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Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study

An analytical method based on solid-phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination of five illicit drugs, namely amphetamine (AM), methamphetamine (MA), 3,4- methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), and morphin...

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Main Authors: Mohammad Azimuddin, Izani, Nurul `Azyyati, Sabri, Nurul Nadiah, Hamidon, Kooi, Yeong Khaw, Iekhsan, Othman, Mueller, Jochen, Tay, Joo Hui
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2023
Subjects:
QD Chemistry
TD Environmental technology. Sanitary engineering
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/37910/1/Izani_27_1_1.pdf
http://umpir.ump.edu.my/id/eprint/37910/
https://mjas.analis.com.my/mjas/v27_n1/pdf/Izani_27_1_1.pdf
https://mjas.analis.com.my/mjas/v27_n1/pdf/Izani_27_1_1.pdf
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spelling my.ump.umpir.379102023-07-13T07:14:55Z http://umpir.ump.edu.my/id/eprint/37910/ Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study Mohammad Azimuddin, Izani Nurul `Azyyati, Sabri Nurul Nadiah, Hamidon Kooi, Yeong Khaw Iekhsan, Othman Mueller, Jochen Tay, Joo Hui QD Chemistry TD Environmental technology. Sanitary engineering TP Chemical technology An analytical method based on solid-phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination of five illicit drugs, namely amphetamine (AM), methamphetamine (MA), 3,4- methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), and morphine (MOR) in municipal wastewater has been optimized and validated. Sample preparation was performed using Oasis MCX SPE cartridges. LC separation was performed using a Zorbax Eclipse Plus C18 RRHD column. The linearity of the calibration curve was between 5 ng/mL and 250 ng/mL, with determination coefficient (R2 ) greater than 0.99, except for morphine. The mean recoveries of target analytes ranged from 91.6 to 112%, and the method demonstrated good inter-day repeatability (coefficient of variation, CV ranged from 2 to 19%). The limit of detection (LOD) for AM, MA, MDMA, MDA, and MOR was 0.29, 0.37, 0.86, 1.09 and 7.56 ng/mL, respectively. The method was applied to municipal wastewater samples collected from sewage treatment plants in Kuantan, Pahang, in which AM, MA and MDA were detected in all 3 samples. Universiti Kebangsaan Malaysia 2023-02-22 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/37910/1/Izani_27_1_1.pdf Mohammad Azimuddin, Izani and Nurul `Azyyati, Sabri and Nurul Nadiah, Hamidon and Kooi, Yeong Khaw and Iekhsan, Othman and Mueller, Jochen and Tay, Joo Hui (2023) Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study. Malaysian Journal of Analytical Sciences, 27 (1). pp. 1-7. ISSN 1394-2506. (Published) https://mjas.analis.com.my/mjas/v27_n1/pdf/Izani_27_1_1.pdf https://mjas.analis.com.my/mjas/v27_n1/pdf/Izani_27_1_1.pdf
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
TD Environmental technology. Sanitary engineering
TP Chemical technology
spellingShingle QD Chemistry
TD Environmental technology. Sanitary engineering
TP Chemical technology
Mohammad Azimuddin, Izani
Nurul `Azyyati, Sabri
Nurul Nadiah, Hamidon
Kooi, Yeong Khaw
Iekhsan, Othman
Mueller, Jochen
Tay, Joo Hui
Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study
description An analytical method based on solid-phase extraction (SPE) liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination of five illicit drugs, namely amphetamine (AM), methamphetamine (MA), 3,4- methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), and morphine (MOR) in municipal wastewater has been optimized and validated. Sample preparation was performed using Oasis MCX SPE cartridges. LC separation was performed using a Zorbax Eclipse Plus C18 RRHD column. The linearity of the calibration curve was between 5 ng/mL and 250 ng/mL, with determination coefficient (R2 ) greater than 0.99, except for morphine. The mean recoveries of target analytes ranged from 91.6 to 112%, and the method demonstrated good inter-day repeatability (coefficient of variation, CV ranged from 2 to 19%). The limit of detection (LOD) for AM, MA, MDMA, MDA, and MOR was 0.29, 0.37, 0.86, 1.09 and 7.56 ng/mL, respectively. The method was applied to municipal wastewater samples collected from sewage treatment plants in Kuantan, Pahang, in which AM, MA and MDA were detected in all 3 samples.
format Article
author Mohammad Azimuddin, Izani
Nurul `Azyyati, Sabri
Nurul Nadiah, Hamidon
Kooi, Yeong Khaw
Iekhsan, Othman
Mueller, Jochen
Tay, Joo Hui
author_facet Mohammad Azimuddin, Izani
Nurul `Azyyati, Sabri
Nurul Nadiah, Hamidon
Kooi, Yeong Khaw
Iekhsan, Othman
Mueller, Jochen
Tay, Joo Hui
author_sort Mohammad Azimuddin, Izani
title Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study
title_short Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study
title_full Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study
title_fullStr Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study
title_full_unstemmed Analysis of illicit drugs in municipal wastewater using LCMS/MS: A method validation study
title_sort analysis of illicit drugs in municipal wastewater using lcms/ms: a method validation study
publisher Universiti Kebangsaan Malaysia
publishDate 2023
url http://umpir.ump.edu.my/id/eprint/37910/1/Izani_27_1_1.pdf
http://umpir.ump.edu.my/id/eprint/37910/
https://mjas.analis.com.my/mjas/v27_n1/pdf/Izani_27_1_1.pdf
https://mjas.analis.com.my/mjas/v27_n1/pdf/Izani_27_1_1.pdf
_version_ 1772811341702103040
score 13.211869

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