Cover Image

Characterization of aquilaria malaccensis callus cells using SEM and somatic embryogenesis associated genes identification

Aquilaria malaccensis or gaharu is one of Malaysia's top tropical resources that is being protected and conserved. Somatic embryogenesis is a plant tissue culture method used most extensively in commercial micropropagation systems and conservation purposes. This study aims to optimize callus in...

Full description

Saved in:
Bibliographic Details
Main Authors: Mohd Syafik, Mohamad Hamdan, Aimi Wahidah, Aminan, Siti Hajar Noor, Shaarani, Saiful Nizam, Tajuddin
Format: Conference or Workshop Item
Language:English
Published: IOP Publishing Ltd. 2020
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/29918/1/Characterization%20of%20aquilaria%20malaccensis%20callus%20cells%20using%20SEM.pdf
http://umpir.ump.edu.my/id/eprint/29918/
https://doi.org/10.1088/1755-1315/549/1/012070
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ump.umpir.29918
record_format eprints
spelling my.ump.umpir.299182021-04-02T07:25:43Z http://umpir.ump.edu.my/id/eprint/29918/ Characterization of aquilaria malaccensis callus cells using SEM and somatic embryogenesis associated genes identification Mohd Syafik, Mohamad Hamdan Aimi Wahidah, Aminan Siti Hajar Noor, Shaarani Saiful Nizam, Tajuddin TP Chemical technology Aquilaria malaccensis or gaharu is one of Malaysia's top tropical resources that is being protected and conserved. Somatic embryogenesis is a plant tissue culture method used most extensively in commercial micropropagation systems and conservation purposes. This study aims to optimize callus induction techniques for somatic embryogenesis study using Scanning Electron Microscope (SEM) and Polymerase Chain Reaction (PCR) analysis. In this study, callus was induced on Murashige and Skoog's (MS) medium supplemented with a combination of plant growth regulators and optimized parameters (explant used, sterilization techniques, media content, pH media, plant growth regulators, incubation condition, and incubation temperature). The cells were subcultured for long-term callus maintenance and subjected to SEM analysis for somatic embryogenesis confirmation. Four set of genes associated to somatic embryogenesis (SERK, BBM, LEC1, and WOX) were studied based on National Centre for Biotechnology Information database and literatures. The data obtained were used for primer design and gene amplification using 3 different tissues (leaf, stem, callus).The highest frequencies of callus induction were observed on Murashikage and Skoog medium supplemented with 6-Benzylaminopurine and 1-Naphthaleneacetic acid together with optimized growth parameters. SEM analysis showed embryogenic characteristics in cells of the yellow compact calli evidenced by the presence of small and isodiametric cells. Only SERK gene was successfully amplified and enable to proceed with in silico analysis. This study provides fundamental results for genetic conservation effort of A. malaccensis related to somatic embryogenesis study. IOP Publishing Ltd. 2020-09-22 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/29918/1/Characterization%20of%20aquilaria%20malaccensis%20callus%20cells%20using%20SEM.pdf Mohd Syafik, Mohamad Hamdan and Aimi Wahidah, Aminan and Siti Hajar Noor, Shaarani and Saiful Nizam, Tajuddin (2020) Characterization of aquilaria malaccensis callus cells using SEM and somatic embryogenesis associated genes identification. In: IOP Conference Series: Earth and Environmental Science, 2nd International Conference on Tropical Resources and Sustainable Sciences (CTReSS 2020), 10-11 August 2020 , Universiti Malaysia Kelantan, City Campus, Kelantan. pp. 1-12., 549 (012070). ISSN 1755-1307 https://doi.org/10.1088/1755-1315/549/1/012070
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mohd Syafik, Mohamad Hamdan
Aimi Wahidah, Aminan
Siti Hajar Noor, Shaarani
Saiful Nizam, Tajuddin
Characterization of aquilaria malaccensis callus cells using SEM and somatic embryogenesis associated genes identification
description Aquilaria malaccensis or gaharu is one of Malaysia's top tropical resources that is being protected and conserved. Somatic embryogenesis is a plant tissue culture method used most extensively in commercial micropropagation systems and conservation purposes. This study aims to optimize callus induction techniques for somatic embryogenesis study using Scanning Electron Microscope (SEM) and Polymerase Chain Reaction (PCR) analysis. In this study, callus was induced on Murashige and Skoog's (MS) medium supplemented with a combination of plant growth regulators and optimized parameters (explant used, sterilization techniques, media content, pH media, plant growth regulators, incubation condition, and incubation temperature). The cells were subcultured for long-term callus maintenance and subjected to SEM analysis for somatic embryogenesis confirmation. Four set of genes associated to somatic embryogenesis (SERK, BBM, LEC1, and WOX) were studied based on National Centre for Biotechnology Information database and literatures. The data obtained were used for primer design and gene amplification using 3 different tissues (leaf, stem, callus).The highest frequencies of callus induction were observed on Murashikage and Skoog medium supplemented with 6-Benzylaminopurine and 1-Naphthaleneacetic acid together with optimized growth parameters. SEM analysis showed embryogenic characteristics in cells of the yellow compact calli evidenced by the presence of small and isodiametric cells. Only SERK gene was successfully amplified and enable to proceed with in silico analysis. This study provides fundamental results for genetic conservation effort of A. malaccensis related to somatic embryogenesis study.
format Conference or Workshop Item
author Mohd Syafik, Mohamad Hamdan
Aimi Wahidah, Aminan
Siti Hajar Noor, Shaarani
Saiful Nizam, Tajuddin
author_facet Mohd Syafik, Mohamad Hamdan
Aimi Wahidah, Aminan
Siti Hajar Noor, Shaarani
Saiful Nizam, Tajuddin
author_sort Mohd Syafik, Mohamad Hamdan
title Characterization of aquilaria malaccensis callus cells using SEM and somatic embryogenesis associated genes identification
title_short Characterization of aquilaria malaccensis callus cells using SEM and somatic embryogenesis associated genes identification
title_full Characterization of aquilaria malaccensis callus cells using SEM and somatic embryogenesis associated genes identification
title_fullStr Characterization of aquilaria malaccensis callus cells using SEM and somatic embryogenesis associated genes identification
title_full_unstemmed Characterization of aquilaria malaccensis callus cells using SEM and somatic embryogenesis associated genes identification
title_sort characterization of aquilaria malaccensis callus cells using sem and somatic embryogenesis associated genes identification
publisher IOP Publishing Ltd.
publishDate 2020
url http://umpir.ump.edu.my/id/eprint/29918/1/Characterization%20of%20aquilaria%20malaccensis%20callus%20cells%20using%20SEM.pdf
http://umpir.ump.edu.my/id/eprint/29918/
https://doi.org/10.1088/1755-1315/549/1/012070
_version_ 1696976425713664000
score 13.211869

Similar Items