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Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia

Decaffeination by microbial degradation is currently the most optimal and low-cost approach, involving only microbial cells and/or their enzymes. The bacterium was characterized using a series of biochemical tests. Positive results were obtained from carbohydrate fermentation, citrate utilization, a...

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Main Authors: Dullah, Elvina Clarie, Mohd Fazli, Farida Asras
Format: Article
Language:English
Published: DRPM Universitas Indonesia 2023
Subjects:
Q Science (General)
S Agriculture (General)
Online Access:http://umpir.ump.edu.my/id/eprint/40400/1/Isolation%20and%20Characterization%20of%20Caffeine-Degrading%20Bacteria.pdf
http://umpir.ump.edu.my/id/eprint/40400/
https://doi.org/10.7454/mst.v27i3.1462
https://doi.org/10.7454/mst.v27i3.1462
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spelling my.ump.umpir.404002024-02-16T07:59:13Z http://umpir.ump.edu.my/id/eprint/40400/ Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia Dullah, Elvina Clarie Mohd Fazli, Farida Asras Q Science (General) S Agriculture (General) Decaffeination by microbial degradation is currently the most optimal and low-cost approach, involving only microbial cells and/or their enzymes. The bacterium was characterized using a series of biochemical tests. Positive results were obtained from carbohydrate fermentation, citrate utilization, and catalase tests, while negative results were obtained from Voges-Proskauer (VP) and indole tests. Three different caffeine concentrations of 0.25%, 0.4%, and 2% were tested and measured through Gas Chromatography-Mass Spectrophotometry (GC-MS) analysis. The highest caffeine reduction (89.25%) was found when 0.25% caffeine was used in the media. Only a small amount of caffeine was reduced to 0.4% and 2%, with 34.78% and 46.16%, respectively. Under microscopic observation, the shape of the isolated bacteria was rod bacilli and was stained with a pink color, which indicates Gram-negative bacteria. Comparing the results with previous research and observing the color of the pure culture, which revealed a yellow color, bacteria isolated from the coffee plantation area can be concluded to be a partially identified Pseudomonas sp. DRPM Universitas Indonesia 2023 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/40400/1/Isolation%20and%20Characterization%20of%20Caffeine-Degrading%20Bacteria.pdf Dullah, Elvina Clarie and Mohd Fazli, Farida Asras (2023) Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia. Makara Journal of Technology, 27 (3). pp. 93-98. ISSN 2355-2786. (Published) https://doi.org/10.7454/mst.v27i3.1462 https://doi.org/10.7454/mst.v27i3.1462
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
S Agriculture (General)
spellingShingle Q Science (General)
S Agriculture (General)
Dullah, Elvina Clarie
Mohd Fazli, Farida Asras
Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia
description Decaffeination by microbial degradation is currently the most optimal and low-cost approach, involving only microbial cells and/or their enzymes. The bacterium was characterized using a series of biochemical tests. Positive results were obtained from carbohydrate fermentation, citrate utilization, and catalase tests, while negative results were obtained from Voges-Proskauer (VP) and indole tests. Three different caffeine concentrations of 0.25%, 0.4%, and 2% were tested and measured through Gas Chromatography-Mass Spectrophotometry (GC-MS) analysis. The highest caffeine reduction (89.25%) was found when 0.25% caffeine was used in the media. Only a small amount of caffeine was reduced to 0.4% and 2%, with 34.78% and 46.16%, respectively. Under microscopic observation, the shape of the isolated bacteria was rod bacilli and was stained with a pink color, which indicates Gram-negative bacteria. Comparing the results with previous research and observing the color of the pure culture, which revealed a yellow color, bacteria isolated from the coffee plantation area can be concluded to be a partially identified Pseudomonas sp.
format Article
author Dullah, Elvina Clarie
Mohd Fazli, Farida Asras
author_facet Dullah, Elvina Clarie
Mohd Fazli, Farida Asras
author_sort Dullah, Elvina Clarie
title Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia
title_short Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia
title_full Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia
title_fullStr Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia
title_full_unstemmed Isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in Malaysia
title_sort isolation and characterization of caffeine-degrading bacteria from coffee plantation areas in malaysia
publisher DRPM Universitas Indonesia
publishDate 2023
url http://umpir.ump.edu.my/id/eprint/40400/1/Isolation%20and%20Characterization%20of%20Caffeine-Degrading%20Bacteria.pdf
http://umpir.ump.edu.my/id/eprint/40400/
https://doi.org/10.7454/mst.v27i3.1462
https://doi.org/10.7454/mst.v27i3.1462
_version_ 1822924149354397696
score 13.244413

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