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Protein denaturation in pilot scale spray dryer

The aim of this project is to investigate the protein denaturation in a pilot scale spray dryer. The occurrence of protein denaturation is due to external stresses, such as heat. Thus, this project describes a pilot process for obtaining protein isolates from whey protein in powder form via spray...

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Main Author: Kioh, Shin Fang
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/2696/1/KIOH_SHIN_FANG.PDF
http://umpir.ump.edu.my/id/eprint/2696/
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spelling my.ump.umpir.26962015-03-03T07:58:00Z http://umpir.ump.edu.my/id/eprint/2696/ Protein denaturation in pilot scale spray dryer Kioh, Shin Fang TP Chemical technology The aim of this project is to investigate the protein denaturation in a pilot scale spray dryer. The occurrence of protein denaturation is due to external stresses, such as heat. Thus, this project describes a pilot process for obtaining protein isolates from whey protein in powder form via spray drying process with improved water solubility and technofunctional properties as well as reduced thermal damage. The experiment, including varying the inlet air temperature at 100°C and 160°C as well as utilising the microencapsulation technique using a starch derived polysaccharide (or known as maltodextrin) to minimize the protein denaturation. The Reversed phase HPLC (RP-I-IPLC) technique has been applied to measure the denaturation of proteins in the samples which are with maltodextrin and without maltodextrin resulting from the spray drying of milk solution at inlet temperature of 100°C and 160°C. The combination of maltodextrin (MD) and fresh milk (FM) is at standard ratio of FM/MD (9: 1) in order to produce spherical and smooth powder. Apart from the RP-HPLC method, the SDS-PAGE method also used to analyze the protein content. The morphology of the milk powders was examined using a Scanning Electron Microscopy (SEM) while the particle size of milk products can be easily assessed using Mastersizer with model Scirocco 2000. To determine the portion of whey protein in the spray dried milk samples, the Total Solid Content method was employed. Besides, sterilization tests such as detection of Salmonella sp., and detection of Escherichia coli sp. along with microbial tests which is Standard Plate Count Method also applied. The spray-drying process was particularly critical for inducing some thermal damage but it is hypothesized that the crust formation which resulting in high particle temperatures while still maintaining a wet core, is likely to lead to high levels of denaturation up to 55% at high temperature (160 °C). Stability of a-lactalbumin proved higher as compare with fl-lactoglobulin. The study reveals that low outlet gas temperatures along with microencapsulation are required to avoid excessive denaturation of protein which can reduce the protein denaturation until 16%. Up to 93.2% reduction of the microorganisms also proved that spray drying is another form of sterilization. 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/2696/1/KIOH_SHIN_FANG.PDF Kioh, Shin Fang (2010) Protein denaturation in pilot scale spray dryer. FACULTY OF CHEMICAL & NATURAL RESOURCES ENGINEERING, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Kioh, Shin Fang
Protein denaturation in pilot scale spray dryer
description The aim of this project is to investigate the protein denaturation in a pilot scale spray dryer. The occurrence of protein denaturation is due to external stresses, such as heat. Thus, this project describes a pilot process for obtaining protein isolates from whey protein in powder form via spray drying process with improved water solubility and technofunctional properties as well as reduced thermal damage. The experiment, including varying the inlet air temperature at 100°C and 160°C as well as utilising the microencapsulation technique using a starch derived polysaccharide (or known as maltodextrin) to minimize the protein denaturation. The Reversed phase HPLC (RP-I-IPLC) technique has been applied to measure the denaturation of proteins in the samples which are with maltodextrin and without maltodextrin resulting from the spray drying of milk solution at inlet temperature of 100°C and 160°C. The combination of maltodextrin (MD) and fresh milk (FM) is at standard ratio of FM/MD (9: 1) in order to produce spherical and smooth powder. Apart from the RP-HPLC method, the SDS-PAGE method also used to analyze the protein content. The morphology of the milk powders was examined using a Scanning Electron Microscopy (SEM) while the particle size of milk products can be easily assessed using Mastersizer with model Scirocco 2000. To determine the portion of whey protein in the spray dried milk samples, the Total Solid Content method was employed. Besides, sterilization tests such as detection of Salmonella sp., and detection of Escherichia coli sp. along with microbial tests which is Standard Plate Count Method also applied. The spray-drying process was particularly critical for inducing some thermal damage but it is hypothesized that the crust formation which resulting in high particle temperatures while still maintaining a wet core, is likely to lead to high levels of denaturation up to 55% at high temperature (160 °C). Stability of a-lactalbumin proved higher as compare with fl-lactoglobulin. The study reveals that low outlet gas temperatures along with microencapsulation are required to avoid excessive denaturation of protein which can reduce the protein denaturation until 16%. Up to 93.2% reduction of the microorganisms also proved that spray drying is another form of sterilization.
format Undergraduates Project Papers
author Kioh, Shin Fang
author_facet Kioh, Shin Fang
author_sort Kioh, Shin Fang
title Protein denaturation in pilot scale spray dryer
title_short Protein denaturation in pilot scale spray dryer
title_full Protein denaturation in pilot scale spray dryer
title_fullStr Protein denaturation in pilot scale spray dryer
title_full_unstemmed Protein denaturation in pilot scale spray dryer
title_sort protein denaturation in pilot scale spray dryer
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/2696/1/KIOH_SHIN_FANG.PDF
http://umpir.ump.edu.my/id/eprint/2696/
_version_ 1643664683034476544
score 13.211869

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