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Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane

Separation of whey protein in goat milk by cross flow filtration using hollow fibre membrane is a good way for protein purification Cross flow filtration is a good protein purification method because it can operate continuously and does not require frequent maintenance therefore can be applied fo...

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Main Author: Arumugam, Gayathri
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/2694/1/GAYATHRI_ANK_PERMP_ARUMUQAN.PDF
http://umpir.ump.edu.my/id/eprint/2694/
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spelling my.ump.umpir.26942015-03-03T07:57:59Z http://umpir.ump.edu.my/id/eprint/2694/ Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane Arumugam, Gayathri TP Chemical technology Separation of whey protein in goat milk by cross flow filtration using hollow fibre membrane is a good way for protein purification Cross flow filtration is a good protein purification method because it can operate continuously and does not require frequent maintenance therefore can be applied for industrial use . Protein separation by microfiltration requires pretreatment which includes centrifugation and filtration using Whatman filter paper to remove fat layer . Casein precipitation is done by reducing pH of goat milk to 4.6 to avoid fouling la yer on membrane . Microfiltration is rim using whey solution of varying pH and cross flow velocity to stud y concentration of whey protein in permeate during cross flow filtration . Whey protein concentration in sample collected froth permeate is analysed using Reverse Phase Chromatograpy RPC) where peak obtained is compared with standard to determine whey concentration . pH values selected is from range 6 to 8 and cross flow velocity range from 0.01 cm/s to 0.03 cm/s .. Concentration of whey protein B SA and f - lactoglobulin shows highest value compared to other pH values . pH 6.5 was an optimum pH because .wheyprotein gets denatured at higher pH and gets precipitated at lower pH . Concentration of whey protein is highest at cross flow velocity value of 0.03 cm/s . Whe y protein concentration increases steadilyas cross flow velocity increases . Cross flow filtration is an efficient method for protein purification and can be applied in industry. 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/2694/1/GAYATHRI_ANK_PERMP_ARUMUQAN.PDF Arumugam, Gayathri (2010) Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Arumugam, Gayathri
Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane
description Separation of whey protein in goat milk by cross flow filtration using hollow fibre membrane is a good way for protein purification Cross flow filtration is a good protein purification method because it can operate continuously and does not require frequent maintenance therefore can be applied for industrial use . Protein separation by microfiltration requires pretreatment which includes centrifugation and filtration using Whatman filter paper to remove fat layer . Casein precipitation is done by reducing pH of goat milk to 4.6 to avoid fouling la yer on membrane . Microfiltration is rim using whey solution of varying pH and cross flow velocity to stud y concentration of whey protein in permeate during cross flow filtration . Whey protein concentration in sample collected froth permeate is analysed using Reverse Phase Chromatograpy RPC) where peak obtained is compared with standard to determine whey concentration . pH values selected is from range 6 to 8 and cross flow velocity range from 0.01 cm/s to 0.03 cm/s .. Concentration of whey protein B SA and f - lactoglobulin shows highest value compared to other pH values . pH 6.5 was an optimum pH because .wheyprotein gets denatured at higher pH and gets precipitated at lower pH . Concentration of whey protein is highest at cross flow velocity value of 0.03 cm/s . Whe y protein concentration increases steadilyas cross flow velocity increases . Cross flow filtration is an efficient method for protein purification and can be applied in industry.
format Undergraduates Project Papers
author Arumugam, Gayathri
author_facet Arumugam, Gayathri
author_sort Arumugam, Gayathri
title Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane
title_short Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane
title_full Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane
title_fullStr Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane
title_full_unstemmed Separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane
title_sort separation of goat milk to whey protein by cross flow filtration using hollow fibre membrane
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/2694/1/GAYATHRI_ANK_PERMP_ARUMUQAN.PDF
http://umpir.ump.edu.my/id/eprint/2694/
_version_ 1643664682288939008
score 13.211869

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