Optimization of extraction of antioxidants from lamiaceae sp. by boiling

This research is conducted to study the effects of temperature and time of boiling in the extraction of antioxidants from ati-ati (Lamiaceae sp.) plants. In this research, there are two major objectives which are the study of the effect on antioxidants activity of ati-ati (Lamiaceae sp.) plants extr...

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Main Author: Muhammad Affiq Saufi, Nordin
Format: Undergraduates Project Papers
Language:English
Published: 2010
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Online Access:http://umpir.ump.edu.my/id/eprint/3186/1/CD5588_MUHAMMAD_AFFIQ_SAUFI_NORDIN.pdf
http://umpir.ump.edu.my/id/eprint/3186/
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spelling my.ump.umpir.31862021-07-05T03:33:49Z http://umpir.ump.edu.my/id/eprint/3186/ Optimization of extraction of antioxidants from lamiaceae sp. by boiling Muhammad Affiq Saufi, Nordin TP Chemical technology This research is conducted to study the effects of temperature and time of boiling in the extraction of antioxidants from ati-ati (Lamiaceae sp.) plants. In this research, there are two major objectives which are the study of the effect on antioxidants activity of ati-ati (Lamiaceae sp.) plants extraction conducted by repeated boiling and the study of scale up and optimization of the antioxidants extraction from ati-ati (Lamiaceae sp.) plants. In the scale up and optimization study, the experiments were conducted under different levels of two variables of temperature (50, 75 and 100 oC) and time (60, 105 and 150 minutes). In the repeated boiling study, the levels of temperature and time used are at the optimum condition only which are at 100 oC and 150 minutes. The method used to analyze antioxidants levels was 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay. Antioxidants activity assay are based on measurement of the loss of DPPH colour at wavelength of 517 nm monitored via UV-VIS spectrophotometers. The percentage of the DPPH remaining is calculated as % antioxidant activity. In order to determine the optimum condition of the antioxidants activity, several mathematical method were used such as the 22 factorial experiments and using linear regression. In the use of Yates‟ method, the calculation has proved that temperature has contributed to the biggest main effect on antioxidants activity while the interactive effect of temperature and time on antioxidant yield is also significant. For the linear regression method, the coefficients a₁ to a₂ that are obtained from calculation are small compared to the constant a₀. The area investigated is a plateau which may contain the maximum yield of antioxidants activity. 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3186/1/CD5588_MUHAMMAD_AFFIQ_SAUFI_NORDIN.pdf Muhammad Affiq Saufi, Nordin (2010) Optimization of extraction of antioxidants from lamiaceae sp. by boiling. Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang .
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Muhammad Affiq Saufi, Nordin
Optimization of extraction of antioxidants from lamiaceae sp. by boiling
description This research is conducted to study the effects of temperature and time of boiling in the extraction of antioxidants from ati-ati (Lamiaceae sp.) plants. In this research, there are two major objectives which are the study of the effect on antioxidants activity of ati-ati (Lamiaceae sp.) plants extraction conducted by repeated boiling and the study of scale up and optimization of the antioxidants extraction from ati-ati (Lamiaceae sp.) plants. In the scale up and optimization study, the experiments were conducted under different levels of two variables of temperature (50, 75 and 100 oC) and time (60, 105 and 150 minutes). In the repeated boiling study, the levels of temperature and time used are at the optimum condition only which are at 100 oC and 150 minutes. The method used to analyze antioxidants levels was 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay. Antioxidants activity assay are based on measurement of the loss of DPPH colour at wavelength of 517 nm monitored via UV-VIS spectrophotometers. The percentage of the DPPH remaining is calculated as % antioxidant activity. In order to determine the optimum condition of the antioxidants activity, several mathematical method were used such as the 22 factorial experiments and using linear regression. In the use of Yates‟ method, the calculation has proved that temperature has contributed to the biggest main effect on antioxidants activity while the interactive effect of temperature and time on antioxidant yield is also significant. For the linear regression method, the coefficients a₁ to a₂ that are obtained from calculation are small compared to the constant a₀. The area investigated is a plateau which may contain the maximum yield of antioxidants activity.
format Undergraduates Project Papers
author Muhammad Affiq Saufi, Nordin
author_facet Muhammad Affiq Saufi, Nordin
author_sort Muhammad Affiq Saufi, Nordin
title Optimization of extraction of antioxidants from lamiaceae sp. by boiling
title_short Optimization of extraction of antioxidants from lamiaceae sp. by boiling
title_full Optimization of extraction of antioxidants from lamiaceae sp. by boiling
title_fullStr Optimization of extraction of antioxidants from lamiaceae sp. by boiling
title_full_unstemmed Optimization of extraction of antioxidants from lamiaceae sp. by boiling
title_sort optimization of extraction of antioxidants from lamiaceae sp. by boiling
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/3186/1/CD5588_MUHAMMAD_AFFIQ_SAUFI_NORDIN.pdf
http://umpir.ump.edu.my/id/eprint/3186/
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score 13.211869