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Pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in Malaysia

Background: Colorectal cancer (CRC) is the second most lethal disease with about 1.9 million new cases and 0.9 million fatalities worldwide in 2020. It is expected that the CRC prevalence to rise steadily each year. Several studies have linked the gut microbiome to CRC, particularly emphasizing the...

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Main Authors: Aisyah, Yunus, Norfilza, Mohd Mokhtar, Raja Affendi, Raja Ali, Siti Maryam, Ahmad Kendong, Dennis, Sandris Nielsen, Hajar Fauzan, Ahmad
Format: Conference or Workshop Item
Language:English
English
Published: BMJ Publishing Group Ltd & British Society of Gastroenterology 2023
Subjects:
Q Science (General)
R Medicine (General)
Online Access:http://umpir.ump.edu.my/id/eprint/37909/1/IDDF%20-%20e-Poster%20Pathogenic%20fungi%20of%20rhodotorula%20dairenensis%20is%20linked%20with%20colorectal%20cancer%20patients%20in%20malaysia.pdf
http://umpir.ump.edu.my/id/eprint/37909/2/Pathogenic%20fungi%20of%20rhodotorula%20dairenensis.pdf
http://umpir.ump.edu.my/id/eprint/37909/
http://dx.doi.org/10.1136/gutjnl-2023-IDDF.116
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spelling my.ump.umpir.379092023-07-12T07:57:48Z http://umpir.ump.edu.my/id/eprint/37909/ Pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in Malaysia Aisyah, Yunus Norfilza, Mohd Mokhtar Raja Affendi, Raja Ali Siti Maryam, Ahmad Kendong Dennis, Sandris Nielsen Hajar Fauzan, Ahmad Q Science (General) R Medicine (General) Background: Colorectal cancer (CRC) is the second most lethal disease with about 1.9 million new cases and 0.9 million fatalities worldwide in 2020. It is expected that the CRC prevalence to rise steadily each year. Several studies have linked the gut microbiome to CRC, particularly emphasizing the prokaryotic communities’ functions. However, it is unclear how other gut microbiota components, such as fungal communities, could be related to the pathogenesis of CRC. Hence, we aimed to explore the role of opportunistic fungal pathogens and the host’s phenotypes among CRC patients. Methods: Biopsy samples were obtained during colonoscopy sessions from 64 individuals. Of which, 32 are colorectal cancer patients comprising the early-onset CRC, and late-onset CRC groups, 22 are diagnosed with polyps during colonoscopy and the remaining are rectal swabs from normal individuals without any previous disease history. Informed consents were obtained from all patients before collecting their biopsy samples. The g DNA were extracted using Ultra Deep Microbiome Prep Kit. Prior to sequencing, the amplicons of microbial genome libraries were by targeting the ITS1 regions. Finally, the microbial genomic data were analysed using state-of-art bioinformatic tools. Results: A total of 6,477,706 read counts were generated, representing 1,364 amplicon sequence variants of fungi. At phyla, Ascomycota, Basidiomycota, Mortierellomycota, and Chytridiomycota were mainly found in both early and late-onset CRC patients. Moreover, the alpha-diversity showed significant differences between early and late-onset CRC patients, polyps, and normal individuals; Chao1 diversity (p-value = 0.0017509). Based on Linear discriminant analysis Effect Size analysis, the species Rhodotorula dairenensis was found to have a positive correlation for both early and late-onset colorectal cancer patients. Conclusions: Our findings imply the correlation between the presence of opportunistic fungal species Rhodotorula among CRC patients in Malaysia. Previous studies reported that cancer patients are at higher risk for Rhodotorula infection. However, further study is needed in order to elucidate the role of the opportunistic pathogen during disease progression. BMJ Publishing Group Ltd & British Society of Gastroenterology 2023 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/37909/1/IDDF%20-%20e-Poster%20Pathogenic%20fungi%20of%20rhodotorula%20dairenensis%20is%20linked%20with%20colorectal%20cancer%20patients%20in%20malaysia.pdf pdf en http://umpir.ump.edu.my/id/eprint/37909/2/Pathogenic%20fungi%20of%20rhodotorula%20dairenensis.pdf Aisyah, Yunus and Norfilza, Mohd Mokhtar and Raja Affendi, Raja Ali and Siti Maryam, Ahmad Kendong and Dennis, Sandris Nielsen and Hajar Fauzan, Ahmad (2023) Pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in Malaysia. In: International Digestive Disease Forum (IDDF) 2023, 10-11 June 2023 , Hong Kong (Virtual Online). , 72 (Suppl 1). ISSN 0017-5749 http://dx.doi.org/10.1136/gutjnl-2023-IDDF.116
