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Isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection

Proteus mirabilis is a clinical pathogen harbouring antibiotic resistance genes and has been identified to cause various healthcare-acquired infections in immunocompromised patients. This study aimed to isolate pathogenic bacteria Proteus mirabilis from microwave pre-treated medical waste. A 100 μL...

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Main Authors: Siew, Shing Wei, Liew, Suet Lai, Mohd Nazli, Kamarulzaman, Hajar Fauzan, Ahmad
Format: Conference or Workshop Item
Language:English
Published: 2022
Subjects:
HD28 Management. Industrial Management
Q Science (General)
T Technology (General)
Online Access:http://umpir.ump.edu.my/id/eprint/36790/1/Isolation%20of%20proteus%20mirabilis%20from%20microwave%20treatment%20of%20medical%20waste%20associated%20with%20urinary%20infection.pdf
http://umpir.ump.edu.my/id/eprint/36790/
https://ncon-pgr.ump.edu.my/index.php/en/?option=com_fileman&view=file&routed=1&name=E-BOOK%20NCON%202022%20.pdf&folder=E-BOOK%20NCON%202022&container=fileman-files
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spelling my.ump.umpir.367902023-02-20T07:18:59Z http://umpir.ump.edu.my/id/eprint/36790/ Isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection Siew, Shing Wei Liew, Suet Lai Mohd Nazli, Kamarulzaman Hajar Fauzan, Ahmad HD28 Management. Industrial Management Q Science (General) T Technology (General) Proteus mirabilis is a clinical pathogen harbouring antibiotic resistance genes and has been identified to cause various healthcare-acquired infections in immunocompromised patients. This study aimed to isolate pathogenic bacteria Proteus mirabilis from microwave pre-treated medical waste. A 100 μL of serially diluted medical waste sample was cultured on Luria Bertani (LB) agar containing ampicillin, and the presumed colonies of P. mirabilis were extracted using FastDNA Stool Mini Kit, followed by species identification via Sanger sequencing. Antibiotic resistant bacteria were successfully cultured, and the swarming behavior was observed on the surface of culture medium. The sequence reads generated from Sanger sequencing compared against reference sequences in NCBI database confirmed that the isolates was P. mirabilis with 99% percentage identity. The presence of antibiotic-resistant P. mirabilis isolated from microwave pretreated medical waste indicated the microwave had minimal effect in eliminating antibiotic resistant bacteria in medical waste. This study also highlights the potential risk of antimicrobial resistance transmission when improperly handled medical wastes are discharged into the environment. Thus, the discovery of pathogenic P. mirabilis from medical waste necessitated public attention to improve medical waste management. 2022-11-15 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/36790/1/Isolation%20of%20proteus%20mirabilis%20from%20microwave%20treatment%20of%20medical%20waste%20associated%20with%20urinary%20infection.pdf Siew, Shing Wei and Liew, Suet Lai and Mohd Nazli, Kamarulzaman and Hajar Fauzan, Ahmad (2022) Isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection. In: The 6th National Conference for Postgraduate Research (NCON-PGR 2022), 15 November 2022 , Virtual Conference, Universiti Malaysia Pahang, Malaysia. p. 71.. https://ncon-pgr.ump.edu.my/index.php/en/?option=com_fileman&view=file&routed=1&name=E-BOOK%20NCON%202022%20.pdf&folder=E-BOOK%20NCON%202022&container=fileman-files
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic HD28 Management. Industrial Management
Q Science (General)
T Technology (General)
spellingShingle HD28 Management. Industrial Management
Q Science (General)
T Technology (General)
Siew, Shing Wei
Liew, Suet Lai
Mohd Nazli, Kamarulzaman
Hajar Fauzan, Ahmad
Isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection
description Proteus mirabilis is a clinical pathogen harbouring antibiotic resistance genes and has been identified to cause various healthcare-acquired infections in immunocompromised patients. This study aimed to isolate pathogenic bacteria Proteus mirabilis from microwave pre-treated medical waste. A 100 μL of serially diluted medical waste sample was cultured on Luria Bertani (LB) agar containing ampicillin, and the presumed colonies of P. mirabilis were extracted using FastDNA Stool Mini Kit, followed by species identification via Sanger sequencing. Antibiotic resistant bacteria were successfully cultured, and the swarming behavior was observed on the surface of culture medium. The sequence reads generated from Sanger sequencing compared against reference sequences in NCBI database confirmed that the isolates was P. mirabilis with 99% percentage identity. The presence of antibiotic-resistant P. mirabilis isolated from microwave pretreated medical waste indicated the microwave had minimal effect in eliminating antibiotic resistant bacteria in medical waste. This study also highlights the potential risk of antimicrobial resistance transmission when improperly handled medical wastes are discharged into the environment. Thus, the discovery of pathogenic P. mirabilis from medical waste necessitated public attention to improve medical waste management.
format Conference or Workshop Item
author Siew, Shing Wei
Liew, Suet Lai
Mohd Nazli, Kamarulzaman
Hajar Fauzan, Ahmad
author_facet Siew, Shing Wei
Liew, Suet Lai
Mohd Nazli, Kamarulzaman
Hajar Fauzan, Ahmad
author_sort Siew, Shing Wei
title Isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection
title_short Isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection
title_full Isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection
title_fullStr Isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection
title_full_unstemmed Isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection
title_sort isolation of proteus mirabilis from microwave treatment of medical waste associated with urinary infection
publishDate 2022
url http://umpir.ump.edu.my/id/eprint/36790/1/Isolation%20of%20proteus%20mirabilis%20from%20microwave%20treatment%20of%20medical%20waste%20associated%20with%20urinary%20infection.pdf
http://umpir.ump.edu.my/id/eprint/36790/
https://ncon-pgr.ump.edu.my/index.php/en/?option=com_fileman&view=file&routed=1&name=E-BOOK%20NCON%202022%20.pdf&folder=E-BOOK%20NCON%202022&container=fileman-files
_version_ 1758578252523241472
score 13.211869

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