier 2024-02 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/42995/1/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp_ABST.pdf pdf en http://umpir.ump.edu.my/id/eprint/42995/2/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp.pdf Silvanir, . and Lai, Sin Yuan and Azren Aida, Asmawi and Chew, Kit Wayne and Ngan, Cheng Loong (2024) Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp. Bioresource Technology, 393 (130094). pp. 1-8. ISSN 0960-8524. (Published) https://doi.org/10.1016/j.biortech.2023.130094 https://doi.org/10.1016/j.biortech.2023.130094
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Silvanir, .
Lai, Sin Yuan
Azren Aida, Asmawi
Chew, Kit Wayne
Ngan, Cheng Loong
Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp
description Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K3PO4 salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min.
format Article
author Silvanir, .
Lai, Sin Yuan
Azren Aida, Asmawi
Chew, Kit Wayne
Ngan, Cheng Loong
author_facet Silvanir, .
Lai, Sin Yuan
Azren Aida, Asmawi
Chew, Kit Wayne
Ngan, Cheng Loong
author_sort Silvanir, .
title Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp
title_short Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp
title_full Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp
title_fullStr Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp
title_full_unstemmed Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp
title_sort application of high shear-assisted liquid biphasic system for protein extraction from chlorella sp
publisher Elsevier
publishDate 2024
url http://umpir.ump.edu.my/id/eprint/42995/1/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp_ABST.pdf
http://umpir.ump.edu.my/id/eprint/42995/2/Application%20of%20high%20shear-assisted%20liquid%20biphasic%20system%20for%20protein%20extraction%20from%20Chlorella%20sp.pdf
http://umpir.ump.edu.my/id/eprint/42995/
https://doi.org/10.1016/j.biortech.2023.130094
https://doi.org/10.1016/j.biortech.2023.130094
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score 13.23648

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