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Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods

Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyde...

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Main Authors: Abdi Wira, Septama, Ibrahim, Jantan, Panichayupakaranant, Pharkphoom, Mohd F. F., Mohd Aluwi, Eldiza Puji, Rahmi
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2020
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/30864/1/Immunosuppressive%20and%20antibacterial%20activities%20of%20dihydromorin%20and%20norartocarpetin.pdf
http://umpir.ump.edu.my/id/eprint/30864/
https://doi.org/10.4103/2221-1691.287162
https://doi.org/10.4103/2221-1691.287162
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spelling my.ump.umpir.308642021-06-30T14:21:15Z http://umpir.ump.edu.my/id/eprint/30864/ Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods Abdi Wira, Septama Ibrahim, Jantan Panichayupakaranant, Pharkphoom Mohd F. F., Mohd Aluwi Eldiza Puji, Rahmi TP Chemical technology Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyden chamber was used to determine the chemotactic activity of human phagocyte. The respiratory burst was evaluated by chemiluminescence assay. Myeloperoxidase (MPO) activity was quantified using a colorimetric assay. The broth microdilution method was performed to assess their antibacterial activity. Results: Dihydromorin exhibited potent inhibitory effect on the chemotactic activity of polymorphonuclear neutrophils (PMNs) with an IC50 value of 5.03 μg/mL. Dihydromorin also inhibited reactive oxygen species production of whole blood cells, PMNs, and monocytes with IC50 values of 7.88, 7.59 and 7.24 μg/mL, respectively. Interestingly, dihydromorin also strongly inhibited the MPO activity of PMNs with an IC50 value of 5.24 μg/mL, which was lower than indomethacin (24.6 μg/mL). Molecular docking of dihydromorin and crystal structure of MPO showed that dihydromorin had close interaction with key amino acid residues such as Arg239 and Gln91. Antibacterial activity assay showed that only dihydromorin had a strong effect against Streptococcus pyogenes with MIC and MBC values of 15.62 and 31.25 μg/mL, respectively. Conclusions: The results suggest that dihydromorin could be developed as an anti-inflammatory and antibacterial agent. Wolters Kluwer Medknow Publications 2020-08-01 Article PeerReviewed pdf en cc_by_nc_sa_4 http://umpir.ump.edu.my/id/eprint/30864/1/Immunosuppressive%20and%20antibacterial%20activities%20of%20dihydromorin%20and%20norartocarpetin.pdf Abdi Wira, Septama and Ibrahim, Jantan and Panichayupakaranant, Pharkphoom and Mohd F. F., Mohd Aluwi and Eldiza Puji, Rahmi (2020) Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods. Asian Pacific Journal of Tropical Biomedicine, 10 (8). pp. 361-368. ISSN 2221-1691 https://doi.org/10.4103/2221-1691.287162 https://doi.org/10.4103/2221-1691.287162
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Abdi Wira, Septama
Ibrahim, Jantan
Panichayupakaranant, Pharkphoom
Mohd F. F., Mohd Aluwi
Eldiza Puji, Rahmi
Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods
description Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyden chamber was used to determine the chemotactic activity of human phagocyte. The respiratory burst was evaluated by chemiluminescence assay. Myeloperoxidase (MPO) activity was quantified using a colorimetric assay. The broth microdilution method was performed to assess their antibacterial activity. Results: Dihydromorin exhibited potent inhibitory effect on the chemotactic activity of polymorphonuclear neutrophils (PMNs) with an IC50 value of 5.03 μg/mL. Dihydromorin also inhibited reactive oxygen species production of whole blood cells, PMNs, and monocytes with IC50 values of 7.88, 7.59 and 7.24 μg/mL, respectively. Interestingly, dihydromorin also strongly inhibited the MPO activity of PMNs with an IC50 value of 5.24 μg/mL, which was lower than indomethacin (24.6 μg/mL). Molecular docking of dihydromorin and crystal structure of MPO showed that dihydromorin had close interaction with key amino acid residues such as Arg239 and Gln91. Antibacterial activity assay showed that only dihydromorin had a strong effect against Streptococcus pyogenes with MIC and MBC values of 15.62 and 31.25 μg/mL, respectively. Conclusions: The results suggest that dihydromorin could be developed as an anti-inflammatory and antibacterial agent.
format Article
author Abdi Wira, Septama
Ibrahim, Jantan
Panichayupakaranant, Pharkphoom
Mohd F. F., Mohd Aluwi
Eldiza Puji, Rahmi
author_facet Abdi Wira, Septama
Ibrahim, Jantan
Panichayupakaranant, Pharkphoom
Mohd F. F., Mohd Aluwi
Eldiza Puji, Rahmi
author_sort Abdi Wira, Septama
title Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods
title_short Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods
title_full Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods
title_fullStr Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods
title_full_unstemmed Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods
title_sort immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods
publisher Wolters Kluwer Medknow Publications
publishDate 2020
url http://umpir.ump.edu.my/id/eprint/30864/1/Immunosuppressive%20and%20antibacterial%20activities%20of%20dihydromorin%20and%20norartocarpetin.pdf
http://umpir.ump.edu.my/id/eprint/30864/
https://doi.org/10.4103/2221-1691.287162
https://doi.org/10.4103/2221-1691.287162
_version_ 1705057018663927808
score 13.211869

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