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Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis

A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL...

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Main Authors: Nur Husnina Iffah, Bakar, Thong, Chuan Lee, Mohd Hairul, Ab. Rahim, Raha, Ahmad Raus, Dayang Fredalina, Basri, Noor Suhana, Adzahar
Format: Article
Language:English
Published: Trans Tech Publications 2020
Subjects:
T Technology (General)
Online Access:http://umpir.ump.edu.my/id/eprint/30520/1/12.1%20Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis.pdf
http://umpir.ump.edu.my/id/eprint/30520/
https://doi.org/10.4028/www.scientific.net/MSF.981.265
https://doi.org/10.4028/www.scientific.net/MSF.981.265
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spelling my.ump.umpir.305202021-02-08T03:45:40Z http://umpir.ump.edu.my/id/eprint/30520/ Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis Nur Husnina Iffah, Bakar Thong, Chuan Lee Mohd Hairul, Ab. Rahim Raha, Ahmad Raus Dayang Fredalina, Basri Noor Suhana, Adzahar T Technology (General) A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract. Trans Tech Publications 2020 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/30520/1/12.1%20Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis.pdf Nur Husnina Iffah, Bakar and Thong, Chuan Lee and Mohd Hairul, Ab. Rahim and Raha, Ahmad Raus and Dayang Fredalina, Basri and Noor Suhana, Adzahar (2020) Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis. Materials Science Forum, 981. pp. 265-270. ISSN 0255-5476 https://doi.org/10.4028/www.scientific.net/MSF.981.265 https://doi.org/10.4028/www.scientific.net/MSF.981.265
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Nur Husnina Iffah, Bakar
Thong, Chuan Lee
Mohd Hairul, Ab. Rahim
Raha, Ahmad Raus
Dayang Fredalina, Basri
Noor Suhana, Adzahar
Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
description A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract.
format Article
author Nur Husnina Iffah, Bakar
Thong, Chuan Lee
Mohd Hairul, Ab. Rahim
Raha, Ahmad Raus
Dayang Fredalina, Basri
Noor Suhana, Adzahar
author_facet Nur Husnina Iffah, Bakar
Thong, Chuan Lee
Mohd Hairul, Ab. Rahim
Raha, Ahmad Raus
Dayang Fredalina, Basri
Noor Suhana, Adzahar
author_sort Nur Husnina Iffah, Bakar
title Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_short Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_full Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_fullStr Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_full_unstemmed Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis
title_sort real-time cytotoxicity assay of water extracts hibiscus rosa-sinensis
publisher Trans Tech Publications
publishDate 2020
url http://umpir.ump.edu.my/id/eprint/30520/1/12.1%20Real-time%20cytotoxicity%20assay%20of%20water%20extracts%20Hibiscus%20rosa-sinensis.pdf
http://umpir.ump.edu.my/id/eprint/30520/
https://doi.org/10.4028/www.scientific.net/MSF.981.265
https://doi.org/10.4028/www.scientific.net/MSF.981.265
_version_ 1691733262782693376
score 13.211869

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