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Surface modification of microcporous of polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility

Oil/water emulsion solvent evaporation method was employed to fabricate polycaprolactone (PCL) microcarriers. In order to produce porous microcarrier, the method was slightly modified. The porous network channels were generated inside the microcarrier by introducing camphene that was dissolved in th...

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Main Authors: Nurhusna, Samsudin, Yumi Z. H-Y., Hashim, Mohd Azmir, Arifin, Hamzah, M. Salleh
Format: Article
Language:English
Published: Indonesian Society for Knowledge and Human Development 2018
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/29464/1/Surface%20modification%20of%20microcporous%20of%20polycaprolactone.pdf
http://umpir.ump.edu.my/id/eprint/29464/
https://doi.org/10.18517/ijaseit.8.4-2.7060
https://doi.org/10.18517/ijaseit.8.4-2.7060
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spelling my.ump.umpir.294642022-11-09T03:00:38Z http://umpir.ump.edu.my/id/eprint/29464/ Surface modification of microcporous of polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility Nurhusna, Samsudin Yumi Z. H-Y., Hashim Mohd Azmir, Arifin Hamzah, M. Salleh TP Chemical technology Oil/water emulsion solvent evaporation method was employed to fabricate polycaprolactone (PCL) microcarriers. In order to produce porous microcarrier, the method was slightly modified. The porous network channels were generated inside the microcarrier by introducing camphene that was dissolved in the same solvent used to dissolve raw PCL. The evaporation of solvent and sublimination of camphene during the process of emulsion solvent evaporation method, produce solidified porous microcarrier beads. The surface porous microcarriers beads were further modified to make it competent for cell attachment and proliferation. The surface of these microcarriers were modified with UV/O3 system to introduce functional group and charge on the surface. The treatment followed with the immobilization of halal gelatin on the surface to imorove the biocompatibility of the microcarrier. Porous microcarrier with optimal size distribution was successfully fabricated. The average pore size of 11.74±8.32 µm was obtained with the concentration of 20% (w/v) of camphene. The porous PCL microcarrier was further tailored with gelatin through surface treatment with UV/O3 system and gelatin immobilization and validation of its compatibility towards mammalian cell was tested with cultivation of green monkyer kidney cell (Vero) in the suspension culture. Vero cells attach and proliferatie well on the gelatin coated porous PCL microcarrier with maximum cell number of 3.90 × 105 cells/ml as compared to cell proliferation on UV/O3 treated porous PCL microcarrier (1.83 × 105 cells/ml). The developed microcarrier may be potentially applicable as a cell delivery scaffold for cell tissue culture and tissue engineering application. Indonesian Society for Knowledge and Human Development 2018 Article PeerReviewed pdf en cc_by_sa_4 http://umpir.ump.edu.my/id/eprint/29464/1/Surface%20modification%20of%20microcporous%20of%20polycaprolactone.pdf Nurhusna, Samsudin and Yumi Z. H-Y., Hashim and Mohd Azmir, Arifin and Hamzah, M. Salleh (2018) Surface modification of microcporous of polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility. International Journal on Advanced Science, Engineering and Information Technology, 8 (4-2). pp. 1642-1647. ISSN 2088-5334 https://doi.org/10.18517/ijaseit.8.4-2.7060 https://doi.org/10.18517/ijaseit.8.4-2.7060
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nurhusna, Samsudin
Yumi Z. H-Y., Hashim
Mohd Azmir, Arifin
Hamzah, M. Salleh
Surface modification of microcporous of polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility
description Oil/water emulsion solvent evaporation method was employed to fabricate polycaprolactone (PCL) microcarriers. In order to produce porous microcarrier, the method was slightly modified. The porous network channels were generated inside the microcarrier by introducing camphene that was dissolved in the same solvent used to dissolve raw PCL. The evaporation of solvent and sublimination of camphene during the process of emulsion solvent evaporation method, produce solidified porous microcarrier beads. The surface porous microcarriers beads were further modified to make it competent for cell attachment and proliferation. The surface of these microcarriers were modified with UV/O3 system to introduce functional group and charge on the surface. The treatment followed with the immobilization of halal gelatin on the surface to imorove the biocompatibility of the microcarrier. Porous microcarrier with optimal size distribution was successfully fabricated. The average pore size of 11.74±8.32 µm was obtained with the concentration of 20% (w/v) of camphene. The porous PCL microcarrier was further tailored with gelatin through surface treatment with UV/O3 system and gelatin immobilization and validation of its compatibility towards mammalian cell was tested with cultivation of green monkyer kidney cell (Vero) in the suspension culture. Vero cells attach and proliferatie well on the gelatin coated porous PCL microcarrier with maximum cell number of 3.90 × 105 cells/ml as compared to cell proliferation on UV/O3 treated porous PCL microcarrier (1.83 × 105 cells/ml). The developed microcarrier may be potentially applicable as a cell delivery scaffold for cell tissue culture and tissue engineering application.
format Article
author Nurhusna, Samsudin
Yumi Z. H-Y., Hashim
Mohd Azmir, Arifin
Hamzah, M. Salleh
author_facet Nurhusna, Samsudin
Yumi Z. H-Y., Hashim
Mohd Azmir, Arifin
Hamzah, M. Salleh
author_sort Nurhusna, Samsudin
title Surface modification of microcporous of polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility
title_short Surface modification of microcporous of polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility
title_full Surface modification of microcporous of polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility
title_fullStr Surface modification of microcporous of polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility
title_full_unstemmed Surface modification of microcporous of polycaprolactone (PCL) microcarrier to improve microcarrier biocompatibility
title_sort surface modification of microcporous of polycaprolactone (pcl) microcarrier to improve microcarrier biocompatibility
publisher Indonesian Society for Knowledge and Human Development
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/29464/1/Surface%20modification%20of%20microcporous%20of%20polycaprolactone.pdf
http://umpir.ump.edu.my/id/eprint/29464/
https://doi.org/10.18517/ijaseit.8.4-2.7060
https://doi.org/10.18517/ijaseit.8.4-2.7060
_version_ 1751536360236253184
score 13.211869

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