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Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis

Recombinant enzyme excretion is essential due to stable and active protein production. However, enzyme excretion, especially in the Escherichia coli system, may increase the occurrence of cell lysis. Thus, cell immobilization was used, and optimization of culture conditions was conducted to increase...

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Main Authors:	Rohaida, Che Man, Rosli, Md Illias, Aizi Nor Mazila, Ramli, Siti Kholijah, Abdul Mudalip
Format:	Article
Language:	English English
Published:	Wiley 2022
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/33066/1/17.%20Optimization%20of%20the%20cultural%20conditions%20of%20immobilized%20cells%20for%20enzyme%20excretion%20and%20cell%20lysis.pdf http://umpir.ump.edu.my/id/eprint/33066/2/17.1%20Optimization%20of%20the%20cultural%20conditions%20of%20immobilized%20cells%20for%20enzyme%20excretion%20and%20cell%20lysis.pdf http://umpir.ump.edu.my/id/eprint/33066/ https://doi.org/10.1002/ceat.202100425 https://doi.org/10.1002/ceat.202100425
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spelling	my.ump.umpir.330662023-04-19T01:40:50Z http://umpir.ump.edu.my/id/eprint/33066/ Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis Rohaida, Che Man Rosli, Md Illias Aizi Nor Mazila, Ramli Siti Kholijah, Abdul Mudalip TP Chemical technology Recombinant enzyme excretion is essential due to stable and active protein production. However, enzyme excretion, especially in the Escherichia coli system, may increase the occurrence of cell lysis. Thus, cell immobilization was used, and optimization of culture conditions was conducted to increase enzyme excretion and limit cell lysis. The culture conditions were optimized using a response surface methodology. CGTase excretion and cell lysis were analyzed by CGTase and β-galactosidase assays, respectively. High CGTase excretion with low cell lysis was achieved after the optimization process. The results suggest that optimization of the cell immobilization system increased CGTase excretion with low cell lysis and made it more valuable for industrial applications. Wiley 2022 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/33066/1/17.%20Optimization%20of%20the%20cultural%20conditions%20of%20immobilized%20cells%20for%20enzyme%20excretion%20and%20cell%20lysis.pdf pdf en http://umpir.ump.edu.my/id/eprint/33066/2/17.1%20Optimization%20of%20the%20cultural%20conditions%20of%20immobilized%20cells%20for%20enzyme%20excretion%20and%20cell%20lysis.pdf Rohaida, Che Man and Rosli, Md Illias and Aizi Nor Mazila, Ramli and Siti Kholijah, Abdul Mudalip (2022) Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis. Chemical Engineering and Technology, 45 (8). 1461 -1466. ISSN 1521-4125 https://doi.org/10.1002/ceat.202100425 https://doi.org/10.1002/ceat.202100425
institution	Universiti Malaysia Pahang
building	UMP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Pahang
content_source	UMP Institutional Repository
url_provider	http://umpir.ump.edu.my/
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rohaida, Che Man Rosli, Md Illias Aizi Nor Mazila, Ramli Siti Kholijah, Abdul Mudalip Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis
description	Recombinant enzyme excretion is essential due to stable and active protein production. However, enzyme excretion, especially in the Escherichia coli system, may increase the occurrence of cell lysis. Thus, cell immobilization was used, and optimization of culture conditions was conducted to increase enzyme excretion and limit cell lysis. The culture conditions were optimized using a response surface methodology. CGTase excretion and cell lysis were analyzed by CGTase and β-galactosidase assays, respectively. High CGTase excretion with low cell lysis was achieved after the optimization process. The results suggest that optimization of the cell immobilization system increased CGTase excretion with low cell lysis and made it more valuable for industrial applications.
format	Article
author	Rohaida, Che Man Rosli, Md Illias Aizi Nor Mazila, Ramli Siti Kholijah, Abdul Mudalip
author_facet	Rohaida, Che Man Rosli, Md Illias Aizi Nor Mazila, Ramli Siti Kholijah, Abdul Mudalip
author_sort	Rohaida, Che Man
title	Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis
title_short	Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis
title_full	Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis
title_fullStr	Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis
title_full_unstemmed	Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis
title_sort	optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis
publisher	Wiley
publishDate	2022
url	http://umpir.ump.edu.my/id/eprint/33066/1/17.%20Optimization%20of%20the%20cultural%20conditions%20of%20immobilized%20cells%20for%20enzyme%20excretion%20and%20cell%20lysis.pdf http://umpir.ump.edu.my/id/eprint/33066/2/17.1%20Optimization%20of%20the%20cultural%20conditions%20of%20immobilized%20cells%20for%20enzyme%20excretion%20and%20cell%20lysis.pdf http://umpir.ump.edu.my/id/eprint/33066/ https://doi.org/10.1002/ceat.202100425 https://doi.org/10.1002/ceat.202100425
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score	13.211869

Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis

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