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Evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using LC-QTOF-MS

Despite great achievements in the oral health of populations globally, oral health problems remain in many communities all over the world, thus our study aimed to evaluate the antimicrobial activity of Monopterus albus skin mucus against selected oral pathogens. Monopterus albus is Asian swamp eel w...

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Main Authors: Hilles, A. R., Mahmood, Syed, Mohd Arifin, Kaderi, Ridzwan, Hashim
Format: Article
Language:English
Published: Faculty of Biotechnology and Food Sciences 2019
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/25734/1/Evaluation%20of%20the%20antimicrobial%20properties%20of%20eel%20skin.pdf
http://umpir.ump.edu.my/id/eprint/25734/
https://www.jmbfs.org/issue/august_septmeber_2019_vol-9_no1/jmbfs_894_hilles/?issue_id=5954&article_id=24
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spelling my.ump.umpir.257342019-11-22T04:04:03Z http://umpir.ump.edu.my/id/eprint/25734/ Evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using LC-QTOF-MS Hilles, A. R. Mahmood, Syed Mohd Arifin, Kaderi Ridzwan, Hashim TP Chemical technology Despite great achievements in the oral health of populations globally, oral health problems remain in many communities all over the world, thus our study aimed to evaluate the antimicrobial activity of Monopterus albus skin mucus against selected oral pathogens. Monopterus albus is Asian swamp eel with elongated body like snakes. Their skin covered with a thick layer of mucus. They are usually found as a sluggish in the stagnant waters as it has a weak swimming behavior. Although eels are capable of quick movements, but they tend to be lethargic and they rely on stealth swimming movements to capture their prey such as shrimp, frogs and other small fishes. With regard to this matter, aqueous and methanol extracts were prepared to test antimicrobial activities against selected oral pathogens; Gram-positive bacteria i.e. Enterococcus faecalis, Streptococcus pyogenes, Streptococcus mutans, Gram-negative bacteria which are Klebsiella pneumoniae, Pseudomona aeruginosa and fungus pathogens i.e. Candida albicans. The antimicrobial activities were determined by inhibition percentage. The results showed a dramatic decrease in the oral pathogens treated with eel skin mucus methanol extract higher than the aqueous extract. Enterococcus faecalis showed the highest activity while Candida albicans showed the lowest activity. After in-vitro evaluation for eel skin mucus activities, identification study using liquid chromatography-quadrupole-time-offlight mass spectrometry (LC-QTOF-MS) was performed to investigate the compound responsible for the anti-oral pathogens activities, the results showed the presence of salvianolic acid G which strongly corelated with the antimicrobial activity against selected oral pathogens. Results were statistically significant with p < 0.001. In conclusion, the present study revealed that eel skin mucus can be considered as promising source for anti-oral pathogens activities. Faculty of Biotechnology and Food Sciences 2019-08 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/25734/1/Evaluation%20of%20the%20antimicrobial%20properties%20of%20eel%20skin.pdf Hilles, A. R. and Mahmood, Syed and Mohd Arifin, Kaderi and Ridzwan, Hashim (2019) Evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using LC-QTOF-MS. Journal of Microbiology Biotechnology and Food Sciences, 9 (1). pp. 140-143. ISSN 1338-5178 https://www.jmbfs.org/issue/august_septmeber_2019_vol-9_no1/jmbfs_894_hilles/?issue_id=5954&article_id=24
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Hilles, A. R.
Mahmood, Syed
Mohd Arifin, Kaderi
Ridzwan, Hashim
Evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using LC-QTOF-MS
description Despite great achievements in the oral health of populations globally, oral health problems remain in many communities all over the world, thus our study aimed to evaluate the antimicrobial activity of Monopterus albus skin mucus against selected oral pathogens. Monopterus albus is Asian swamp eel with elongated body like snakes. Their skin covered with a thick layer of mucus. They are usually found as a sluggish in the stagnant waters as it has a weak swimming behavior. Although eels are capable of quick movements, but they tend to be lethargic and they rely on stealth swimming movements to capture their prey such as shrimp, frogs and other small fishes. With regard to this matter, aqueous and methanol extracts were prepared to test antimicrobial activities against selected oral pathogens; Gram-positive bacteria i.e. Enterococcus faecalis, Streptococcus pyogenes, Streptococcus mutans, Gram-negative bacteria which are Klebsiella pneumoniae, Pseudomona aeruginosa and fungus pathogens i.e. Candida albicans. The antimicrobial activities were determined by inhibition percentage. The results showed a dramatic decrease in the oral pathogens treated with eel skin mucus methanol extract higher than the aqueous extract. Enterococcus faecalis showed the highest activity while Candida albicans showed the lowest activity. After in-vitro evaluation for eel skin mucus activities, identification study using liquid chromatography-quadrupole-time-offlight mass spectrometry (LC-QTOF-MS) was performed to investigate the compound responsible for the anti-oral pathogens activities, the results showed the presence of salvianolic acid G which strongly corelated with the antimicrobial activity against selected oral pathogens. Results were statistically significant with p < 0.001. In conclusion, the present study revealed that eel skin mucus can be considered as promising source for anti-oral pathogens activities.
format Article
author Hilles, A. R.
Mahmood, Syed
Mohd Arifin, Kaderi
Ridzwan, Hashim
author_facet Hilles, A. R.
Mahmood, Syed
Mohd Arifin, Kaderi
Ridzwan, Hashim
author_sort Hilles, A. R.
title Evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using LC-QTOF-MS
title_short Evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using LC-QTOF-MS
title_full Evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using LC-QTOF-MS
title_fullStr Evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using LC-QTOF-MS
title_full_unstemmed Evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using LC-QTOF-MS
title_sort evaluation of the antimicrobial properties of eel skin mucus from monopterus albus against selected oral pathogens and identification of the anti-oral bioactive compounds using lc-qtof-ms
publisher Faculty of Biotechnology and Food Sciences
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/25734/1/Evaluation%20of%20the%20antimicrobial%20properties%20of%20eel%20skin.pdf
http://umpir.ump.edu.my/id/eprint/25734/
https://www.jmbfs.org/issue/august_septmeber_2019_vol-9_no1/jmbfs_894_hilles/?issue_id=5954&article_id=24
_version_ 1651866935489462272
score 13.211869

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