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Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold

3D bone scaffold is the temporary constructs substrates that enable and activate the bonding and reproduction of osteoinductive cells that lead to the creation of new usable tissues. The objectives of this research study are to synthesize and fabricate bromelain/poly (vinyl alcohol) (PVA) porous sub...

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Main Authors:	Azri, Qurratul Aini, Mat Jahari, Fatini Illyana, Jahir Hussain, Fathima Shahitha, Zulkifli, Farah Hanani
Format:	Article
Language:	English
Published:	Penerbit Universiti Malaysia Pahang 2022
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/37099/1/Synthesis%20and%20Characterization%20of%20Bromelain%20Enzyme.pdf http://umpir.ump.edu.my/id/eprint/37099/ https://doi.org/10.15282/cst.v2i1.8862 https://doi.org/10.15282/cst.v2i1.8862
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spelling	my.ump.umpir.370992023-02-23T06:48:42Z http://umpir.ump.edu.my/id/eprint/37099/ Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold Azri, Qurratul Aini Mat Jahari, Fatini Illyana Jahir Hussain, Fathima Shahitha Zulkifli, Farah Hanani TP Chemical technology 3D bone scaffold is the temporary constructs substrates that enable and activate the bonding and reproduction of osteoinductive cells that lead to the creation of new usable tissues. The objectives of this research study are to synthesize and fabricate bromelain/poly (vinyl alcohol) (PVA) porous substrates by using a lyophilization technique and study the properties of the scaffold subsequently. In this study, bromelain (1, 2 and 3 wt.%) and PVA (15 wt.%) solutions were prepared, then mixed overnight to get homogeneous solution. The solutions were frozen for 24 hours before being freeze-dried under vacuum for 7 days at -80 °C. Thereafter, the porous substrates were crosslinked by using glutaraldehyde and characterized using X-ray Diffraction (XRD), Differential Scanning Calorimetry (DSC) and Fourier Transform Infrared Spectroscopy (FTIR). According to the XRD findings, PVA/bromelain scaffolds exhibits great degree of crystallinity at 21.11° and 41.05°. The FTIR results shows no significant effect on the absorption peak at varied concentration of bromelain in bromelain/PVA substrates. For the DSC result, the glass transition temperature was found in the range of 65 to 75 °C. For TGA curve, it was obvious that the temperatures of the weight loss regions gradually increased with the bromelain content, indicating that bromelain improves the stability of the bromelain/PVA membranes at high temperatures. Bromelain/PVA showed high porosity (>75%), and water-swelling ratio within 50 to 150 percent after 24, 48, and 72 hours immersion period. This newly developed scaffold composition may serve as a foundation for the growth of bone tissue. Penerbit Universiti Malaysia Pahang 2022-12 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/37099/1/Synthesis%20and%20Characterization%20of%20Bromelain%20Enzyme.pdf Azri, Qurratul Aini and Mat Jahari, Fatini Illyana and Jahir Hussain, Fathima Shahitha and Zulkifli, Farah Hanani (2022) Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold. Current Science and Technology (CST), 2 (1). pp. 67-73. ISSN 2785-8804 https://doi.org/10.15282/cst.v2i1.8862 https://doi.org/10.15282/cst.v2i1.8862
institution	Universiti Malaysia Pahang
building	UMP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Pahang
content_source	UMP Institutional Repository
url_provider	http://umpir.ump.edu.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Azri, Qurratul Aini Mat Jahari, Fatini Illyana Jahir Hussain, Fathima Shahitha Zulkifli, Farah Hanani Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold
description	3D bone scaffold is the temporary constructs substrates that enable and activate the bonding and reproduction of osteoinductive cells that lead to the creation of new usable tissues. The objectives of this research study are to synthesize and fabricate bromelain/poly (vinyl alcohol) (PVA) porous substrates by using a lyophilization technique and study the properties of the scaffold subsequently. In this study, bromelain (1, 2 and 3 wt.%) and PVA (15 wt.%) solutions were prepared, then mixed overnight to get homogeneous solution. The solutions were frozen for 24 hours before being freeze-dried under vacuum for 7 days at -80 °C. Thereafter, the porous substrates were crosslinked by using glutaraldehyde and characterized using X-ray Diffraction (XRD), Differential Scanning Calorimetry (DSC) and Fourier Transform Infrared Spectroscopy (FTIR). According to the XRD findings, PVA/bromelain scaffolds exhibits great degree of crystallinity at 21.11° and 41.05°. The FTIR results shows no significant effect on the absorption peak at varied concentration of bromelain in bromelain/PVA substrates. For the DSC result, the glass transition temperature was found in the range of 65 to 75 °C. For TGA curve, it was obvious that the temperatures of the weight loss regions gradually increased with the bromelain content, indicating that bromelain improves the stability of the bromelain/PVA membranes at high temperatures. Bromelain/PVA showed high porosity (>75%), and water-swelling ratio within 50 to 150 percent after 24, 48, and 72 hours immersion period. This newly developed scaffold composition may serve as a foundation for the growth of bone tissue.
format	Article
author	Azri, Qurratul Aini Mat Jahari, Fatini Illyana Jahir Hussain, Fathima Shahitha Zulkifli, Farah Hanani
author_facet	Azri, Qurratul Aini Mat Jahari, Fatini Illyana Jahir Hussain, Fathima Shahitha Zulkifli, Farah Hanani
author_sort	Azri, Qurratul Aini
title	Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold
title_short	Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold
title_full	Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold
title_fullStr	Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold
title_full_unstemmed	Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold
title_sort	synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold
publisher	Penerbit Universiti Malaysia Pahang
publishDate	2022
url	http://umpir.ump.edu.my/id/eprint/37099/1/Synthesis%20and%20Characterization%20of%20Bromelain%20Enzyme.pdf http://umpir.ump.edu.my/id/eprint/37099/ https://doi.org/10.15282/cst.v2i1.8862 https://doi.org/10.15282/cst.v2i1.8862
_version_	1758950526314086400
score	13.211869

Synthesis and characterization of bromelain enzyme/poly (vinyl alcohol) as potential bone scaffold

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