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Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology

Recently, the widespread use of proteases in industries, such as detergent, food, and pharmaceutical sectors, has increased the demand for proteolytic enzymes in the global market. Although the main sources of commercial proteases are animals and microorganisms, the potential of using nonconventiona...

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Main Authors: Nur Fatin Nadia, Mohd Dzakir, Erna Normaya, Abdullah, Nur Amanina, Hassanuddin, Syamimi Sulfiza, Shamsuri, Anwar, Iqbal, Mohd Bijarimi, Mat Piah, Mohammad Norazmi, Ahmad
Format: Conference or Workshop Item
Language:English
English
Published: Malaysian Institute of Chemistry 2023
Subjects:
QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/38988/1/Optimization%20of%20Protease%20Extraction%20from%20Ridged%20Gourd%20%28Luffa%20acutangula%29.pdf
http://umpir.ump.edu.my/id/eprint/38988/2/Optimization%20of%20protease%20extraction%20from%20ridged%20gourd%20%28Luffa%20acutangula%29_ABS.pdf
http://umpir.ump.edu.my/id/eprint/38988/
https://doi.org/10.55373/MJCHEM.V25I3.391
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spelling my.ump.umpir.389882023-11-14T02:57:20Z http://umpir.ump.edu.my/id/eprint/38988/ Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology Nur Fatin Nadia, Mohd Dzakir Erna Normaya, Abdullah Nur Amanina, Hassanuddin Syamimi Sulfiza, Shamsuri Anwar, Iqbal Mohd Bijarimi, Mat Piah Mohammad Norazmi, Ahmad QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Recently, the widespread use of proteases in industries, such as detergent, food, and pharmaceutical sectors, has increased the demand for proteolytic enzymes in the global market. Although the main sources of commercial proteases are animals and microorganisms, the potential of using nonconventional sources, especially plants, should not be overlooked. In this study, proteolytic enzymes were extracted from the sarcocarp of ridged gourd (Luffa acutangula), and the effect of the extraction process on protease activity was evaluated. The crude enzyme was optimized via response surface methodology (RSM) using a central composite rotatable design (CCRD). Four independent variables were studied, namely, the pH, the concentrations of Triton X-100 (TX-100) and 2-mercaptoethanol, and the mixing time. The optimum level of each variable based on the RSM model was determined: at pH 6.38, 4.99% (v/v) TX-100, 0.15 M 2-mercaptoethanol, and a mixing time of 4.09 min, the optimum protease activity was estimated at 1.35 U/g. A verification test revealed satisfactory agreement between the model and experimental results with 96% desirability, indicating that the quadratic model generated from RSM is significant. Malaysian Institute of Chemistry 2023-06-29 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/38988/1/Optimization%20of%20Protease%20Extraction%20from%20Ridged%20Gourd%20%28Luffa%20acutangula%29.pdf pdf en http://umpir.ump.edu.my/id/eprint/38988/2/Optimization%20of%20protease%20extraction%20from%20ridged%20gourd%20%28Luffa%20acutangula%29_ABS.pdf Nur Fatin Nadia, Mohd Dzakir and Erna Normaya, Abdullah and Nur Amanina, Hassanuddin and Syamimi Sulfiza, Shamsuri and Anwar, Iqbal and Mohd Bijarimi, Mat Piah and Mohammad Norazmi, Ahmad (2023) Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology. In: 3rd IKMPB Online Symposium 2023 - Innovation in Chemistry Towards Sustainable Development, 29 June 2023 , Online. pp. 391-399., 25 (3). ISSN 1511-2292 https://doi.org/10.55373/MJCHEM.V25I3.391
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
topic QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
spellingShingle QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
Nur Fatin Nadia, Mohd Dzakir
Erna Normaya, Abdullah
Nur Amanina, Hassanuddin
Syamimi Sulfiza, Shamsuri
Anwar, Iqbal
Mohd Bijarimi, Mat Piah
Mohammad Norazmi, Ahmad
Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology
description Recently, the widespread use of proteases in industries, such as detergent, food, and pharmaceutical sectors, has increased the demand for proteolytic enzymes in the global market. Although the main sources of commercial proteases are animals and microorganisms, the potential of using nonconventional sources, especially plants, should not be overlooked. In this study, proteolytic enzymes were extracted from the sarcocarp of ridged gourd (Luffa acutangula), and the effect of the extraction process on protease activity was evaluated. The crude enzyme was optimized via response surface methodology (RSM) using a central composite rotatable design (CCRD). Four independent variables were studied, namely, the pH, the concentrations of Triton X-100 (TX-100) and 2-mercaptoethanol, and the mixing time. The optimum level of each variable based on the RSM model was determined: at pH 6.38, 4.99% (v/v) TX-100, 0.15 M 2-mercaptoethanol, and a mixing time of 4.09 min, the optimum protease activity was estimated at 1.35 U/g. A verification test revealed satisfactory agreement between the model and experimental results with 96% desirability, indicating that the quadratic model generated from RSM is significant.
format Conference or Workshop Item
author Nur Fatin Nadia, Mohd Dzakir
Erna Normaya, Abdullah
Nur Amanina, Hassanuddin
Syamimi Sulfiza, Shamsuri
Anwar, Iqbal
Mohd Bijarimi, Mat Piah
Mohammad Norazmi, Ahmad
author_facet Nur Fatin Nadia, Mohd Dzakir
Erna Normaya, Abdullah
Nur Amanina, Hassanuddin
Syamimi Sulfiza, Shamsuri
Anwar, Iqbal
Mohd Bijarimi, Mat Piah
Mohammad Norazmi, Ahmad
author_sort Nur Fatin Nadia, Mohd Dzakir
title Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology
title_short Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology
title_full Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology
title_fullStr Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology
title_full_unstemmed Optimization of protease extraction from ridged gourd (Luffa acutangula) sarcocarp via response surface methodology
title_sort optimization of protease extraction from ridged gourd (luffa acutangula) sarcocarp via response surface methodology
publisher Malaysian Institute of Chemistry
publishDate 2023
url http://umpir.ump.edu.my/id/eprint/38988/1/Optimization%20of%20Protease%20Extraction%20from%20Ridged%20Gourd%20%28Luffa%20acutangula%29.pdf
http://umpir.ump.edu.my/id/eprint/38988/2/Optimization%20of%20protease%20extraction%20from%20ridged%20gourd%20%28Luffa%20acutangula%29_ABS.pdf
http://umpir.ump.edu.my/id/eprint/38988/
https://doi.org/10.55373/MJCHEM.V25I3.391
_version_ 1822923804678029312
score 13.235796

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