Cover Image

Kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor

Sonobioreactor in this study is an integration system which includes the use of a sonicator with a bioreactor by the assembly of a peristaltic pump. The sonobioreactor was being applied to the production of a heterologous protein, lipase by the expression of a recombinant E. coli. The first objectiv...

Full description

Saved in:
Bibliographic Details
Main Author: Ajit, Azilah
Format: Research Book Profile
Language:English
Published: 2016
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/36250/1/Kinetic%20correlations%20of%20gas-liquid%20mass%20transfer%20coefficient%20and%20oxygen%20uptake%20rate%20of%20heterologous%20protein%20cultivation%20by%20sonobioreactor.pdf
http://umpir.ump.edu.my/id/eprint/36250/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ump.umpir.36250
record_format eprints
spelling my.ump.umpir.362502023-01-04T02:11:15Z http://umpir.ump.edu.my/id/eprint/36250/ Kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor Ajit, Azilah TP Chemical technology Sonobioreactor in this study is an integration system which includes the use of a sonicator with a bioreactor by the assembly of a peristaltic pump. The sonobioreactor was being applied to the production of a heterologous protein, lipase by the expression of a recombinant E. coli. The first objective of this study was to transform all the recombinant E. coli into BL21 host. The BL21 was found successful in carrying pTrcHis and pUC8, both carrying lipase gene from Staphylococcus hyicus and Rhizopus oryzae, respectively. Second objective is to investigate the characteristics of 2-L sonobioreactor for the use of recombinant E. coli cultivation. The sonobioreactor has shown some improvement of gas-liquid transfer (kLa} compared to the standalone system. Third objective is to determine the expression of lipase and plasmid stability of recombinant E. coli under sonicated and non-sonicated ultrasound regimens. The non-sonicated cultivation has shown higher lipase activity compared to the sonicated cultivation. The gradual sonication duty cycle has shown images of deformed cells of recombinant E. coli at 9K total magnification via SEM. Performances and kinetics of bioprocesses outcomes in this study may contribute to the current modelling of E. coli fermentations, especially for the recombinant protein production. 2016 Research Book Profile NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/36250/1/Kinetic%20correlations%20of%20gas-liquid%20mass%20transfer%20coefficient%20and%20oxygen%20uptake%20rate%20of%20heterologous%20protein%20cultivation%20by%20sonobioreactor.pdf Ajit, Azilah (2016) Kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor. (Unpublished)
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Ajit, Azilah
Kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor
description Sonobioreactor in this study is an integration system which includes the use of a sonicator with a bioreactor by the assembly of a peristaltic pump. The sonobioreactor was being applied to the production of a heterologous protein, lipase by the expression of a recombinant E. coli. The first objective of this study was to transform all the recombinant E. coli into BL21 host. The BL21 was found successful in carrying pTrcHis and pUC8, both carrying lipase gene from Staphylococcus hyicus and Rhizopus oryzae, respectively. Second objective is to investigate the characteristics of 2-L sonobioreactor for the use of recombinant E. coli cultivation. The sonobioreactor has shown some improvement of gas-liquid transfer (kLa} compared to the standalone system. Third objective is to determine the expression of lipase and plasmid stability of recombinant E. coli under sonicated and non-sonicated ultrasound regimens. The non-sonicated cultivation has shown higher lipase activity compared to the sonicated cultivation. The gradual sonication duty cycle has shown images of deformed cells of recombinant E. coli at 9K total magnification via SEM. Performances and kinetics of bioprocesses outcomes in this study may contribute to the current modelling of E. coli fermentations, especially for the recombinant protein production.
format Research Book Profile
author Ajit, Azilah
author_facet Ajit, Azilah
author_sort Ajit, Azilah
title Kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor
title_short Kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor
title_full Kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor
title_fullStr Kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor
title_full_unstemmed Kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor
title_sort kinetic correlations of gas-liquid mass transfer coefficient and oxygen uptake rate of heterologous protein cultivation by sonobioreactor
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/36250/1/Kinetic%20correlations%20of%20gas-liquid%20mass%20transfer%20coefficient%20and%20oxygen%20uptake%20rate%20of%20heterologous%20protein%20cultivation%20by%20sonobioreactor.pdf
http://umpir.ump.edu.my/id/eprint/36250/
_version_ 1754529724241018880
score 13.211869

Similar Items