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
topic Q Science (General)
R Medicine (General)
spellingShingle Q Science (General)
R Medicine (General)
Aisyah, Yunus
Norfilza, Mohd Mokhtar
Raja Affendi, Raja Ali
Siti Maryam, Ahmad Kendong
Dennis, Sandris Nielsen
Hajar Fauzan, Ahmad
Pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in Malaysia
description Background: Colorectal cancer (CRC) is the second most lethal disease with about 1.9 million new cases and 0.9 million fatalities worldwide in 2020. It is expected that the CRC prevalence to rise steadily each year. Several studies have linked the gut microbiome to CRC, particularly emphasizing the prokaryotic communities’ functions. However, it is unclear how other gut microbiota components, such as fungal communities, could be related to the pathogenesis of CRC. Hence, we aimed to explore the role of opportunistic fungal pathogens and the host’s phenotypes among CRC patients. Methods: Biopsy samples were obtained during colonoscopy sessions from 64 individuals. Of which, 32 are colorectal cancer patients comprising the early-onset CRC, and late-onset CRC groups, 22 are diagnosed with polyps during colonoscopy and the remaining are rectal swabs from normal individuals without any previous disease history. Informed consents were obtained from all patients before collecting their biopsy samples. The g DNA were extracted using Ultra Deep Microbiome Prep Kit. Prior to sequencing, the amplicons of microbial genome libraries were by targeting the ITS1 regions. Finally, the microbial genomic data were analysed using state-of-art bioinformatic tools. Results: A total of 6,477,706 read counts were generated, representing 1,364 amplicon sequence variants of fungi. At phyla, Ascomycota, Basidiomycota, Mortierellomycota, and Chytridiomycota were mainly found in both early and late-onset CRC patients. Moreover, the alpha-diversity showed significant differences between early and late-onset CRC patients, polyps, and normal individuals; Chao1 diversity (p-value = 0.0017509). Based on Linear discriminant analysis Effect Size analysis, the species Rhodotorula dairenensis was found to have a positive correlation for both early and late-onset colorectal cancer patients. Conclusions: Our findings imply the correlation between the presence of opportunistic fungal species Rhodotorula among CRC patients in Malaysia. Previous studies reported that cancer patients are at higher risk for Rhodotorula infection. However, further study is needed in order to elucidate the role of the opportunistic pathogen during disease progression.
format Conference or Workshop Item
author Aisyah, Yunus
Norfilza, Mohd Mokhtar
Raja Affendi, Raja Ali
Siti Maryam, Ahmad Kendong
Dennis, Sandris Nielsen
Hajar Fauzan, Ahmad
author_facet Aisyah, Yunus
Norfilza, Mohd Mokhtar
Raja Affendi, Raja Ali
Siti Maryam, Ahmad Kendong
Dennis, Sandris Nielsen
Hajar Fauzan, Ahmad
author_sort Aisyah, Yunus
title Pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in Malaysia
title_short Pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in Malaysia
title_full Pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in Malaysia
title_fullStr Pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in Malaysia
title_full_unstemmed Pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in Malaysia
title_sort pathogenic fungi of rhodotorula dairenensis is linked with colorectal cancer patients in malaysia
publisher BMJ Publishing Group Ltd & British Society of Gastroenterology
publishDate 2023
url http://umpir.ump.edu.my/id/eprint/37909/1/IDDF%20-%20e-Poster%20Pathogenic%20fungi%20of%20rhodotorula%20dairenensis%20is%20linked%20with%20colorectal%20cancer%20patients%20in%20malaysia.pdf
http://umpir.ump.edu.my/id/eprint/37909/2/Pathogenic%20fungi%20of%20rhodotorula%20dairenensis.pdf
http://umpir.ump.edu.my/id/eprint/37909/
http://dx.doi.org/10.1136/gutjnl-2023-IDDF.116
_version_ 1772811341577322496
score 13.211869

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