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Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing

The exposure of the air microbiome in indoor air posed a detrimental health effect to the building occupants compared to the outdoor air. Indoor air in hospitals has been identified as a reservoir for various pathogenic microbes. The conventional culture-dependent method has been widely used to acce...

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Main Authors: Nor Husna, Mat Hussin, Siew, Shing Wei, Mohd Norhafsam, Maghpor, Gan, Han Ming, Hajar Fauzan, Ahmad
Format: Article
Language:English
Published: Elsevier B.V. 2024
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://umpir.ump.edu.my/id/eprint/40536/1/PIIS2215016124000906.pdf
http://umpir.ump.edu.my/id/eprint/40536/
https://doi.org/10.1016/j.mex.2024.102636
https://doi.org/10.1016/j.mex.2024.102636
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spelling my.ump.umpir.405362024-07-17T09:18:24Z http://umpir.ump.edu.my/id/eprint/40536/ Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing Nor Husna, Mat Hussin Siew, Shing Wei Mohd Norhafsam, Maghpor Gan, Han Ming Hajar Fauzan, Ahmad Q Science (General) QR Microbiology The exposure of the air microbiome in indoor air posed a detrimental health effect to the building occupants compared to the outdoor air. Indoor air in hospitals has been identified as a reservoir for various pathogenic microbes. The conventional culture-dependent method has been widely used to access the microbial community in the air. However, it has limited capability in enumerating the complex air microbiome communities, as some of the air microbiomes are uncultivable, slow-growers, and require specific media for cultivation. Here, we utilized a culture-independent method via amplicon sequencing to target the V3 region of 16S rRNA from the pool of total genomic DNA extracted from the dust samples taken from hospital interiors. This method will help occupational health practitioners, researchers, and health authorities to efficiently and comprehensively monitor the presence of harmful air microbiome thus take appropriate action in controlling and minimizing the health risks to the hospital occupants. Key features; • Culture-independent methods offer fast, comprehensive, and unbias profiles of pathogenic and non-pathogenic bacteria from the air microbiomes. • Unlike the culture-dependent method, amplicon sequencing allows bacteria identification to the lowest taxonomy levels. Elsevier B.V. 2024-02-28 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/40536/1/PIIS2215016124000906.pdf Nor Husna, Mat Hussin and Siew, Shing Wei and Mohd Norhafsam, Maghpor and Gan, Han Ming and Hajar Fauzan, Ahmad (2024) Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing. MethodsX, 12 (102636). pp. 1-10. ISSN 2215-0161. (Published) https://doi.org/10.1016/j.mex.2024.102636 https://doi.org/10.1016/j.mex.2024.102636
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Nor Husna, Mat Hussin
Siew, Shing Wei
Mohd Norhafsam, Maghpor
Gan, Han Ming
Hajar Fauzan, Ahmad
Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing
description The exposure of the air microbiome in indoor air posed a detrimental health effect to the building occupants compared to the outdoor air. Indoor air in hospitals has been identified as a reservoir for various pathogenic microbes. The conventional culture-dependent method has been widely used to access the microbial community in the air. However, it has limited capability in enumerating the complex air microbiome communities, as some of the air microbiomes are uncultivable, slow-growers, and require specific media for cultivation. Here, we utilized a culture-independent method via amplicon sequencing to target the V3 region of 16S rRNA from the pool of total genomic DNA extracted from the dust samples taken from hospital interiors. This method will help occupational health practitioners, researchers, and health authorities to efficiently and comprehensively monitor the presence of harmful air microbiome thus take appropriate action in controlling and minimizing the health risks to the hospital occupants. Key features; • Culture-independent methods offer fast, comprehensive, and unbias profiles of pathogenic and non-pathogenic bacteria from the air microbiomes. • Unlike the culture-dependent method, amplicon sequencing allows bacteria identification to the lowest taxonomy levels.
format Article
author Nor Husna, Mat Hussin
Siew, Shing Wei
Mohd Norhafsam, Maghpor
Gan, Han Ming
Hajar Fauzan, Ahmad
author_facet Nor Husna, Mat Hussin
Siew, Shing Wei
Mohd Norhafsam, Maghpor
Gan, Han Ming
Hajar Fauzan, Ahmad
author_sort Nor Husna, Mat Hussin
title Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing
title_short Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing
title_full Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing
title_fullStr Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing
title_full_unstemmed Method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing
title_sort method for detection of pathogenic bacteria from indoor air microbiome samples using high-throughput amplicon sequencing
publisher Elsevier B.V.
publishDate 2024
url http://umpir.ump.edu.my/id/eprint/40536/1/PIIS2215016124000906.pdf
http://umpir.ump.edu.my/id/eprint/40536/
https://doi.org/10.1016/j.mex.2024.102636
https://doi.org/10.1016/j.mex.2024.102636
_version_ 1822924516322443264
score 13.235796

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