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Extraction of papain enzyme from papaya leaves using enzyme assisted method

Papain is highly appreciated in medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. Therefore, the objective of the research is to extract papain enzyme from papaya leaves using enzyme assisted method and determine papain activity. The Cellullase...

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Main Author: Roswani, Shaimi
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/3323/1/CD5512_ROSWANI_SHAIMI_i.pdf
http://umpir.ump.edu.my/id/eprint/3323/
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spelling my.ump.umpir.33232015-03-03T08:00:33Z http://umpir.ump.edu.my/id/eprint/3323/ Extraction of papain enzyme from papaya leaves using enzyme assisted method Roswani, Shaimi TP Chemical technology Papain is highly appreciated in medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. Therefore, the objective of the research is to extract papain enzyme from papaya leaves using enzyme assisted method and determine papain activity. The Cellullase assisted extraction process was optimized by varying different parameters such as pH of extraction process, Cellulase concentration, Solid to Liquid ratio, incubation time and incubation temperature. Each 5 g of papaya leaves were ground and mixed with acetate buffer at a different Solid to Liquid ratio (1:5 - 1:25 g/ml) and adjusted with different pH (3-8). Cellulase was quantified and dispersed in acetate buffer (Na2HPO—citric acid) in different concentration (0.5 - 2.5 mg/ml).The enzymatic pretreatment was carried out and continued for enzyme assisted extraction process for various temperature and time (5070o4C and 1-5 h respectively). The optimal extraction conditions that satisfied the above constraints were found to be at pH 7, 1.5 mg/ml of Cellulase, solid to liquid of 1:10 (g/ml), an extraction time of 4 h and at an extraction temperature of 65oC. Under the optimum conditions, the extraction yield of papain was successfully achieved as much as 3.8018 µmole with 2.0910 Units/ml in Cellulase treated sample. Therefore, the potential for the papain enzyme in medical field can be fulfilled using enzyme assisted extraction. 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3323/1/CD5512_ROSWANI_SHAIMI_i.pdf Roswani, Shaimi (2010) Extraction of papain enzyme from papaya leaves using enzyme assisted method. Faculty of Chemical and Natural Resources Engineering , Universiti Malaysia Pahang .
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Roswani, Shaimi
Extraction of papain enzyme from papaya leaves using enzyme assisted method
description Papain is highly appreciated in medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. Therefore, the objective of the research is to extract papain enzyme from papaya leaves using enzyme assisted method and determine papain activity. The Cellullase assisted extraction process was optimized by varying different parameters such as pH of extraction process, Cellulase concentration, Solid to Liquid ratio, incubation time and incubation temperature. Each 5 g of papaya leaves were ground and mixed with acetate buffer at a different Solid to Liquid ratio (1:5 - 1:25 g/ml) and adjusted with different pH (3-8). Cellulase was quantified and dispersed in acetate buffer (Na2HPO—citric acid) in different concentration (0.5 - 2.5 mg/ml).The enzymatic pretreatment was carried out and continued for enzyme assisted extraction process for various temperature and time (5070o4C and 1-5 h respectively). The optimal extraction conditions that satisfied the above constraints were found to be at pH 7, 1.5 mg/ml of Cellulase, solid to liquid of 1:10 (g/ml), an extraction time of 4 h and at an extraction temperature of 65oC. Under the optimum conditions, the extraction yield of papain was successfully achieved as much as 3.8018 µmole with 2.0910 Units/ml in Cellulase treated sample. Therefore, the potential for the papain enzyme in medical field can be fulfilled using enzyme assisted extraction.
format Undergraduates Project Papers
author Roswani, Shaimi
author_facet Roswani, Shaimi
author_sort Roswani, Shaimi
title Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_short Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_full Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_fullStr Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_full_unstemmed Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_sort extraction of papain enzyme from papaya leaves using enzyme assisted method
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/3323/1/CD5512_ROSWANI_SHAIMI_i.pdf
http://umpir.ump.edu.my/id/eprint/3323/
_version_ 1643664783230107648
score 13.211869

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