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Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments

Tongkat Ali, a common name used to identify at least three plants, Eurycoma longifolia, Polyathia bullata, and Stema tuberosa, is recognized for its aphrodisiac bioactive constituent attributed to glycoproteins, particularly in E. longifolia. This study explores the presence and bioactivity of glyco...

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Main Authors: Vejayan, Jaya, Fatinah, Ahmad, Sharifah, A.T. Said, Chakravarthi, Srikumar, Karikalan, Barani
Format: Article
Language:English
Published: Faculty of Pharmacy, University of Benin 2024
Subjects:
Q Science (General)
R Medicine (General)
SF Animal culture
Online Access:http://umpir.ump.edu.my/id/eprint/42501/1/Glycoproteins%20Isolated%20from%20Tongkat%20Ali%20Plants%20Capable%20t.pdf
http://umpir.ump.edu.my/id/eprint/42501/
https://doi.org/10.26538/tjnpr/v8i6.41
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spelling my.ump.umpir.425012024-09-06T07:17:16Z http://umpir.ump.edu.my/id/eprint/42501/ Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments Vejayan, Jaya Fatinah, Ahmad Sharifah, A.T. Said Chakravarthi, Srikumar Karikalan, Barani Q Science (General) R Medicine (General) SF Animal culture Tongkat Ali, a common name used to identify at least three plants, Eurycoma longifolia, Polyathia bullata, and Stema tuberosa, is recognized for its aphrodisiac bioactive constituent attributed to glycoproteins, particularly in E. longifolia. This study explores the presence and bioactivity of glycoproteins in P. bullata and S. tuberosa, employing similar extraction methods to E. longifolia. The crude extracts from all three plants were isolated, and glycoproteins were separated using lectin affinity chromatography. The glycoprotein yields were determined, and the protein characterization was conducted using Sodium Dodecyl Sulphate Gel Electrophoresis (SDS PAGE). Consequently, through both in vitro and in vivo experiments, the bioactivity of the isolated glycoproteins was tested for their ability to elevate testosterone levels. In vitro studies conducted on approximately 4000 TM-3 Leydig cells revealed that treatment with 50 µg/mL of glycoproteins extracted from P. bullata and S. tuberosa resulted in a notable increase in testosterone concentration by 45% and 48%, respectively, compared to the untreated cells after 72 h. Moreover, in groups of mice administered with the isolated glycoproteins for 20 days, testosterone concentrations increased by over 50% compared to the control groups. Despite the relatively low yields of glycoproteins, with only 0.26% and 0.21% present in the roots of P. bullata and S. tuberosa, respectively, their effectiveness was evident. This highlights that the aphrodisiac bioactive constituents, are glycoproteins, within all three Tongkat Ali plants. Faculty of Pharmacy, University of Benin 2024-07-01 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/42501/1/Glycoproteins%20Isolated%20from%20Tongkat%20Ali%20Plants%20Capable%20t.pdf Vejayan, Jaya and Fatinah, Ahmad and Sharifah, A.T. Said and Chakravarthi, Srikumar and Karikalan, Barani (2024) Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments. Tropical Journal of Natural Product Research, 8 (6). pp. 7303-7309. ISSN 2616-0684. (Published) https://doi.org/10.26538/tjnpr/v8i6.41 10.26538/tjnpr/v8i6.41
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
R Medicine (General)
SF Animal culture
spellingShingle Q Science (General)
R Medicine (General)
SF Animal culture
Vejayan, Jaya
Fatinah, Ahmad
Sharifah, A.T. Said
Chakravarthi, Srikumar
Karikalan, Barani
Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments
description Tongkat Ali, a common name used to identify at least three plants, Eurycoma longifolia, Polyathia bullata, and Stema tuberosa, is recognized for its aphrodisiac bioactive constituent attributed to glycoproteins, particularly in E. longifolia. This study explores the presence and bioactivity of glycoproteins in P. bullata and S. tuberosa, employing similar extraction methods to E. longifolia. The crude extracts from all three plants were isolated, and glycoproteins were separated using lectin affinity chromatography. The glycoprotein yields were determined, and the protein characterization was conducted using Sodium Dodecyl Sulphate Gel Electrophoresis (SDS PAGE). Consequently, through both in vitro and in vivo experiments, the bioactivity of the isolated glycoproteins was tested for their ability to elevate testosterone levels. In vitro studies conducted on approximately 4000 TM-3 Leydig cells revealed that treatment with 50 µg/mL of glycoproteins extracted from P. bullata and S. tuberosa resulted in a notable increase in testosterone concentration by 45% and 48%, respectively, compared to the untreated cells after 72 h. Moreover, in groups of mice administered with the isolated glycoproteins for 20 days, testosterone concentrations increased by over 50% compared to the control groups. Despite the relatively low yields of glycoproteins, with only 0.26% and 0.21% present in the roots of P. bullata and S. tuberosa, respectively, their effectiveness was evident. This highlights that the aphrodisiac bioactive constituents, are glycoproteins, within all three Tongkat Ali plants.
format Article
author Vejayan, Jaya
Fatinah, Ahmad
Sharifah, A.T. Said
Chakravarthi, Srikumar
Karikalan, Barani
author_facet Vejayan, Jaya
Fatinah, Ahmad
Sharifah, A.T. Said
Chakravarthi, Srikumar
Karikalan, Barani
author_sort Vejayan, Jaya
title Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments
title_short Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments
title_full Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments
title_fullStr Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments
title_full_unstemmed Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments
title_sort glycoproteins isolated from tongkat ali plants capable to elevate testosterone in both in vitro and in vivo experiments
publisher Faculty of Pharmacy, University of Benin
publishDate 2024
url http://umpir.ump.edu.my/id/eprint/42501/1/Glycoproteins%20Isolated%20from%20Tongkat%20Ali%20Plants%20Capable%20t.pdf
http://umpir.ump.edu.my/id/eprint/42501/
https://doi.org/10.26538/tjnpr/v8i6.41
_version_ 1822924651676827648
score 13.244413

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