Cover Image

Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)

Factors influencing poly(3-hydroxybutyrate) P(3HB) production by  Cupriavidus necator CCUG52238T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigate...

Full description

Saved in:
Bibliographic Details
Main Authors: Mior Ahmad Khushairi, Mohd Zahari, Jailani, Salihon
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2012
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/3784/1/2012_Mohd_Zahari_et_al_Factors_Affecting.pdf
http://umpir.ump.edu.my/id/eprint/3784/
http://www.hindawi.com/journals/bmri/2012/125865/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ump.umpir.3784
record_format eprints
spelling my.ump.umpir.37842018-02-19T06:43:05Z http://umpir.ump.edu.my/id/eprint/3784/ Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T) Mior Ahmad Khushairi, Mohd Zahari Jailani, Salihon TP Chemical technology Factors influencing poly(3-hydroxybutyrate) P(3HB) production by  Cupriavidus necator CCUG52238T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ± 0.13 g/L and 45 ± 1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3784/1/2012_Mohd_Zahari_et_al_Factors_Affecting.pdf Mior Ahmad Khushairi, Mohd Zahari and Jailani, Salihon (2012) Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T). Journal of Biomedicine and Biotechnology, 2012. http://www.hindawi.com/journals/bmri/2012/125865/
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mior Ahmad Khushairi, Mohd Zahari
Jailani, Salihon
Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
description Factors influencing poly(3-hydroxybutyrate) P(3HB) production by  Cupriavidus necator CCUG52238T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ± 0.13 g/L and 45 ± 1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate.
format Article
author Mior Ahmad Khushairi, Mohd Zahari
Jailani, Salihon
author_facet Mior Ahmad Khushairi, Mohd Zahari
Jailani, Salihon
author_sort Mior Ahmad Khushairi, Mohd Zahari
title Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_short Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_full Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_fullStr Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_full_unstemmed Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)
title_sort factors affecting poly(3-hydroxybutyrate) product ion from oil palm frond juice by cupriavidus necator (ccug52238t)
publisher Hindawi Publishing Corporation
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/3784/1/2012_Mohd_Zahari_et_al_Factors_Affecting.pdf
http://umpir.ump.edu.my/id/eprint/3784/
http://www.hindawi.com/journals/bmri/2012/125865/
_version_ 1643664880509648896
score 13.211869

Similar Items