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Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products

Clinacanthus nutans has attracted Malaysian public interest due to its high medicinal values in the prevention of cancer. The extraction was done by MeOH at room temperature while chromatography was done on a silica gel RP-18. Six fractions collected from column chromatography were evaluated by the...

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Main Authors: Hazrulrizawati, Abd Hamid, Mashitah, Mohd Yusoff
Format: Research Book Profile
Language:English
Published: 2016
Subjects:
Q Science (General)
T Technology (General)
Online Access:http://umpir.ump.edu.my/id/eprint/36247/1/Development%20of%20chemical%20markers%20and%20chromatographic%20fingerprint%20of%20Clinachantus%20nutans%20for%20evaluation%20of%20therapeutic%20products.pdf
http://umpir.ump.edu.my/id/eprint/36247/
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spelling my.ump.umpir.362472023-01-04T02:04:59Z http://umpir.ump.edu.my/id/eprint/36247/ Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products Hazrulrizawati, Abd Hamid Mashitah, Mohd Yusoff Q Science (General) T Technology (General) Clinacanthus nutans has attracted Malaysian public interest due to its high medicinal values in the prevention of cancer. The extraction was done by MeOH at room temperature while chromatography was done on a silica gel RP-18. Six fractions collected from column chromatography were evaluated by the MTT assay against two breast cancer cell lines: MDA-MB-231 and MCF-7. Amongst the fractions, A12 and A17 were shown to exhibit the most activity, and the compounds were mostly isolated from these fractions. Two sulphur-containing compounds, entadamide C and clinamide D were isolated. Molecular docking simulation studies revealed that entadamide C and clinamide D could bind favourably to the caspase-3 binding site with the binding energy of -4.28 kcal/mol and -4.84 kcal/mol, respectively. 2016 Research Book Profile NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/36247/1/Development%20of%20chemical%20markers%20and%20chromatographic%20fingerprint%20of%20Clinachantus%20nutans%20for%20evaluation%20of%20therapeutic%20products.pdf Hazrulrizawati, Abd Hamid and Mashitah, Mohd Yusoff (2016) Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products. , [Research Book Profile: Research Report] (Unpublished)
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
T Technology (General)
spellingShingle Q Science (General)
T Technology (General)
Hazrulrizawati, Abd Hamid
Mashitah, Mohd Yusoff
Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products
description Clinacanthus nutans has attracted Malaysian public interest due to its high medicinal values in the prevention of cancer. The extraction was done by MeOH at room temperature while chromatography was done on a silica gel RP-18. Six fractions collected from column chromatography were evaluated by the MTT assay against two breast cancer cell lines: MDA-MB-231 and MCF-7. Amongst the fractions, A12 and A17 were shown to exhibit the most activity, and the compounds were mostly isolated from these fractions. Two sulphur-containing compounds, entadamide C and clinamide D were isolated. Molecular docking simulation studies revealed that entadamide C and clinamide D could bind favourably to the caspase-3 binding site with the binding energy of -4.28 kcal/mol and -4.84 kcal/mol, respectively.
format Research Book Profile
author Hazrulrizawati, Abd Hamid
Mashitah, Mohd Yusoff
author_facet Hazrulrizawati, Abd Hamid
Mashitah, Mohd Yusoff
author_sort Hazrulrizawati, Abd Hamid
title Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products
title_short Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products
title_full Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products
title_fullStr Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products
title_full_unstemmed Development of chemical markers and chromatographic fingerprint of Clinachantus nutans for evaluation of therapeutic products
title_sort development of chemical markers and chromatographic fingerprint of clinachantus nutans for evaluation of therapeutic products
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/36247/1/Development%20of%20chemical%20markers%20and%20chromatographic%20fingerprint%20of%20Clinachantus%20nutans%20for%20evaluation%20of%20therapeutic%20products.pdf
http://umpir.ump.edu.my/id/eprint/36247/
_version_ 1754529723841511424
score 13.211869

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