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Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes

The burgeoning crises of antimicrobial resistance and plastic pollution are converging in healthcare settings, presenting a complex challenge to global health. This study investigates the microbial populations in healthcare waste to understand the extent of antimicrobial resistance and the potential...

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Main Authors: Siew, Shing Wei, Mohamad Hazwan Fikri, Khairi, Norhisham, Abdul Hamid, Mohd Fazli, Farida Asras, Hajar Fauzan, Ahmad
Format: Article
Language:English
English
English
Published: Elsevier 2024
Subjects:
Q Science (General)
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/42937/1/1-s2.0-S0269749124020475-main.pdf
http://umpir.ump.edu.my/id/eprint/42937/7/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria_ABST.pdf
http://umpir.ump.edu.my/id/eprint/42937/8/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria.pdf
http://umpir.ump.edu.my/id/eprint/42937/
https://doi.org/10.1016/j.envpol.2024.125330
https://doi.org/10.1016/j.envpol.2024.125330
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spelling my.ump.umpir.429372024-11-25T01:50:11Z http://umpir.ump.edu.my/id/eprint/42937/ Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes Siew, Shing Wei Mohamad Hazwan Fikri, Khairi Norhisham, Abdul Hamid Mohd Fazli, Farida Asras Hajar Fauzan, Ahmad Q Science (General) TP Chemical technology The burgeoning crises of antimicrobial resistance and plastic pollution are converging in healthcare settings, presenting a complex challenge to global health. This study investigates the microbial populations in healthcare waste to understand the extent of antimicrobial resistance and the potential for plastic degradation by bacteria. Our metagenomic analysis, using both amplicon and shallow shotgun sequencing, provided a comprehensive view of the taxonomic diversity and functional capacity of the microbial consortia. The viable bacteria in healthcare waste samples were analyzed employing full-length 16S rRNA sequencing, revealing a diverse bacterial community dominated by Firmicutes and Proteobacteria phyla. Notably, Proteus mirabilis VFC3/3 and Pseudomonas sp. VFA2/3 were detected, while Stenotrophomonas maltophilia VFV3/2 surfaced as the predominant species, holding implications for the spread of hospital-acquired infections and antimicrobial resistance. Antibiotic susceptibility testing identified multidrug-resistant strains conferring antimicrobial genes, including the broad-spectrum antibiotic carbapenem, underscoring the critical need for improved waste management and infection control measures. Remarkably, we found genes linked to the breakdown of plastic that encoded for enzymes of the esterase, depolymerase, and oxidoreductase classes. This suggests that specific bacteria found in medical waste may be able to reduce the amount of plastic pollution that comes from biological and medical waste. The information is helpful in formulating strategies to counter the combined problems of environmental pollution and antibiotic resistance. This study emphasises the importance of monitoring microbial communities in hospital waste in order to influence waste management procedures and public health policy. The findings highlight the need for a multidisciplinary approach to mitigate the risks associated with antimicrobial resistance and plastic waste, especially in hospital settings where they intersect most acutely. Elsevier 2024-11-16 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/42937/1/1-s2.0-S0269749124020475-main.pdf pdf en http://umpir.ump.edu.my/id/eprint/42937/7/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria_ABST.pdf pdf en http://umpir.ump.edu.my/id/eprint/42937/8/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria.pdf Siew, Shing Wei and Mohamad Hazwan Fikri, Khairi and Norhisham, Abdul Hamid and Mohd Fazli, Farida Asras and Hajar Fauzan, Ahmad (2024) Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes. Environmental Pollution, 364 (125330). pp. 1-11. ISSN 1873-6424. (In Press / Online First) (In Press / Online First) https://doi.org/10.1016/j.envpol.2024.125330 https://doi.org/10.1016/j.envpol.2024.125330
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
English
topic Q Science (General)
TP Chemical technology
spellingShingle Q Science (General)
TP Chemical technology
Siew, Shing Wei
Mohamad Hazwan Fikri, Khairi
Norhisham, Abdul Hamid
Mohd Fazli, Farida Asras
Hajar Fauzan, Ahmad
Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes
description The burgeoning crises of antimicrobial resistance and plastic pollution are converging in healthcare settings, presenting a complex challenge to global health. This study investigates the microbial populations in healthcare waste to understand the extent of antimicrobial resistance and the potential for plastic degradation by bacteria. Our metagenomic analysis, using both amplicon and shallow shotgun sequencing, provided a comprehensive view of the taxonomic diversity and functional capacity of the microbial consortia. The viable bacteria in healthcare waste samples were analyzed employing full-length 16S rRNA sequencing, revealing a diverse bacterial community dominated by Firmicutes and Proteobacteria phyla. Notably, Proteus mirabilis VFC3/3 and Pseudomonas sp. VFA2/3 were detected, while Stenotrophomonas maltophilia VFV3/2 surfaced as the predominant species, holding implications for the spread of hospital-acquired infections and antimicrobial resistance. Antibiotic susceptibility testing identified multidrug-resistant strains conferring antimicrobial genes, including the broad-spectrum antibiotic carbapenem, underscoring the critical need for improved waste management and infection control measures. Remarkably, we found genes linked to the breakdown of plastic that encoded for enzymes of the esterase, depolymerase, and oxidoreductase classes. This suggests that specific bacteria found in medical waste may be able to reduce the amount of plastic pollution that comes from biological and medical waste. The information is helpful in formulating strategies to counter the combined problems of environmental pollution and antibiotic resistance. This study emphasises the importance of monitoring microbial communities in hospital waste in order to influence waste management procedures and public health policy. The findings highlight the need for a multidisciplinary approach to mitigate the risks associated with antimicrobial resistance and plastic waste, especially in hospital settings where they intersect most acutely.
format Article
author Siew, Shing Wei
Mohamad Hazwan Fikri, Khairi
Norhisham, Abdul Hamid
Mohd Fazli, Farida Asras
Hajar Fauzan, Ahmad
author_facet Siew, Shing Wei
Mohamad Hazwan Fikri, Khairi
Norhisham, Abdul Hamid
Mohd Fazli, Farida Asras
Hajar Fauzan, Ahmad
author_sort Siew, Shing Wei
title Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes
title_short Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes
title_full Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes
title_fullStr Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes
title_full_unstemmed Shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes
title_sort shallow shotgun sequencing of healthcare waste reveals plastic-eating bacteria with broad-spectrum antibiotic resistance genes
publisher Elsevier
publishDate 2024
url http://umpir.ump.edu.my/id/eprint/42937/1/1-s2.0-S0269749124020475-main.pdf
http://umpir.ump.edu.my/id/eprint/42937/7/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria_ABST.pdf
http://umpir.ump.edu.my/id/eprint/42937/8/Shallow%20shotgun%20sequencing%20of%20healthcare%20waste%20reveals%20plastic-eating%20bacteria.pdf
http://umpir.ump.edu.my/id/eprint/42937/
https://doi.org/10.1016/j.envpol.2024.125330
https://doi.org/10.1016/j.envpol.2024.125330
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score 13.235796

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