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Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations

In the current study, a novel high-performance liquid chromatographic method is being developed and validated to estimate benidipine hydrochloride in lipid-based pharmaceutical formulations including transfersome, ethosome, and transethosome. The chromatographic separation was accomplished on a ZORB...

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Main Authors: AL-Japairai, Khater, Syed Mahmood, ., Mohd Azmir, Arifin, Nadiya, Abdul Halim, Almurisi, Samah Hamed
Format: Article
Language:English
Published: Cosmos Scholars Publishing House 2022
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/40283/1/Development%20and%20Validation%20of%20HPLC%20Method%20for%20Determination.pdf
http://umpir.ump.edu.my/id/eprint/40283/
https://doi.org/10.15379/ijmst.v9i2.1047
https://doi.org/10.15379/ijmst.v9i2.1047
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spelling my.ump.umpir.402832024-02-07T03:42:27Z http://umpir.ump.edu.my/id/eprint/40283/ Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations AL-Japairai, Khater Syed Mahmood, . Mohd Azmir, Arifin Nadiya, Abdul Halim Almurisi, Samah Hamed TP Chemical technology In the current study, a novel high-performance liquid chromatographic method is being developed and validated to estimate benidipine hydrochloride in lipid-based pharmaceutical formulations including transfersome, ethosome, and transethosome. The chromatographic separation was accomplished on a ZORBAX Eclipse Plus C18 (4.6mm x 150mm) analytical column with a mobile phase consisting of a mixture of methanol and 50 mM phosphate buffer solution at a ratio of 70:30 (v/v). A standard calibration curve was used to quantitatively determine the medication at a UV wavelength of 237 nm. The limit of detection (LOD) and limit of quantitation (LOQ) were determined to be 0.005µg/ml and 0.015µg/ml, respectively. The relative standard deviation (%RSD) of the intra-day and inter-day studies for benidipine hydrochloride was less than 2%, and the percentage recovery of benidipine hydrochloride was found to be in the range of 98.57-100.27%. The method is specific, linear, accurate, precise, robust, and sensitive for its intended purpose, according to the results of the method validation. To determine the amount of benidipine hydrochloride and the effectiveness of drug entrapment in lipid-based formulated membrane like cell structures namely Transfersomes, Ethosomes, and Transethosomes the current method was successfully applied. Cosmos Scholars Publishing House 2022-10 Article PeerReviewed pdf en cc_by_nc http://umpir.ump.edu.my/id/eprint/40283/1/Development%20and%20Validation%20of%20HPLC%20Method%20for%20Determination.pdf AL-Japairai, Khater and Syed Mahmood, . and Mohd Azmir, Arifin and Nadiya, Abdul Halim and Almurisi, Samah Hamed (2022) Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations. International Journal of Membrane Science and Technology, 9 (2). pp. 40-47. ISSN 2410 -1869. (Published) https://doi.org/10.15379/ijmst.v9i2.1047 https://doi.org/10.15379/ijmst.v9i2.1047
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
AL-Japairai, Khater
Syed Mahmood, .
Mohd Azmir, Arifin
Nadiya, Abdul Halim
Almurisi, Samah Hamed
Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations
description In the current study, a novel high-performance liquid chromatographic method is being developed and validated to estimate benidipine hydrochloride in lipid-based pharmaceutical formulations including transfersome, ethosome, and transethosome. The chromatographic separation was accomplished on a ZORBAX Eclipse Plus C18 (4.6mm x 150mm) analytical column with a mobile phase consisting of a mixture of methanol and 50 mM phosphate buffer solution at a ratio of 70:30 (v/v). A standard calibration curve was used to quantitatively determine the medication at a UV wavelength of 237 nm. The limit of detection (LOD) and limit of quantitation (LOQ) were determined to be 0.005µg/ml and 0.015µg/ml, respectively. The relative standard deviation (%RSD) of the intra-day and inter-day studies for benidipine hydrochloride was less than 2%, and the percentage recovery of benidipine hydrochloride was found to be in the range of 98.57-100.27%. The method is specific, linear, accurate, precise, robust, and sensitive for its intended purpose, according to the results of the method validation. To determine the amount of benidipine hydrochloride and the effectiveness of drug entrapment in lipid-based formulated membrane like cell structures namely Transfersomes, Ethosomes, and Transethosomes the current method was successfully applied.
format Article
author AL-Japairai, Khater
Syed Mahmood, .
Mohd Azmir, Arifin
Nadiya, Abdul Halim
Almurisi, Samah Hamed
author_facet AL-Japairai, Khater
Syed Mahmood, .
Mohd Azmir, Arifin
Nadiya, Abdul Halim
Almurisi, Samah Hamed
author_sort AL-Japairai, Khater
title Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations
title_short Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations
title_full Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations
title_fullStr Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations
title_full_unstemmed Development and validation of HPLC method for determination of benidipine hydrochloride in lipid vesicles formulations
title_sort development and validation of hplc method for determination of benidipine hydrochloride in lipid vesicles formulations
publisher Cosmos Scholars Publishing House
publishDate 2022
url http://umpir.ump.edu.my/id/eprint/40283/1/Development%20and%20Validation%20of%20HPLC%20Method%20for%20Determination.pdf
http://umpir.ump.edu.my/id/eprint/40283/
https://doi.org/10.15379/ijmst.v9i2.1047
https://doi.org/10.15379/ijmst.v9i2.1047
_version_ 1822924137904996352
score 13.235796

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