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Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA)

Twelve probiotic Lactobacillus strains for poultry were characterised by amplified ribosomal DNA restriction analysis (ARDRA) using Sau3AI, TaqI, HaeIII and AluI restriction endonucleases. Species-specific and strain-specific restriction patterns were observed from the bacterial strains. Numerical a...

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Main Authors: Lee, Chin Mei, Sieo, Chin Chin, Norhani, Abdullah, Ho, Yin Wan
Format: Conference or Workshop Item
Language:English
English
Published: Trans Tech Publications, Switzerland 2020
Subjects:
TA Engineering (General). Civil engineering (General)
Online Access:http://umpir.ump.edu.my/id/eprint/30474/1/5.1%20Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified.pdf
http://umpir.ump.edu.my/id/eprint/30474/7/Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified%20ribosomal%20dna%20restriction%20analysis.pdf
http://umpir.ump.edu.my/id/eprint/30474/
https://doi.org/10.4028/www.scientific.net/MSF.981.302
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spelling my.ump.umpir.304742021-02-26T03:25:54Z http://umpir.ump.edu.my/id/eprint/30474/ Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA) Lee, Chin Mei Sieo, Chin Chin Norhani, Abdullah Ho, Yin Wan TA Engineering (General). Civil engineering (General) Twelve probiotic Lactobacillus strains for poultry were characterised by amplified ribosomal DNA restriction analysis (ARDRA) using Sau3AI, TaqI, HaeIII and AluI restriction endonucleases. Species-specific and strain-specific restriction patterns were observed from the bacterial strains. Numerical analysis of composite analysis of ARDRA exhibited D value of 0.8456. Whereas, the caculated D values of ARDRA patterns generated by Sau3AI, TaqI, HaeIII and AluI were 0.8309, 0.8382,0.8088 and 0.8088, repectively. Composite analysis of ARDRA was the most discriminative method when compared to the individual analysis. ARDRA could distinguished L. reuteri C 10 and L. panis C 17 into single strains. The 16S rRNA gene restriction patterns were also able to group L. gallinarum I 16 and I 26 into single strains. Lactobacillus brevis I 12, I 23, I 25, I 211 and I 218 seem to be multiple clones of the same bacterial strain as are L. reuteri C 1 and C 16. ARDRA is a valuable fingerprinting method to discriminate probiotic Lactobacillus strains. Trans Tech Publications, Switzerland 2020 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/30474/1/5.1%20Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified.pdf pdf en http://umpir.ump.edu.my/id/eprint/30474/7/Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified%20ribosomal%20dna%20restriction%20analysis.pdf Lee, Chin Mei and Sieo, Chin Chin and Norhani, Abdullah and Ho, Yin Wan (2020) Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA). In: Symposium on Industrial Science and Technology, SISTEC 2019, 22-23 Aug. 2019 , Kuantan, Malaysia. pp. 302-308., 981. ISSN 1662-9752 https://doi.org/10.4028/www.scientific.net/MSF.981.302
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
topic TA Engineering (General). Civil engineering (General)
spellingShingle TA Engineering (General). Civil engineering (General)
Lee, Chin Mei
Sieo, Chin Chin
Norhani, Abdullah
Ho, Yin Wan
Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA)
description Twelve probiotic Lactobacillus strains for poultry were characterised by amplified ribosomal DNA restriction analysis (ARDRA) using Sau3AI, TaqI, HaeIII and AluI restriction endonucleases. Species-specific and strain-specific restriction patterns were observed from the bacterial strains. Numerical analysis of composite analysis of ARDRA exhibited D value of 0.8456. Whereas, the caculated D values of ARDRA patterns generated by Sau3AI, TaqI, HaeIII and AluI were 0.8309, 0.8382,0.8088 and 0.8088, repectively. Composite analysis of ARDRA was the most discriminative method when compared to the individual analysis. ARDRA could distinguished L. reuteri C 10 and L. panis C 17 into single strains. The 16S rRNA gene restriction patterns were also able to group L. gallinarum I 16 and I 26 into single strains. Lactobacillus brevis I 12, I 23, I 25, I 211 and I 218 seem to be multiple clones of the same bacterial strain as are L. reuteri C 1 and C 16. ARDRA is a valuable fingerprinting method to discriminate probiotic Lactobacillus strains.
format Conference or Workshop Item
author Lee, Chin Mei
Sieo, Chin Chin
Norhani, Abdullah
Ho, Yin Wan
author_facet Lee, Chin Mei
Sieo, Chin Chin
Norhani, Abdullah
Ho, Yin Wan
author_sort Lee, Chin Mei
title Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA)
title_short Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA)
title_full Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA)
title_fullStr Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA)
title_full_unstemmed Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA)
title_sort characterisations of probiotic lactobacillus strains by amplified ribosomal dna restriction analysis (ardra)
publisher Trans Tech Publications, Switzerland
publishDate 2020
url http://umpir.ump.edu.my/id/eprint/30474/1/5.1%20Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified.pdf
http://umpir.ump.edu.my/id/eprint/30474/7/Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified%20ribosomal%20dna%20restriction%20analysis.pdf
http://umpir.ump.edu.my/id/eprint/30474/
https://doi.org/10.4028/www.scientific.net/MSF.981.302
_version_ 1692991964933259264
score 13.211869

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