Staff View: Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering

Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering

Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-"-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loade...

Full description

Saved in:

Bibliographic Details
Main Authors:	Hariharan, Ezhilarasu, Raghavendra, Ramalingam, Chetna, Dhand, Lakshminarayanan, Rajamani, Asif, Sadiq, Gandhimathi, Chinnasamy, Seeram, Ramakrishna, Boon, Huat Bay, Venugopal, Jayarama Reddy, Dinesh Kumar, Srinivasan
Format:	Article
Language:	English
Published:	MDPI 2019
Subjects:	RL Dermatology
Online Access:	http://umpir.ump.edu.my/id/eprint/29674/1/Int%20J%20Mol%20Sci%202019%2020%205174.pdf http://umpir.ump.edu.my/id/eprint/29674/ https://www.mdpi.com/journal/ijms http://dx.doi.org/10.3390/ijms20205174
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.ump.umpir.29674
record_format	eprints
spelling	my.ump.umpir.296742020-10-22T07:25:27Z http://umpir.ump.edu.my/id/eprint/29674/ Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering Hariharan, Ezhilarasu Raghavendra, Ramalingam Chetna, Dhand Lakshminarayanan, Rajamani Asif, Sadiq Gandhimathi, Chinnasamy Seeram, Ramakrishna Boon, Huat Bay Venugopal, Jayarama Reddy Dinesh Kumar, Srinivasan RL Dermatology Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-"-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc disffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering. MDPI 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/29674/1/Int%20J%20Mol%20Sci%202019%2020%205174.pdf Hariharan, Ezhilarasu and Raghavendra, Ramalingam and Chetna, Dhand and Lakshminarayanan, Rajamani and Asif, Sadiq and Gandhimathi, Chinnasamy and Seeram, Ramakrishna and Boon, Huat Bay and Venugopal, Jayarama Reddy and Dinesh Kumar, Srinivasan (2019) Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering. International Journal of Molecular Science, 20 (5174). pp. 1-16. https://www.mdpi.com/journal/ijms http://dx.doi.org/10.3390/ijms20205174
institution	Universiti Malaysia Pahang
building	UMP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Pahang
content_source	UMP Institutional Repository
url_provider	http://umpir.ump.edu.my/
language	English
topic	RL Dermatology
spellingShingle	RL Dermatology Hariharan, Ezhilarasu Raghavendra, Ramalingam Chetna, Dhand Lakshminarayanan, Rajamani Asif, Sadiq Gandhimathi, Chinnasamy Seeram, Ramakrishna Boon, Huat Bay Venugopal, Jayarama Reddy Dinesh Kumar, Srinivasan Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering
description	Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-"-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc disffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering.
format	Article
author	Hariharan, Ezhilarasu Raghavendra, Ramalingam Chetna, Dhand Lakshminarayanan, Rajamani Asif, Sadiq Gandhimathi, Chinnasamy Seeram, Ramakrishna Boon, Huat Bay Venugopal, Jayarama Reddy Dinesh Kumar, Srinivasan
author_facet	Hariharan, Ezhilarasu Raghavendra, Ramalingam Chetna, Dhand Lakshminarayanan, Rajamani Asif, Sadiq Gandhimathi, Chinnasamy Seeram, Ramakrishna Boon, Huat Bay Venugopal, Jayarama Reddy Dinesh Kumar, Srinivasan
author_sort	Hariharan, Ezhilarasu
title	Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering
title_short	Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering
title_full	Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering
title_fullStr	Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering
title_full_unstemmed	Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering
title_sort	biocompatible aloe vera and tetracycline hydrochloride loaded hybrid nanofibrous scaffolds for skin tissue engineering
publisher	MDPI
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/29674/1/Int%20J%20Mol%20Sci%202019%2020%205174.pdf http://umpir.ump.edu.my/id/eprint/29674/ https://www.mdpi.com/journal/ijms http://dx.doi.org/10.3390/ijms20205174
_version_	1681489643239899136
score	13.211869

Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering

Similar Items