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Harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building

The hospital indoor air microbiome, a diverse range of microorganisms, gains prominence amid the COVID-19 pandemic. Elevated awareness underscores implications for patient and staff well-being. Concerns about risks to indoor air quality persist due to prolonged indoor exposure, necessitating further...

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Main Authors: Nor Husna, Mat Hussin, Tay, Darren Dean, Ummu Afeera, Zainulabid, Mohd Norhafsam, Maghpor, Hajar Fauzan, Ahmad
Format: Article
Language:English
Published: Elsevier 2024
Subjects:
Q Science (General)
TD Environmental technology. Sanitary engineering
Online Access:http://umpir.ump.edu.my/id/eprint/42615/1/Harnessing%20next-generation%20sequencing%20to%20monitor%20unculturable%20pathogenic%20bacteria.pdf
http://umpir.ump.edu.my/id/eprint/42615/
https://doi.org/10.1016/j.microb.2024.100163
https://doi.org/10.1016/j.microb.2024.100163
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spelling my.ump.umpir.426152024-09-23T02:15:33Z http://umpir.ump.edu.my/id/eprint/42615/ Harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building Nor Husna, Mat Hussin Tay, Darren Dean Ummu Afeera, Zainulabid Mohd Norhafsam, Maghpor Hajar Fauzan, Ahmad Q Science (General) TD Environmental technology. Sanitary engineering The hospital indoor air microbiome, a diverse range of microorganisms, gains prominence amid the COVID-19 pandemic. Elevated awareness underscores implications for patient and staff well-being. Concerns about risks to indoor air quality persist due to prolonged indoor exposure, necessitating further research on specific threats within the hospital environment. In this study, an independent culture-based approach was used to analyze the baseline core microbiome present in hospital environments, utilizing amplicon sequencing on the next-generation sequencing technology to target the V3 region of the 16S rRNA gene. Firmicutes, Proteobacteria, and Actinobacteria were the main bacterial phyla that were most isolated from the wards and clinics with different orders of abundance; Firmicutes being associated more in clinics and Actinobacteriota in wards. The bacteria Niallia taxi, Methyloversatilis universalis, unclassified Rummeliibacillus, unclassified Clostridium, and unclassified Sphingomonadaceae dominated the clinic area while ward areas reported Pseudonocardia bannensis, Rubrobacter aplysinae, unclassified Brachybacterium, unclassified mBradyrhizobium, and unclassified Mycobacterium to be the top five features. While the alpha-diversity index showed no significant differences, the beta-diversity analysis showed a significant difference between clinic and ward areas (p<0.05). Certain bacterial species associated with opportunistic pathogens as well as normal skin flora such as Methylobacterium spp., Cutibacterium spp., unclassified Sphingomonadaceae, and Anoxybacillus B spp., were also identified across all samples. The methods described in this research aim to establish a rapid and sensitive screening process that could be valuable for disease surveillance within the healthcare setting, shedding light on the potential impacts of the hospital microbiome on human illness. Elsevier 2024-09-12 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/42615/1/Harnessing%20next-generation%20sequencing%20to%20monitor%20unculturable%20pathogenic%20bacteria.pdf Nor Husna, Mat Hussin and Tay, Darren Dean and Ummu Afeera, Zainulabid and Mohd Norhafsam, Maghpor and Hajar Fauzan, Ahmad (2024) Harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building. The Microbe, 4 (100163). pp. 1-10. ISSN 2950-1946. (Published) https://doi.org/10.1016/j.microb.2024.100163 https://doi.org/10.1016/j.microb.2024.100163
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
TD Environmental technology. Sanitary engineering
spellingShingle Q Science (General)
TD Environmental technology. Sanitary engineering
Nor Husna, Mat Hussin
Tay, Darren Dean
Ummu Afeera, Zainulabid
Mohd Norhafsam, Maghpor
Hajar Fauzan, Ahmad
Harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building
description The hospital indoor air microbiome, a diverse range of microorganisms, gains prominence amid the COVID-19 pandemic. Elevated awareness underscores implications for patient and staff well-being. Concerns about risks to indoor air quality persist due to prolonged indoor exposure, necessitating further research on specific threats within the hospital environment. In this study, an independent culture-based approach was used to analyze the baseline core microbiome present in hospital environments, utilizing amplicon sequencing on the next-generation sequencing technology to target the V3 region of the 16S rRNA gene. Firmicutes, Proteobacteria, and Actinobacteria were the main bacterial phyla that were most isolated from the wards and clinics with different orders of abundance; Firmicutes being associated more in clinics and Actinobacteriota in wards. The bacteria Niallia taxi, Methyloversatilis universalis, unclassified Rummeliibacillus, unclassified Clostridium, and unclassified Sphingomonadaceae dominated the clinic area while ward areas reported Pseudonocardia bannensis, Rubrobacter aplysinae, unclassified Brachybacterium, unclassified mBradyrhizobium, and unclassified Mycobacterium to be the top five features. While the alpha-diversity index showed no significant differences, the beta-diversity analysis showed a significant difference between clinic and ward areas (p<0.05). Certain bacterial species associated with opportunistic pathogens as well as normal skin flora such as Methylobacterium spp., Cutibacterium spp., unclassified Sphingomonadaceae, and Anoxybacillus B spp., were also identified across all samples. The methods described in this research aim to establish a rapid and sensitive screening process that could be valuable for disease surveillance within the healthcare setting, shedding light on the potential impacts of the hospital microbiome on human illness.
format Article
author Nor Husna, Mat Hussin
Tay, Darren Dean
Ummu Afeera, Zainulabid
Mohd Norhafsam, Maghpor
Hajar Fauzan, Ahmad
author_facet Nor Husna, Mat Hussin
Tay, Darren Dean
Ummu Afeera, Zainulabid
Mohd Norhafsam, Maghpor
Hajar Fauzan, Ahmad
author_sort Nor Husna, Mat Hussin
title Harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building
title_short Harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building
title_full Harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building
title_fullStr Harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building
title_full_unstemmed Harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building
title_sort harnessing next-generation sequencing to monitor unculturable pathogenic bacteria in the indoor hospital building
publisher Elsevier
publishDate 2024
url http://umpir.ump.edu.my/id/eprint/42615/1/Harnessing%20next-generation%20sequencing%20to%20monitor%20unculturable%20pathogenic%20bacteria.pdf
http://umpir.ump.edu.my/id/eprint/42615/
https://doi.org/10.1016/j.microb.2024.100163
https://doi.org/10.1016/j.microb.2024.100163
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score 13.235796

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