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The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments

In this study, the Tongkat Ali plants were evaluated for effect on testosterone concentrations and their order in boosting the steroidal hormone from the highest to lowest ranked. Eurycoma longifolia(EL), Stema tuberosa(ST), and Polyathia bullata(PB) are collectively referred to as “Tong...

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Main Authors: Sharifah Aminah, Tuan Said, Fatinah, Ahmad, Chakravarthi, Srikumar, Palliah, Jaya Vejayan
Format: Article
Language:English
Published: Faculty of Pharmacy, University of Benin 2023
Subjects:
HD28 Management. Industrial Management
Q Science (General)
R Medicine (General)
Online Access:http://umpir.ump.edu.my/id/eprint/39511/1/31-TJNPR-2023-M516-Galley-Proof-C-F.pdf
http://umpir.ump.edu.my/id/eprint/39511/
http://www.doi.org/10.26538/tjnpr/v7i11.31
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spelling my.ump.umpir.395112023-12-06T00:35:07Z http://umpir.ump.edu.my/id/eprint/39511/ The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments Sharifah Aminah, Tuan Said Fatinah, Ahmad Chakravarthi, Srikumar Palliah, Jaya Vejayan HD28 Management. Industrial Management Q Science (General) R Medicine (General) In this study, the Tongkat Ali plants were evaluated for effect on testosterone concentrations and their order in boosting the steroidal hormone from the highest to lowest ranked. Eurycoma longifolia(EL), Stema tuberosa(ST), and Polyathia bullata(PB) are collectively referred to as “Tongkat Ali”. The roots were dried and powdered, then extracted with water under reflux. Size-exclusion chromatography was utilized to isolate the protein fraction, which was subsequently characterized using the Bradford Assay and SDS-PAGE. LC-MS was used to test for the presence of natural testosterone within the Tongkat Ali plants. For in vitroand in vivoevaluations, each plant extract was treated with TM-3 Leydig cells (50 μg/mL) for 72 hours and administered in mice (6 mg/mL) twice/day for 20 days. The extraction of EL, ST, and PB yielded 0.74%, 0.46%, and 0.34% w/w of total protein, respectively. SDS-PAGE analysis revealed a single band between 10 and 15 kDa. In vitroevaluations showed that extracts of EL, ST, and PB increased testosterone secretion by 56.02 nmol/L (41.1% compared to the untreated controls), 40.49 nmol/L (18.65%) and 36.99 nmol/L (10.93%), respectively. In the in vivostudies, EL extract showed the highest testosterone concentration at 3.85 nmol/L (51.18% compared to the untreated controls), followed by ST with 3.35 nmol/L (43.95%) and lastly, PB at only 1.88 nmol/L (9.1%). Tongkat Ali plants boosted the male hormones in both in vitroand in vivostudies, with the order being EL>ST>PB. Faculty of Pharmacy, University of Benin 2023-12-01 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/39511/1/31-TJNPR-2023-M516-Galley-Proof-C-F.pdf Sharifah Aminah, Tuan Said and Fatinah, Ahmad and Chakravarthi, Srikumar and Palliah, Jaya Vejayan (2023) The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments. Tropical Journal of Natural Product Research, 7 (11). pp. 5236-5243. ISSN 2616-0684. (Published) http://www.doi.org/10.26538/tjnpr/v7i11.31
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic HD28 Management. Industrial Management
Q Science (General)
R Medicine (General)
spellingShingle HD28 Management. Industrial Management
Q Science (General)
R Medicine (General)
Sharifah Aminah, Tuan Said
Fatinah, Ahmad
Chakravarthi, Srikumar
Palliah, Jaya Vejayan
The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments
description In this study, the Tongkat Ali plants were evaluated for effect on testosterone concentrations and their order in boosting the steroidal hormone from the highest to lowest ranked. Eurycoma longifolia(EL), Stema tuberosa(ST), and Polyathia bullata(PB) are collectively referred to as “Tongkat Ali”. The roots were dried and powdered, then extracted with water under reflux. Size-exclusion chromatography was utilized to isolate the protein fraction, which was subsequently characterized using the Bradford Assay and SDS-PAGE. LC-MS was used to test for the presence of natural testosterone within the Tongkat Ali plants. For in vitroand in vivoevaluations, each plant extract was treated with TM-3 Leydig cells (50 μg/mL) for 72 hours and administered in mice (6 mg/mL) twice/day for 20 days. The extraction of EL, ST, and PB yielded 0.74%, 0.46%, and 0.34% w/w of total protein, respectively. SDS-PAGE analysis revealed a single band between 10 and 15 kDa. In vitroevaluations showed that extracts of EL, ST, and PB increased testosterone secretion by 56.02 nmol/L (41.1% compared to the untreated controls), 40.49 nmol/L (18.65%) and 36.99 nmol/L (10.93%), respectively. In the in vivostudies, EL extract showed the highest testosterone concentration at 3.85 nmol/L (51.18% compared to the untreated controls), followed by ST with 3.35 nmol/L (43.95%) and lastly, PB at only 1.88 nmol/L (9.1%). Tongkat Ali plants boosted the male hormones in both in vitroand in vivostudies, with the order being EL>ST>PB.
format Article
author Sharifah Aminah, Tuan Said
Fatinah, Ahmad
Chakravarthi, Srikumar
Palliah, Jaya Vejayan
author_facet Sharifah Aminah, Tuan Said
Fatinah, Ahmad
Chakravarthi, Srikumar
Palliah, Jaya Vejayan
author_sort Sharifah Aminah, Tuan Said
title The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments
title_short The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments
title_full The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments
title_fullStr The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments
title_full_unstemmed The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments
title_sort ranking of tongkat ali plants to boost testosterone hormone evaluated in both in vitro and in vivo experiments
publisher Faculty of Pharmacy, University of Benin
publishDate 2023
url http://umpir.ump.edu.my/id/eprint/39511/1/31-TJNPR-2023-M516-Galley-Proof-C-F.pdf
http://umpir.ump.edu.my/id/eprint/39511/
http://www.doi.org/10.26538/tjnpr/v7i11.31
_version_ 1822923907113418752
score 13.244413

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