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A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis

A comprehensive research has been carried out on L-glutaminase producers from marine bacteria. Among the evaluated isolates, Kosakonia radicincitans was found to be a L-glutaminase producer from a marine environment, having the highest enzymatic activities. The optimal condition for L-glutaminase pr...

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Main Authors: Aizi Nor Mazila, Ramli, Nur Dini, Johari, Mohd Akmal, Azhar, Rohaida, Che Man, Hazrulrizawati, Hamid
Format: Article
Language:English
Published: Springer International Publishing 2021
Subjects:
Q Science (General)
Online Access:http://umpir.ump.edu.my/id/eprint/34064/1/A%20new%20L-glutaminase%20from%20Kosakonia%20sp..pdf
http://umpir.ump.edu.my/id/eprint/34064/
https://doi.org/10.1007/s11694-020-00682-z
https://doi.org/10.1007/s11694-020-00682-z
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spelling my.ump.umpir.340642022-05-12T02:37:51Z http://umpir.ump.edu.my/id/eprint/34064/ A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis Aizi Nor Mazila, Ramli Nur Dini, Johari Mohd Akmal, Azhar Rohaida, Che Man Hazrulrizawati, Hamid Q Science (General) A comprehensive research has been carried out on L-glutaminase producers from marine bacteria. Among the evaluated isolates, Kosakonia radicincitans was found to be a L-glutaminase producer from a marine environment, having the highest enzymatic activities. The optimal condition for L-glutaminase production from K. radicincitans was at pH and temperature of 7.0 and 37 °C, respectively. The enzyme was found stable between pH 4 and 8 and its function also maintained at temperatures between 31 and 43 °C after 30-min incubation. Both the amino acid sequence and domain analysis of the L-glutaminase from the Kosakonia strains revealed various similarities and dissimilarities between these amidase enzymes. Sequence analysis and comparison of L-glutaminase from Kosakonia sp. with other L-glutaminase along with structural prediction were able to identify the unique characteristics of the predicted structures and offer a different perspective into their distinguishable properties for industrial use. Springer International Publishing 2021 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/34064/1/A%20new%20L-glutaminase%20from%20Kosakonia%20sp..pdf Aizi Nor Mazila, Ramli and Nur Dini, Johari and Mohd Akmal, Azhar and Rohaida, Che Man and Hazrulrizawati, Hamid (2021) A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis. Journal of Food Measurement and Characterization, 15. pp. 862-875. ISSN 2193-4126 (Print); 2193-4134 (Online). (Published) https://doi.org/10.1007/s11694-020-00682-z https://doi.org/10.1007/s11694-020-00682-z
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Aizi Nor Mazila, Ramli
Nur Dini, Johari
Mohd Akmal, Azhar
Rohaida, Che Man
Hazrulrizawati, Hamid
A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis
description A comprehensive research has been carried out on L-glutaminase producers from marine bacteria. Among the evaluated isolates, Kosakonia radicincitans was found to be a L-glutaminase producer from a marine environment, having the highest enzymatic activities. The optimal condition for L-glutaminase production from K. radicincitans was at pH and temperature of 7.0 and 37 °C, respectively. The enzyme was found stable between pH 4 and 8 and its function also maintained at temperatures between 31 and 43 °C after 30-min incubation. Both the amino acid sequence and domain analysis of the L-glutaminase from the Kosakonia strains revealed various similarities and dissimilarities between these amidase enzymes. Sequence analysis and comparison of L-glutaminase from Kosakonia sp. with other L-glutaminase along with structural prediction were able to identify the unique characteristics of the predicted structures and offer a different perspective into their distinguishable properties for industrial use.
format Article
author Aizi Nor Mazila, Ramli
Nur Dini, Johari
Mohd Akmal, Azhar
Rohaida, Che Man
Hazrulrizawati, Hamid
author_facet Aizi Nor Mazila, Ramli
Nur Dini, Johari
Mohd Akmal, Azhar
Rohaida, Che Man
Hazrulrizawati, Hamid
author_sort Aizi Nor Mazila, Ramli
title A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis
title_short A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis
title_full A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis
title_fullStr A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis
title_full_unstemmed A new L-glutaminase from Kosakonia sp.: extracellular production, gene identification and structural analysis
title_sort new l-glutaminase from kosakonia sp.: extracellular production, gene identification and structural analysis
publisher Springer International Publishing
publishDate 2021
url http://umpir.ump.edu.my/id/eprint/34064/1/A%20new%20L-glutaminase%20from%20Kosakonia%20sp..pdf
http://umpir.ump.edu.my/id/eprint/34064/
https://doi.org/10.1007/s11694-020-00682-z
https://doi.org/10.1007/s11694-020-00682-z
_version_ 1822922599937605632
score 13.252575

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